{"id":{"repo_id":"utmb","oai_identifier":"oai:utmb-ir.tdl.org:2152.3/850"},"canonical_url":"https://search.dev.ndltd.org/etd/utmb/oai:utmb-ir.tdl.org:2152.3/850","repository":{"repo_id":"utmb","name":"University of Texas Medical Branch","base_url":"https://utmb-ir.tdl.org/server/oai/request"},"display":{"title":"Differences in miR-122 and miR-191 Expression in HBV- versus HCV-associated Hepatocellular Carcinoma","abstract":"Prior studies have suggested that the expression of miR-122 and miR-191 is decreased and increased, respectively, in hepatocellular carcinoma. However, other evidence suggests miR-122 may be preserved in HCV-associated hepatocellular carcinoma (HCC). The goal of this thesis is to assess miR-122 and miR-191 expression levels in HBV- and HCV-associated HCC, the related non-tumor tissue, and normal liver tissue. Relative quantification of miR-122 and miR-191 in 16 HCV-associated HCC, 10 HBV-associated HCC, the respective neighboring non-tumor tissue, and 9 normal liver tissue samples was performed using RT-PCR. This thesis shows that miR-122 expression levels are maintained in HCV-associated HCC and HBV-non-tumor tissues, but down-regulated in HBV-associated HCC and HCV-non-tumor tissues compared to normal liver tissue. miR-191 was found only to be up-regulated in HBV-associated HCC compared to normal liver expression levels. Furthermore, the miR-122 expression level in HCV-non-tumor tissue was found to have a relationship with rs8099917, a SNP known to predict HCV treatment outcome. miR-122 expression levels were decreased in patients with the TG genotype, unfavorable to HCV treatment, compared to those with the TT genotype, favorable to HCV treatment. A negative correlation of interferon-stimulated gene expression and miR-122 expression was found using Spearman correlation tests. The differences in miR-122 and miR-191 expression levels in HBV- and HCV-associated HCC hint that there are virus-specific mechanisms that influence liver carcinogenesis during chronic infection.","abstract_html":"Prior studies have suggested that the expression of miR-122 and miR-191 is decreased and increased, respectively, in hepatocellular carcinoma. However, other evidence suggests miR-122 may be preserved in HCV-associated hepatocellular carcinoma (HCC). The goal of this thesis is to assess miR-122 and miR-191 expression levels in HBV- and HCV-associated HCC, the related non-tumor tissue, and normal liver tissue. Relative quantification of miR-122 and miR-191 in 16 HCV-associated HCC, 10 HBV-associated HCC, the respective neighboring non-tumor tissue, and 9 normal liver tissue samples was performed using RT-PCR. This thesis shows that miR-122 expression levels are maintained in HCV-associated HCC and HBV-non-tumor tissues, but down-regulated in HBV-associated HCC and HCV-non-tumor tissues compared to normal liver tissue. miR-191 was found only to be up-regulated in HBV-associated HCC compared to normal liver expression levels. Furthermore, the miR-122 expression level in HCV-non-tumor tissue was found to have a relationship with rs8099917, a SNP known to predict HCV treatment outcome. miR-122 expression levels were decreased in patients with the TG genotype, unfavorable to HCV treatment, compared to those with the TT genotype, favorable to HCV treatment. A negative correlation of interferon-stimulated gene expression and miR-122 expression was found using Spearman correlation tests. The differences in miR-122 and miR-191 expression levels in HBV- and HCV-associated HCC hint that there are virus-specific mechanisms that influence liver carcinogenesis during chronic infection.","abstract_has_math":false,"creators":["Spaniel, Carolyn"],"institution":"The University of Texas Medical Branch at Galveston","degree_name":"Microbiology and Immunology (Masters)","degree_level":"Masters","degree_discipline":"Virology","degree_department":null,"school":null,"contributors":[],"advisors":["Lemon, Stanley M"],"committee_chairs":[],"committee_members":["Bachenheimer, Steven L","Yi, MinKyung"],"year":null,"date_issued":"","date_published":null,"updated_at":"2026-07-24T05:50:54Z","subjects":["miR-122, miR-191, hepatocellular carcinoma, interferon, liver cancer"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2152.3/850","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Lemon, Stanley M"]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Bachenheimer, Steven L","Yi, MinKyung"]},{"key":"dc:creator","label":"Author","values":["Spaniel, Carolyn"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2016-11-14T15:24:47Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2016-11-14T15:24:47Z"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Virology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Microbiology and Immunology (Masters)"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["The University of Texas Medical Branch at Galveston"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["miR-122, miR-191, hepatocellular carcinoma, interferon, liver cancer"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/2152.3/850"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Prior studies have suggested that the expression of miR-122 and miR-191 is decreased and increased, respectively, in hepatocellular carcinoma. However, other evidence suggests miR-122 may be preserved in HCV-associated hepatocellular carcinoma (HCC). The goal of this thesis is to assess miR-122 and miR-191 expression levels in HBV- and HCV-associated HCC, the related non-tumor tissue, and normal liver tissue. Relative quantification of miR-122 and miR-191 in 16 HCV-associated HCC, 10 HBV-associated HCC, the respective neighboring non-tumor tissue, and 9 normal liver tissue samples was performed using RT-PCR. This thesis shows that miR-122 expression levels are maintained in HCV-associated HCC and HBV-non-tumor tissues, but down-regulated in HBV-associated HCC and HCV-non-tumor tissues compared to normal liver tissue. miR-191 was found only to be up-regulated in HBV-associated HCC compared to normal liver expression levels. Furthermore, the miR-122 expression level in HCV-non-tumor tissue was found to have a relationship with rs8099917, a SNP known to predict HCV treatment outcome. miR-122 expression levels were decreased in patients with the TG genotype, unfavorable to HCV treatment, compared to those with the TT genotype, favorable to HCV treatment. A negative correlation of interferon-stimulated gene expression and miR-122 expression was found using Spearman correlation tests. The differences in miR-122 and miR-191 expression levels in HBV- and HCV-associated HCC hint that there are virus-specific mechanisms that influence liver carcinogenesis during chronic infection."]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Differences in miR-122 and miR-191 Expression in HBV- versus HCV-associated Hepatocellular Carcinoma"]}]}],"canonical_facts":{"dc:contributor.advisor":["Lemon, Stanley M"],"dc:contributor.committeemember":["Bachenheimer, Steven L","Yi, MinKyung"],"dc:creator":["Spaniel, Carolyn"],"dc:date.accessioned":["2016-11-14T15:24:47Z"],"dc:date.available":["2016-11-14T15:24:47Z"],"dc:description.abstract":["Prior studies have suggested that the expression of miR-122 and miR-191 is decreased and increased, respectively, in hepatocellular carcinoma. However, other evidence suggests miR-122 may be preserved in HCV-associated hepatocellular carcinoma (HCC). The goal of this thesis is to assess miR-122 and miR-191 expression levels in HBV- and HCV-associated HCC, the related non-tumor tissue, and normal liver tissue. Relative quantification of miR-122 and miR-191 in 16 HCV-associated HCC, 10 HBV-associated HCC, the respective neighboring non-tumor tissue, and 9 normal liver tissue samples was performed using RT-PCR. This thesis shows that miR-122 expression levels are maintained in HCV-associated HCC and HBV-non-tumor tissues, but down-regulated in HBV-associated HCC and HCV-non-tumor tissues compared to normal liver tissue. miR-191 was found only to be up-regulated in HBV-associated HCC compared to normal liver expression levels. Furthermore, the miR-122 expression level in HCV-non-tumor tissue was found to have a relationship with rs8099917, a SNP known to predict HCV treatment outcome. miR-122 expression levels were decreased in patients with the TG genotype, unfavorable to HCV treatment, compared to those with the TT genotype, favorable to HCV treatment. A negative correlation of interferon-stimulated gene expression and miR-122 expression was found using Spearman correlation tests. The differences in miR-122 and miR-191 expression levels in HBV- and HCV-associated HCC hint that there are virus-specific mechanisms that influence liver carcinogenesis during chronic infection."],"dc:format.mimetype":["application/pdf"],"dc:identifier.uri":["http://hdl.handle.net/2152.3/850"],"dc:subject":["miR-122, miR-191, hepatocellular carcinoma, interferon, liver cancer"],"dc:title":["Differences in miR-122 and miR-191 Expression in HBV- versus HCV-associated Hepatocellular Carcinoma"],"dc:type":["Thesis"],"thesis:degree_discipline":["Virology"],"thesis:degree_level":["Masters"],"thesis:degree_name":["Microbiology and Immunology (Masters)"],"thesis:institution_name":["The University of Texas Medical Branch at Galveston"]},"updated_at":"2026-07-24T05:50:54Z"}