{"id":{"repo_id":"utswmed","oai_identifier":"oai:utswmed-ir.tdl.org:2152.5/4108"},"canonical_url":"https://search.dev.ndltd.org/etd/utswmed/oai:utswmed-ir.tdl.org:2152.5/4108","repository":{"repo_id":"utswmed","name":"University of Texas Southwestern Medical Center","base_url":"https://utswmed-ir.tdl.org/server/oai/request"},"display":{"title":"The Role of the Human Nuclear Poly(A) Binding Protein in RNA Decay","abstract":"Control of nuclear RNA stability is an important determinant of gene expression, but the factors involved in nuclear RNA decay remain largely unknown in higher eukaryotes. Here, I describe our work showing that polyadenosine (poly(A)) tails can stimulate transcript decay in the nucleus of human cells, a function mediated by the ubiquitous nuclear poly(A) binding protein PABPN1. We show that PABPN1 is required for the degradation of a viral nuclear noncoding RNA as well as an inefficiently exported human mRNA. Importantly, the targeting of RNAs to this decay pathway requires the PABPN1 and poly(A) polymerase (PAP)-dependent extension of the poly(A) tail. Nuclear transcripts with longer poly(A) tails are then selectively degraded by components of the nuclear exosome. I also describe our work showing that PABPN1 and PAP are required for the degradation of a variety of nuclear-retained long noncoding RNAs. Taken together, this work uncovers an important pathway in the turnover of RNAs in the nucleus.","abstract_html":"Control of nuclear RNA stability is an important determinant of gene expression, but the factors involved in nuclear RNA decay remain largely unknown in higher eukaryotes. Here, I describe our work showing that polyadenosine (poly(A)) tails can stimulate transcript decay in the nucleus of human cells, a function mediated by the ubiquitous nuclear poly(A) binding protein PABPN1. We show that PABPN1 is required for the degradation of a viral nuclear noncoding RNA as well as an inefficiently exported human mRNA. Importantly, the targeting of RNAs to this decay pathway requires the PABPN1 and poly(A) polymerase (PAP)-dependent extension of the poly(A) tail. Nuclear transcripts with longer poly(A) tails are then selectively degraded by components of the nuclear exosome. I also describe our work showing that PABPN1 and PAP are required for the degradation of a variety of nuclear-retained long noncoding RNAs. Taken together, this work uncovers an important pathway in the turnover of RNAs in the nucleus.","abstract_has_math":false,"creators":["Bresson, Stefan"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Green, Carla B.","Buszczak, Michael","Liu, Qinghua","Conrad, Nicholas"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2017,"date_issued":"2017-06-02T15:24:57Z","date_published":"2017-06-02T15:24:57Z","updated_at":"2026-07-24T05:52:24Z","subjects":["Cell Nucleus","Poly A","Poly(A)-Binding Protein I","RNA Stability"],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["988778236"],"render_values":[{"text":"988778236","href":null,"code":true}]}]},"links":{"outbound_url":"https://hdl.handle.net/2152.5/4108","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Green, Carla B.","Buszczak, Michael","Liu, Qinghua","Conrad, Nicholas"]},{"key":"dc:creator","label":"Author","values":["Bresson, Stefan"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2017-06-02T15:24:57Z","2015-05","2015-01-26","May 2015","2017-06-02T15:13:08Z"]},{"key":"dc:type","label":"Dc Type","values":["Thesis","text"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Cell Nucleus","Poly A","Poly(A)-Binding Protein I","RNA Stability"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2152.5/4108","988778236"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Control of nuclear RNA stability is an important determinant of gene expression, but the factors involved in nuclear RNA decay remain largely unknown in higher eukaryotes. Here, I describe our work showing that polyadenosine (poly(A)) tails can stimulate transcript decay in the nucleus of human cells, a function mediated by the ubiquitous nuclear poly(A) binding protein PABPN1. We show that PABPN1 is required for the degradation of a viral nuclear noncoding RNA as well as an inefficiently exported human mRNA. Importantly, the targeting of RNAs to this decay pathway requires the PABPN1 and poly(A) polymerase (PAP)-dependent extension of the poly(A) tail. Nuclear transcripts with longer poly(A) tails are then selectively degraded by components of the nuclear exosome. I also describe our work showing that PABPN1 and PAP are required for the degradation of a variety of nuclear-retained long noncoding RNAs. Taken together, this work uncovers an important pathway in the turnover of RNAs in the nucleus."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["The Role of the Human Nuclear Poly(A) Binding Protein in RNA Decay"]}]}],"canonical_facts":{"dc:contributor":["Green, Carla B.","Buszczak, Michael","Liu, Qinghua","Conrad, Nicholas"],"dc:creator":["Bresson, Stefan"],"dc:date":["2017-06-02T15:24:57Z","2015-05","2015-01-26","May 2015","2017-06-02T15:13:08Z"],"dc:description":["Control of nuclear RNA stability is an important determinant of gene expression, but the factors involved in nuclear RNA decay remain largely unknown in higher eukaryotes. Here, I describe our work showing that polyadenosine (poly(A)) tails can stimulate transcript decay in the nucleus of human cells, a function mediated by the ubiquitous nuclear poly(A) binding protein PABPN1. We show that PABPN1 is required for the degradation of a viral nuclear noncoding RNA as well as an inefficiently exported human mRNA. Importantly, the targeting of RNAs to this decay pathway requires the PABPN1 and poly(A) polymerase (PAP)-dependent extension of the poly(A) tail. Nuclear transcripts with longer poly(A) tails are then selectively degraded by components of the nuclear exosome. I also describe our work showing that PABPN1 and PAP are required for the degradation of a variety of nuclear-retained long noncoding RNAs. Taken together, this work uncovers an important pathway in the turnover of RNAs in the nucleus."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2152.5/4108","988778236"],"dc:language":["en"],"dc:subject":["Cell Nucleus","Poly A","Poly(A)-Binding Protein I","RNA Stability"],"dc:title":["The Role of the Human Nuclear Poly(A) Binding Protein in RNA Decay"],"dc:type":["Thesis","text"]},"updated_at":"2026-07-24T05:52:24Z"}