{"id":{"repo_id":"utswmed","oai_identifier":"oai:utswmed-ir.tdl.org:2152.5/10808"},"canonical_url":"https://search.dev.ndltd.org/etd/utswmed/oai:utswmed-ir.tdl.org:2152.5/10808","repository":{"repo_id":"utswmed","name":"University of Texas Southwestern Medical Center","base_url":"https://utswmed-ir.tdl.org/server/oai/request"},"display":{"title":"Mechanism and Functions of STING-Induced Noncanonical Autophagy","abstract":"The cGAS-STING pathway is a vital component of innate immunity. In addition to its well-established role in inducing proinflammatory cytokines, activation of the pathway also induces noncanonical autophagy. Despite an increasing body of research on STING-induced noncanonical autophagy, our understanding of its mechanisms, and particularly its functions, remains limited. In this study, we demonstrated that STING-induced noncanonical autophagy requires the interaction between V-ATPase and the ATG16L1-WD40 domain on Rab7-coated endolysosomal compartments. Additionally, we unveiled that STING-induced noncanonical autophagy leads to the activation of MiT/TFE transcription factors (TFEB, TFE3, and MITF), promoting endolysosomal biogenesis. Furthermore, we showed that STING-induced noncanonical autophagy induces ALIX-meditated ESCRT machinery recruitment to mitigate endolysosomal perturbation. Our study unraveled the multifaceted functions of STING-induced noncanonical autophagy in regulating endolysosomal homeostasis.","abstract_html":"The cGAS-STING pathway is a vital component of innate immunity. In addition to its well-established role in inducing proinflammatory cytokines, activation of the pathway also induces noncanonical autophagy. Despite an increasing body of research on STING-induced noncanonical autophagy, our understanding of its mechanisms, and particularly its functions, remains limited. In this study, we demonstrated that STING-induced noncanonical autophagy requires the interaction between V-ATPase and the ATG16L1-WD40 domain on Rab7-coated endolysosomal compartments. Additionally, we unveiled that STING-induced noncanonical autophagy leads to the activation of MiT/TFE transcription factors (TFEB, TFE3, and MITF), promoting endolysosomal biogenesis. Furthermore, we showed that STING-induced noncanonical autophagy induces ALIX-meditated ESCRT machinery recruitment to mitigate endolysosomal perturbation. Our study unraveled the multifaceted functions of STING-induced noncanonical autophagy in regulating endolysosomal homeostasis.","abstract_has_math":false,"creators":["Huang, Tuozhi"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Liu, Yi","Chen, Zhijian J.","Beutler, Bruce","Cobb, Melanie H."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2026,"date_issued":"2026-06-15T19:42:39Z","date_published":"2026-06-15T19:42:39Z","updated_at":"2026-07-24T05:52:36Z","subjects":["Autophagy","Endosomes","Homeostasis","Lysosomes","Membrane Proteins"],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["1596185270"],"render_values":[{"text":"1596185270","href":null,"code":true}]}]},"links":{"outbound_url":"https://hdl.handle.net/2152.5/10808","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Liu, Yi","Chen, Zhijian J.","Beutler, Bruce","Cobb, Melanie H."]},{"key":"dc:creator","label":"Author","values":["Huang, Tuozhi"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2026-06-15T19:42:39Z","2024-05","May 2024","2026-06-15T19:42:40Z"]},{"key":"dc:type","label":"Dc Type","values":["Thesis","text"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Autophagy","Endosomes","Homeostasis","Lysosomes","Membrane Proteins"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2152.5/10808","1596185270"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The cGAS-STING pathway is a vital component of innate immunity. In addition to its well-established role in inducing proinflammatory cytokines, activation of the pathway also induces noncanonical autophagy. Despite an increasing body of research on STING-induced noncanonical autophagy, our understanding of its mechanisms, and particularly its functions, remains limited. In this study, we demonstrated that STING-induced noncanonical autophagy requires the interaction between V-ATPase and the ATG16L1-WD40 domain on Rab7-coated endolysosomal compartments. Additionally, we unveiled that STING-induced noncanonical autophagy leads to the activation of MiT/TFE transcription factors (TFEB, TFE3, and MITF), promoting endolysosomal biogenesis. Furthermore, we showed that STING-induced noncanonical autophagy induces ALIX-meditated ESCRT machinery recruitment to mitigate endolysosomal perturbation. Our study unraveled the multifaceted functions of STING-induced noncanonical autophagy in regulating endolysosomal homeostasis."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Mechanism and Functions of STING-Induced Noncanonical Autophagy"]}]}],"canonical_facts":{"dc:contributor":["Liu, Yi","Chen, Zhijian J.","Beutler, Bruce","Cobb, Melanie H."],"dc:creator":["Huang, Tuozhi"],"dc:date":["2026-06-15T19:42:39Z","2024-05","May 2024","2026-06-15T19:42:40Z"],"dc:description":["The cGAS-STING pathway is a vital component of innate immunity. In addition to its well-established role in inducing proinflammatory cytokines, activation of the pathway also induces noncanonical autophagy. Despite an increasing body of research on STING-induced noncanonical autophagy, our understanding of its mechanisms, and particularly its functions, remains limited. In this study, we demonstrated that STING-induced noncanonical autophagy requires the interaction between V-ATPase and the ATG16L1-WD40 domain on Rab7-coated endolysosomal compartments. Additionally, we unveiled that STING-induced noncanonical autophagy leads to the activation of MiT/TFE transcription factors (TFEB, TFE3, and MITF), promoting endolysosomal biogenesis. Furthermore, we showed that STING-induced noncanonical autophagy induces ALIX-meditated ESCRT machinery recruitment to mitigate endolysosomal perturbation. Our study unraveled the multifaceted functions of STING-induced noncanonical autophagy in regulating endolysosomal homeostasis."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2152.5/10808","1596185270"],"dc:language":["en"],"dc:subject":["Autophagy","Endosomes","Homeostasis","Lysosomes","Membrane Proteins"],"dc:title":["Mechanism and Functions of STING-Induced Noncanonical Autophagy"],"dc:type":["Thesis","text"]},"updated_at":"2026-07-24T05:52:36Z"}