Dynamic and kinetic investigations of SEM-5/SOS peptide interactions

The dynamics of SEM-5 SH3 domain protein can provide an important insight into how structural dynamics contribute to protein function. This is important because solving the structure of protein alone only provides the detail on the population weight average of native ensembles. NMR (Nuclear Magnetic Resonance) is one of the best techniques that can be used to elucidate the conformational fluctuation of SH3 domain under native condition, The hypothesis of this study is that the protein during ligand-free ¬form is undergoing an equilibrium between binding-competent (BC) and binding-incompetent (BI) states(1). In order to test this hypothesis, we apply a Glycine mutation in a solvent exposed and highly dynamic region within RT loop of SH3 domain protein. Through this mutation, we aim to manipulate the dynamics of the protein but preserve the protein-ligand interface. Several NMR methodologies including order parameter and line-shape analysis are applied to observe the correlation of protein dynamics with its function, taking into consideration several thermodynamic and kinetic aspects of the structural changes involved.\r\n\r\n

Name thesis:degree_name

Master of Science

Level thesis:degree_level

Master

Grantor

The University of Texas Medical Branch

Year dc:date.issued

2009

Author dc:creator

• li chieh Chen

Advisor dc:contributor.advisor

• Jose M. Barral

Committee members dc:contributor.committeemember

• Vincent J. Hilser
• Juniji Iwahara

• SH3 domainprotein
• NMR
• line-shape analysis
• kinetics

Statement dc:rights

• Copyright © is held by the author. Presentation of this material on the TDL web site by The University of Texas Medical Branch at Galveston was made possible under a limited license grant from the author who has retained all copyrights in the works.

Language dc:language.iso

eng

Dc Identifier Other

etd-04012009-154648

OAI identifier oai:identifier

oai:utmb-ir.tdl.org:2152.3/85