{"id":{"repo_id":"utswmed","oai_identifier":"oai:utswmed-ir.tdl.org:2152.5/10438"},"canonical_url":"https://search.dev.ndltd.org/etd/utswmed/oai:utswmed-ir.tdl.org:2152.5/10438","repository":{"repo_id":"utswmed","name":"University of Texas Southwestern Medical Center","base_url":"https://utswmed-ir.tdl.org/server/oai/request"},"display":{"title":"Mycobacterium tuberculosis Lipids Activate Neurons and Trigger Cough","abstract":"Pulmonary tuberculosis, a disease caused by Mycobacterium tuberculosis (Mtb), manifests with a persistent cough as both a primary symptom and a transmission mechanism. While nociceptive neurons innervating the lungs trigger the cough reflex and some bacteria produce neuron-targeting molecules, how pulmonary Mtb infection causes cough remains undefined, and whether Mtb produces a neuron-activating molecule that induces cough is unknown. Here, I identified the Mtb glycolipid sulfolipid-1 (SL-1) as a nociceptive-activating and cough-inducing molecule. I also demonstrated that phenolic glycolipid (PGL), which is uniquely produced by the hypervirulent Mtb HN878, activates nociceptive neurons. Finally, I determined that a GPCR mediates SL-1 activity, independent of TRPV1. This work demonstrates a novel molecular mechanism for cough induction by a virulent human pathogen via its production of complex lipids to induce a cough response.","abstract_html":"Pulmonary tuberculosis, a disease caused by Mycobacterium tuberculosis (Mtb), manifests with a persistent cough as both a primary symptom and a transmission mechanism. While nociceptive neurons innervating the lungs trigger the cough reflex and some bacteria produce neuron-targeting molecules, how pulmonary Mtb infection causes cough remains undefined, and whether Mtb produces a neuron-activating molecule that induces cough is unknown. Here, I identified the Mtb glycolipid sulfolipid-1 (SL-1) as a nociceptive-activating and cough-inducing molecule. I also demonstrated that phenolic glycolipid (PGL), which is uniquely produced by the hypervirulent Mtb HN878, activates nociceptive neurons. Finally, I determined that a GPCR mediates SL-1 activity, independent of TRPV1. This work demonstrates a novel molecular mechanism for cough induction by a virulent human pathogen via its production of complex lipids to induce a cough response.","abstract_has_math":false,"creators":["Ruhl, Cody Ryan"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Kohler, Jennifer J.","Lai, Helen","Tu, Benjamin","Shiloh, Michael"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2025,"date_issued":"2025-01-02T21:57:03Z","date_published":"2025-01-02T21:57:03Z","updated_at":"2026-07-24T05:52:31Z","subjects":["Cough","Glycolipids","Nociceptors","Virulence Factors"],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["1482732312"],"render_values":[{"text":"1482732312","href":null,"code":true}]}]},"links":{"outbound_url":"https://hdl.handle.net/2152.5/10438","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Kohler, Jennifer J.","Lai, Helen","Tu, Benjamin","Shiloh, Michael"]},{"key":"dc:creator","label":"Author","values":["Ruhl, Cody Ryan"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2025-01-02T21:57:03Z","2022-12","December 2022","2025-01-02T21:57:04Z"]},{"key":"dc:type","label":"Dc Type","values":["Thesis","text"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Cough","Glycolipids","Nociceptors","Virulence Factors"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2152.5/10438","1482732312"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Pulmonary tuberculosis, a disease caused by Mycobacterium tuberculosis (Mtb), manifests with a persistent cough as both a primary symptom and a transmission mechanism. While nociceptive neurons innervating the lungs trigger the cough reflex and some bacteria produce neuron-targeting molecules, how pulmonary Mtb infection causes cough remains undefined, and whether Mtb produces a neuron-activating molecule that induces cough is unknown. Here, I identified the Mtb glycolipid sulfolipid-1 (SL-1) as a nociceptive-activating and cough-inducing molecule. I also demonstrated that phenolic glycolipid (PGL), which is uniquely produced by the hypervirulent Mtb HN878, activates nociceptive neurons. Finally, I determined that a GPCR mediates SL-1 activity, independent of TRPV1. This work demonstrates a novel molecular mechanism for cough induction by a virulent human pathogen via its production of complex lipids to induce a cough response."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Mycobacterium tuberculosis Lipids Activate Neurons and Trigger Cough"]}]}],"canonical_facts":{"dc:contributor":["Kohler, Jennifer J.","Lai, Helen","Tu, Benjamin","Shiloh, Michael"],"dc:creator":["Ruhl, Cody Ryan"],"dc:date":["2025-01-02T21:57:03Z","2022-12","December 2022","2025-01-02T21:57:04Z"],"dc:description":["Pulmonary tuberculosis, a disease caused by Mycobacterium tuberculosis (Mtb), manifests with a persistent cough as both a primary symptom and a transmission mechanism. While nociceptive neurons innervating the lungs trigger the cough reflex and some bacteria produce neuron-targeting molecules, how pulmonary Mtb infection causes cough remains undefined, and whether Mtb produces a neuron-activating molecule that induces cough is unknown. Here, I identified the Mtb glycolipid sulfolipid-1 (SL-1) as a nociceptive-activating and cough-inducing molecule. I also demonstrated that phenolic glycolipid (PGL), which is uniquely produced by the hypervirulent Mtb HN878, activates nociceptive neurons. Finally, I determined that a GPCR mediates SL-1 activity, independent of TRPV1. This work demonstrates a novel molecular mechanism for cough induction by a virulent human pathogen via its production of complex lipids to induce a cough response."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2152.5/10438","1482732312"],"dc:language":["en"],"dc:subject":["Cough","Glycolipids","Nociceptors","Virulence Factors"],"dc:title":["Mycobacterium tuberculosis Lipids Activate Neurons and Trigger Cough"],"dc:type":["Thesis","text"]},"updated_at":"2026-07-24T05:52:31Z"}