{"id":{"repo_id":"utswmed","oai_identifier":"oai:utswmed-ir.tdl.org:2152.5/6595"},"canonical_url":"https://search.dev.ndltd.org/etd/utswmed/oai:utswmed-ir.tdl.org:2152.5/6595","repository":{"repo_id":"utswmed","name":"University of Texas Southwestern Medical Center","base_url":"https://utswmed-ir.tdl.org/server/oai/request"},"display":{"title":"Illuminating Endocytic Organelles with pH-Resposive [sic] Nanomaterials","abstract":"Pages xvii-xviii are misnumbered as pages xii-xii.","abstract_html":"Pages xvii-xviii are misnumbered as pages xii-xii.","abstract_has_math":false,"creators":["Wang, Chensu"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["DeBerardinis, Ralph J.","White, Michael A.","Gao, Jinming","Danuser, Gaudenz","Yoo, Hyuntae","Zhong, Qing"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2019,"date_issued":"2019-06-03T19:53:35Z","date_published":"2019-06-03T19:53:35Z","updated_at":"2026-07-24T05:52:34Z","subjects":["Endocytosis","Endosomes","Fluorescent Dyes","Lysosomes","Nanoparticles"],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["1103324616"],"render_values":[{"text":"1103324616","href":null,"code":true}]}]},"links":{"outbound_url":"https://hdl.handle.net/2152.5/6595","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["DeBerardinis, Ralph J.","White, Michael A.","Gao, Jinming","Danuser, Gaudenz","Yoo, Hyuntae","Zhong, Qing"]},{"key":"dc:creator","label":"Author","values":["Wang, Chensu"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2019-06-03T19:53:35Z","2017-05","2017-02-20","May 2017"]},{"key":"dc:type","label":"Dc Type","values":["Thesis","text"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Endocytosis","Endosomes","Fluorescent Dyes","Lysosomes","Nanoparticles"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2152.5/6595","1103324616"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Pages xvii-xviii are misnumbered as pages xii-xii.","Endosomes, lysosomes and related catabolic organelles are a dynamic continuum of vacuolar structures that impact a number of key cell physiological processes that include protein/lipid metabolism, nutrient sensing and cell survival. To support quantitative investigation of these processes in living cells, we have developed a library of ultra-pH sensitive (UPS) fluorescent nanoparticles with chemical properties that allow fine-scale, multiplexed, spatial-temporal perturbation and quantification of catabolic organelle maturation at single organelle resolution. Deployment in cells enabled quantification of the proton accumulation rate in endosomes; illumination of previously unrecognized regulatory mechanisms coupling pH transitions to endosomal coat protein exchange; discovery of distinct pH thresholds required for mTORC1 activation by free amino acids versus proteins; broad-scale characterization of the consequence of endosomal pH transitions on cellular metabolomic profiles; and functionalization of a context-specific metabolic vulnerability in lung cancer cells. These biological applications benchmarked the robustness and adaptability of this nanotechnology-enabled 'detect and perturb' strategy. As a translational application, we leveraged the technology in high-throughput screening assays that successfully identified chemical agents in the promotion of autophagolysosomal activity through TFEB activation. Formulation of these compounds in liver-tropic biodegradable, biocompatible nanoparticles conferred hepatoprotection against diet-induced steatosis in murine models and prolonged survival in Caenorhabditis elegans. These results highlight the therapeutic potential of small-molecule TFEB activators to ameliorate metabolic syndrome and extend lifespan."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Illuminating Endocytic Organelles with pH-Resposive [sic] Nanomaterials","Illuminating Endocytic Organelles with pH-Responsive Nanomaterials"]}]}],"canonical_facts":{"dc:contributor":["DeBerardinis, Ralph J.","White, Michael A.","Gao, Jinming","Danuser, Gaudenz","Yoo, Hyuntae","Zhong, Qing"],"dc:creator":["Wang, Chensu"],"dc:date":["2019-06-03T19:53:35Z","2017-05","2017-02-20","May 2017"],"dc:description":["Pages xvii-xviii are misnumbered as pages xii-xii.","Endosomes, lysosomes and related catabolic organelles are a dynamic continuum of vacuolar structures that impact a number of key cell physiological processes that include protein/lipid metabolism, nutrient sensing and cell survival. To support quantitative investigation of these processes in living cells, we have developed a library of ultra-pH sensitive (UPS) fluorescent nanoparticles with chemical properties that allow fine-scale, multiplexed, spatial-temporal perturbation and quantification of catabolic organelle maturation at single organelle resolution. Deployment in cells enabled quantification of the proton accumulation rate in endosomes; illumination of previously unrecognized regulatory mechanisms coupling pH transitions to endosomal coat protein exchange; discovery of distinct pH thresholds required for mTORC1 activation by free amino acids versus proteins; broad-scale characterization of the consequence of endosomal pH transitions on cellular metabolomic profiles; and functionalization of a context-specific metabolic vulnerability in lung cancer cells. These biological applications benchmarked the robustness and adaptability of this nanotechnology-enabled 'detect and perturb' strategy. As a translational application, we leveraged the technology in high-throughput screening assays that successfully identified chemical agents in the promotion of autophagolysosomal activity through TFEB activation. Formulation of these compounds in liver-tropic biodegradable, biocompatible nanoparticles conferred hepatoprotection against diet-induced steatosis in murine models and prolonged survival in Caenorhabditis elegans. These results highlight the therapeutic potential of small-molecule TFEB activators to ameliorate metabolic syndrome and extend lifespan."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2152.5/6595","1103324616"],"dc:language":["en"],"dc:subject":["Endocytosis","Endosomes","Fluorescent Dyes","Lysosomes","Nanoparticles"],"dc:title":["Illuminating Endocytic Organelles with pH-Resposive [sic] Nanomaterials","Illuminating Endocytic Organelles with pH-Responsive Nanomaterials"],"dc:type":["Thesis","text"]},"updated_at":"2026-07-24T05:52:34Z"}