{"id":{"repo_id":"utswmed","oai_identifier":"oai:utswmed-ir.tdl.org:2152.5/10457"},"canonical_url":"https://search.dev.ndltd.org/etd/utswmed/oai:utswmed-ir.tdl.org:2152.5/10457","repository":{"repo_id":"utswmed","name":"University of Texas Southwestern Medical Center","base_url":"https://utswmed-ir.tdl.org/server/oai/request"},"display":{"title":"Investigating the Function of an Actin Nucleation-Promoting Factor N-WASP in Mammalian Myoblast Fusion","abstract":"Pages 61-89 are misnumbered as pages 75-103, and pages 90-100 are misnumbered as pages 102-112.","abstract_html":"Pages 61-89 are misnumbered as pages 75-103, and pages 90-100 are misnumbered as pages 102-112.","abstract_has_math":false,"creators":["Walji, Tezin A."],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Zhu, Hao","Chen, Elizabeth","Munshi, Nikhil","Cleaver, Ondine"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2025,"date_issued":"2025-01-02T21:58:20Z","date_published":"2025-01-02T21:58:20Z","updated_at":"2026-07-24T05:52:13Z","subjects":["Cell Differentiation","Gene Expression Regulation, Developmental","Muscle, Skeletal","Muscles","Myoblasts","Wiskott-Aldrich Syndrome Protein, Neuronal","Actins"],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["1482732264"],"render_values":[{"text":"1482732264","href":null,"code":true}]}]},"links":{"outbound_url":"https://hdl.handle.net/2152.5/10457","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Zhu, Hao","Chen, Elizabeth","Munshi, Nikhil","Cleaver, Ondine"]},{"key":"dc:creator","label":"Author","values":["Walji, Tezin A."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2025-01-02T21:58:20Z","2022-12","December 2022"]},{"key":"dc:type","label":"Dc Type","values":["Thesis","text"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Cell Differentiation","Gene Expression Regulation, Developmental","Muscle, Skeletal","Muscles","Myoblasts","Wiskott-Aldrich Syndrome Protein, Neuronal","Actins"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2152.5/10457","1482732264"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Pages 61-89 are misnumbered as pages 75-103, and pages 90-100 are misnumbered as pages 102-112.","Skeletal muscle makes up ~ 40% of the total body weight. For mature, functioning muscle to develop from undifferentiated cells, several processes must be coordinated. During myogenesis, muscle progenitor cells activate a muscle-specific genetic program that restricts their cell fate to the myogenic lineage, exiting the cell cycle and differentiating into myocytes. Fusion between myocytes leads to the generation of multinucleated syncytial cells called myotubes which grow and form mature myofibers. Cell-cell fusion is the underlying process for skeletal muscle development and regeneration. While Drosophila has been used extensively to study myoblast fusion, little is known about the site of fusion in mammalian myoblast fusion. Through my work, I show that invasive finger-like protrusions are present during fusion in vitro and in vivo during development. I investigate the role of nucleation promoting factor, N-WASP, in C2C12 cells and in mouse models. N-WASP is present at the site of fusion prior to a fusion event and is involved in protrusion formation. Lastly, in vivo mouse models result in a mild muscle phenotype."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Investigating the Function of an Actin Nucleation-Promoting Factor N-WASP in Mammalian Myoblast Fusion"]}]}],"canonical_facts":{"dc:contributor":["Zhu, Hao","Chen, Elizabeth","Munshi, Nikhil","Cleaver, Ondine"],"dc:creator":["Walji, Tezin A."],"dc:date":["2025-01-02T21:58:20Z","2022-12","December 2022"],"dc:description":["Pages 61-89 are misnumbered as pages 75-103, and pages 90-100 are misnumbered as pages 102-112.","Skeletal muscle makes up ~ 40% of the total body weight. For mature, functioning muscle to develop from undifferentiated cells, several processes must be coordinated. During myogenesis, muscle progenitor cells activate a muscle-specific genetic program that restricts their cell fate to the myogenic lineage, exiting the cell cycle and differentiating into myocytes. Fusion between myocytes leads to the generation of multinucleated syncytial cells called myotubes which grow and form mature myofibers. Cell-cell fusion is the underlying process for skeletal muscle development and regeneration. While Drosophila has been used extensively to study myoblast fusion, little is known about the site of fusion in mammalian myoblast fusion. Through my work, I show that invasive finger-like protrusions are present during fusion in vitro and in vivo during development. I investigate the role of nucleation promoting factor, N-WASP, in C2C12 cells and in mouse models. N-WASP is present at the site of fusion prior to a fusion event and is involved in protrusion formation. Lastly, in vivo mouse models result in a mild muscle phenotype."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2152.5/10457","1482732264"],"dc:language":["en"],"dc:subject":["Cell Differentiation","Gene Expression Regulation, Developmental","Muscle, Skeletal","Muscles","Myoblasts","Wiskott-Aldrich Syndrome Protein, Neuronal","Actins"],"dc:title":["Investigating the Function of an Actin Nucleation-Promoting Factor N-WASP in Mammalian Myoblast Fusion"],"dc:type":["Thesis","text"]},"updated_at":"2026-07-24T05:52:13Z"}