{"id":{"repo_id":"utswmed","oai_identifier":"oai:utswmed-ir.tdl.org:2152.5/10828"},"canonical_url":"https://search.dev.ndltd.org/etd/utswmed/oai:utswmed-ir.tdl.org:2152.5/10828","repository":{"repo_id":"utswmed","name":"University of Texas Southwestern Medical Center","base_url":"https://utswmed-ir.tdl.org/server/oai/request"},"display":{"title":"The Role of Stac3 in Neuromuscular Synapse Elimination and Maturation","abstract":"The general metadata -- e.g., title, author, abstract, subject headings, etc. -- is publicly available, but access to the submitted files is restricted to UT Southwestern campus access and/or authorized UT Southwestern users.","abstract_html":"The general metadata -- e.g., title, author, abstract, subject headings, etc. -- is publicly available, but access to the submitted files is restricted to UT Southwestern campus access and/or authorized UT Southwestern users.","abstract_has_math":false,"creators":["Cajigas Hernandez, Ambar"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Johnson, Jane E.","Lin, Weichun","Xu, Wei","Huber, Kimberly M."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2026,"date_issued":"2026-06-15T19:45:50Z","date_published":"2026-06-15T19:45:50Z","updated_at":"2026-07-24T05:52:22Z","subjects":["Muscle Fibers, Skeletal","Muscle Proteins","Neuromuscular Junction","Synapses","Motor Neurons"],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["1596185258"],"render_values":[{"text":"1596185258","href":null,"code":true}]}]},"links":{"outbound_url":"https://hdl.handle.net/2152.5/10828","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Johnson, Jane E.","Lin, Weichun","Xu, Wei","Huber, Kimberly M."]},{"key":"dc:creator","label":"Author","values":["Cajigas Hernandez, Ambar"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2026-06-15T19:45:50Z","2024-05","May 2024"]},{"key":"dc:type","label":"Dc Type","values":["Thesis","text"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Muscle Fibers, Skeletal","Muscle Proteins","Neuromuscular Junction","Synapses","Motor Neurons"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2152.5/10828","1596185258"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The general metadata -- e.g., title, author, abstract, subject headings, etc. -- is publicly available, but access to the submitted files is restricted to UT Southwestern campus access and/or authorized UT Southwestern users.","The goal of this dissertation is to study the cellular and molecular mechanisms during postnatal development and maturation at the neuromuscular junction (NMJ) by focusing on the role of post-synaptic cells in the NMJ. During NMJ development, multiple motor axons initially converge onto a single end-plate, but later, all except one withdraw, resulting in one axon per end-plate. This process, called synapse elimination, occurs during the first 3 postnatal weeks in mice, with nearly all NMJs becoming mono-innervated by P21. However, the underlying molecular mechanism remains poorly understood. This study investigates the role of STAC3, a skeletal muscle-specific protein, in regulating synapse elimination. Using AAV9-EGFP-Cre, the Stac3 gene was targeted in LAL muscles of Stac3flox/flox mice, leading to STAC3 protein ablation. At P14 and P21, the percentage of poly-innervated NMJs was significantly increased compared to controls, suggesting that STAC3 plays a role for the proper timing of synapse elimination at the NMJ in mice. Additionally, STAC3-deficient muscle fibers were smaller, and a significant number of them contain centrally localized nuclei. These studies lead to a better understanding of how postsynaptic muscle activity regulates the development, function, and maintenance of synapses, and therefore provide insights into the development of future therapeutic strategies for disorders such as congenital myasthenic syndromes, amyotrophic lateral sclerosis, and spinal muscular atrophy."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["The Role of Stac3 in Neuromuscular Synapse Elimination and Maturation"]}]}],"canonical_facts":{"dc:contributor":["Johnson, Jane E.","Lin, Weichun","Xu, Wei","Huber, Kimberly M."],"dc:creator":["Cajigas Hernandez, Ambar"],"dc:date":["2026-06-15T19:45:50Z","2024-05","May 2024"],"dc:description":["The general metadata -- e.g., title, author, abstract, subject headings, etc. -- is publicly available, but access to the submitted files is restricted to UT Southwestern campus access and/or authorized UT Southwestern users.","The goal of this dissertation is to study the cellular and molecular mechanisms during postnatal development and maturation at the neuromuscular junction (NMJ) by focusing on the role of post-synaptic cells in the NMJ. During NMJ development, multiple motor axons initially converge onto a single end-plate, but later, all except one withdraw, resulting in one axon per end-plate. This process, called synapse elimination, occurs during the first 3 postnatal weeks in mice, with nearly all NMJs becoming mono-innervated by P21. However, the underlying molecular mechanism remains poorly understood. This study investigates the role of STAC3, a skeletal muscle-specific protein, in regulating synapse elimination. Using AAV9-EGFP-Cre, the Stac3 gene was targeted in LAL muscles of Stac3flox/flox mice, leading to STAC3 protein ablation. At P14 and P21, the percentage of poly-innervated NMJs was significantly increased compared to controls, suggesting that STAC3 plays a role for the proper timing of synapse elimination at the NMJ in mice. Additionally, STAC3-deficient muscle fibers were smaller, and a significant number of them contain centrally localized nuclei. These studies lead to a better understanding of how postsynaptic muscle activity regulates the development, function, and maintenance of synapses, and therefore provide insights into the development of future therapeutic strategies for disorders such as congenital myasthenic syndromes, amyotrophic lateral sclerosis, and spinal muscular atrophy."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2152.5/10828","1596185258"],"dc:language":["en"],"dc:subject":["Muscle Fibers, Skeletal","Muscle Proteins","Neuromuscular Junction","Synapses","Motor Neurons"],"dc:title":["The Role of Stac3 in Neuromuscular Synapse Elimination and Maturation"],"dc:type":["Thesis","text"]},"updated_at":"2026-07-24T05:52:22Z"}