{"id":{"repo_id":"utmb","oai_identifier":"oai:utmb-ir.tdl.org:2152.3/198"},"canonical_url":"https://search.dev.ndltd.org/etd/utmb/oai:utmb-ir.tdl.org:2152.3/198","repository":{"repo_id":"utmb","name":"University of Texas Medical Branch","base_url":"https://utmb-ir.tdl.org/server/oai/request"},"display":{"title":"Use of surface enhanced laser desorption/ionization-time of flight mass spectrometry (SELDI-TOF MS) to study protein expression in a rat model of cocaine withdrawal","abstract":"SELDI-TOF MS is an analytical technology that combines chromatography and mass spectrometry. It is being exploited for the measurement of numerous proteins in serum, less often in organ tissue (rarely in brain). We applied SELDI-TOF MS technology to study protein expression in the brain of rats withdrawn from repeated cocaine exposure. Our goals were to optimize sample preparation and ProteinChip„¥ Array protocols for brain tissue, to verify the reproducibility of SELDI-TOF mass spectra, and to determine whether SELDI-TOF MS detects differentially expressed proteins in cocaine- vs. saline-treated rats. Consequently, we have developed an optimal protocol and generated a reproducible spectra with six dominant peaks in all test samples. We have found two peaks (m/z: 5179, 5030) significantly increased (p <. 05) in cocaine-treated rats. In summary, the application of SELDI-TOF MS to the study of protein expression in a rat model of cocaine withdrawal is feasible and potentially informative.","abstract_html":"SELDI-TOF MS is an analytical technology that combines chromatography and mass spectrometry. It is being exploited for the measurement of numerous proteins in serum, less often in organ tissue (rarely in brain). We applied SELDI-TOF MS technology to study protein expression in the brain of rats withdrawn from repeated cocaine exposure. Our goals were to optimize sample preparation and ProteinChip„¥ Array protocols for brain tissue, to verify the reproducibility of SELDI-TOF mass spectra, and to determine whether SELDI-TOF MS detects differentially expressed proteins in cocaine- vs. saline-treated rats. Consequently, we have developed an optimal protocol and generated a reproducible spectra with six dominant peaks in all test samples. We have found two peaks (m/z: 5179, 5030) significantly increased (p <. 05) in cocaine-treated rats. In summary, the application of SELDI-TOF MS to the study of protein expression in a rat model of cocaine withdrawal is feasible and potentially informative.","abstract_has_math":false,"creators":["Tao Geng"],"institution":"The University of Texas Medical Branch","degree_name":"Master of Science","degree_level":"Master","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Kathryn A. Cunningham"],"committee_chairs":[],"committee_members":["Xiaodong Cheng","Norbert K. Herzog","Mary L. Thomas"],"year":2005,"date_issued":"2005-07-22","date_published":"2005-07-22","updated_at":"2026-07-24T05:50:59Z","subjects":["proteomics brain"],"languages":["eng"],"rights":["Copyright © is held by the author. Presentation of this material on the TDL web site by The University of Texas Medical Branch at Galveston was made possible under a limited license grant from the author who has retained all copyrights in the works."],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-07292005-113525"],"render_values":[{"text":"etd-07292005-113525","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2152.3/198","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Kathryn A. Cunningham"]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Xiaodong Cheng","Norbert K. Herzog","Mary L. Thomas"]},{"key":"dc:creator","label":"Author","values":["Tao Geng"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2011-12-20T16:05:08Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2008-06-17","2011-12-20T16:05:08Z"]},{"key":"dc:date.issued","label":"Date","values":["2005-07-22"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Master"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["The University of Texas Medical Branch"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["proteomics brain"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright © is held by the author. Presentation of this material on the TDL web site by The University of Texas Medical Branch at Galveston was made possible under a limited license grant from the author who has retained all copyrights in the works."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.other","label":"Dc Identifier Other","values":["etd-07292005-113525"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/2152.3/198"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["SELDI-TOF MS is an analytical technology that combines chromatography and mass spectrometry. It is being exploited for the measurement of numerous proteins in serum, less often in organ tissue (rarely in brain). We applied SELDI-TOF MS technology to study protein expression in the brain of rats withdrawn from repeated cocaine exposure. Our goals were to optimize sample preparation and ProteinChip„¥ Array protocols for brain tissue, to verify the reproducibility of SELDI-TOF mass spectra, and to determine whether SELDI-TOF MS detects differentially expressed proteins in cocaine- vs. saline-treated rats. Consequently, we have developed an optimal protocol and generated a reproducible spectra with six dominant peaks in all test samples. We have found two peaks (m/z: 5179, 5030) significantly increased (p <. 05) in cocaine-treated rats. In summary, the application of SELDI-TOF MS to the study of protein expression in a rat model of cocaine withdrawal is feasible and potentially informative."]},{"key":"dc:format.medium","label":"Dc Format Medium","values":["electronic"]},{"key":"dc:title","label":"Title","values":["Use of surface enhanced laser desorption/ionization-time of flight mass spectrometry (SELDI-TOF MS) to study protein expression in a rat model of cocaine withdrawal"]}]}],"canonical_facts":{"dc:contributor.advisor":["Kathryn A. Cunningham"],"dc:contributor.committeemember":["Xiaodong Cheng","Norbert K. Herzog","Mary L. Thomas"],"dc:creator":["Tao Geng"],"dc:date.accessioned":["2011-12-20T16:05:08Z"],"dc:date.available":["2008-06-17","2011-12-20T16:05:08Z"],"dc:date.issued":["2005-07-22"],"dc:description.abstract":["SELDI-TOF MS is an analytical technology that combines chromatography and mass spectrometry. It is being exploited for the measurement of numerous proteins in serum, less often in organ tissue (rarely in brain). We applied SELDI-TOF MS technology to study protein expression in the brain of rats withdrawn from repeated cocaine exposure. Our goals were to optimize sample preparation and ProteinChip„¥ Array protocols for brain tissue, to verify the reproducibility of SELDI-TOF mass spectra, and to determine whether SELDI-TOF MS detects differentially expressed proteins in cocaine- vs. saline-treated rats. Consequently, we have developed an optimal protocol and generated a reproducible spectra with six dominant peaks in all test samples. We have found two peaks (m/z: 5179, 5030) significantly increased (p <. 05) in cocaine-treated rats. In summary, the application of SELDI-TOF MS to the study of protein expression in a rat model of cocaine withdrawal is feasible and potentially informative."],"dc:format.medium":["electronic"],"dc:identifier.other":["etd-07292005-113525"],"dc:identifier.uri":["http://hdl.handle.net/2152.3/198"],"dc:language.iso":["eng"],"dc:rights":["Copyright © is held by the author. Presentation of this material on the TDL web site by The University of Texas Medical Branch at Galveston was made possible under a limited license grant from the author who has retained all copyrights in the works."],"dc:subject":["proteomics brain"],"dc:title":["Use of surface enhanced laser desorption/ionization-time of flight mass spectrometry (SELDI-TOF MS) to study protein expression in a rat model of cocaine withdrawal"],"thesis:degree_level":["Master"],"thesis:degree_name":["Master of Science"],"thesis:institution_name":["The University of Texas Medical Branch"]},"updated_at":"2026-07-24T05:50:59Z"}