{"id":{"repo_id":"utswmed","oai_identifier":"oai:utswmed-ir.tdl.org:2152.5/356"},"canonical_url":"https://search.dev.ndltd.org/etd/utswmed/oai:utswmed-ir.tdl.org:2152.5/356","repository":{"repo_id":"utswmed","name":"University of Texas Southwestern Medical Center","base_url":"https://utswmed-ir.tdl.org/server/oai/request"},"display":{"title":"Dynamic Scaffolding in a G-Protein Signaling Cascade","abstract":"The file named "mishraprashant.pdf" is the primary dissertation file. Supplemental movie files ("Mishra_Movie_S1.mpg", "Mishra_Movie_S2.mpg") are also available.","abstract_html":"The file named "mishraprashant.pdf" is the primary dissertation file. Supplemental movie files ("Mishra_Movie_S1.mpg", "Mishra_Movie_S2.mpg") are also available.","abstract_has_math":false,"creators":["Mishra, Prashant"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Ranganathan, Rama"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2010,"date_issued":"2010-07-12T17:44:03Z","date_published":"2010-07-12T17:44:03Z","updated_at":"2026-07-24T05:52:29Z","subjects":["PDZ Domains","GTP-Binding Proteins","Drosophila Proteins"],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["754633796"],"render_values":[{"text":"754633796","href":null,"code":true}]}]},"links":{"outbound_url":"https://hdl.handle.net/2152.5/356","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Ranganathan, Rama"]},{"key":"dc:creator","label":"Author","values":["Mishra, Prashant"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2010-07-12T17:44:03Z","2009-06-18"]},{"key":"dc:type","label":"Dc Type","values":["Thesis","Text","dissertation"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["PDZ Domains","GTP-Binding Proteins","Drosophila Proteins"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2152.5/356","754633796"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The file named "mishraprashant.pdf" is the primary dissertation file. Supplemental movie files ("Mishra_Movie_S1.mpg", "Mishra_Movie_S2.mpg") are also available.","The InaD scaffold organizes a multi-protein complex that is essential for proper visual signaling in Drosophila photoreceptor cells. Here we show that one of the InaD PDZ domains (PDZ5) exists in a redox-dependent equilibrium between two conformations- a reduced form which is similar to the structure of other PDZ domains, and an oxidized form in which the ligand-binding site is distorted through formation of a strong intramolecular disulfide bond. We demonstrate transient light-dependent formation of this disulfide bond in vivo, and find that transgenic flies expressing a mutant InaD in which PDZ5 is locked in the reduced state display severe defects in termination of visual responses and visually-mediated reflex behavior. These studies demonstrate a novel conformational switch mechanism for PDZ domain function and suggest that InaD behaves more like a dynamic machine rather than a passive scaffold, regulating signal transduction at the millisecond timescale through cycles of conformational change."]},{"key":"dc:format","label":"Dc Format","values":["Electronic","application/pdf","born digital","video/mpeg"]},{"key":"dc:title","label":"Title","values":["Dynamic Scaffolding in a G-Protein Signaling Cascade"]}]}],"canonical_facts":{"dc:contributor":["Ranganathan, Rama"],"dc:creator":["Mishra, Prashant"],"dc:date":["2010-07-12T17:44:03Z","2009-06-18"],"dc:description":["The file named "mishraprashant.pdf" is the primary dissertation file. Supplemental movie files ("Mishra_Movie_S1.mpg", "Mishra_Movie_S2.mpg") are also available.","The InaD scaffold organizes a multi-protein complex that is essential for proper visual signaling in Drosophila photoreceptor cells. Here we show that one of the InaD PDZ domains (PDZ5) exists in a redox-dependent equilibrium between two conformations- a reduced form which is similar to the structure of other PDZ domains, and an oxidized form in which the ligand-binding site is distorted through formation of a strong intramolecular disulfide bond. We demonstrate transient light-dependent formation of this disulfide bond in vivo, and find that transgenic flies expressing a mutant InaD in which PDZ5 is locked in the reduced state display severe defects in termination of visual responses and visually-mediated reflex behavior. These studies demonstrate a novel conformational switch mechanism for PDZ domain function and suggest that InaD behaves more like a dynamic machine rather than a passive scaffold, regulating signal transduction at the millisecond timescale through cycles of conformational change."],"dc:format":["Electronic","application/pdf","born digital","video/mpeg"],"dc:identifier":["https://hdl.handle.net/2152.5/356","754633796"],"dc:language":["en"],"dc:subject":["PDZ Domains","GTP-Binding Proteins","Drosophila Proteins"],"dc:title":["Dynamic Scaffolding in a G-Protein Signaling Cascade"],"dc:type":["Thesis","Text","dissertation"]},"updated_at":"2026-07-24T05:52:29Z"}