{"id":{"repo_id":"utmb","oai_identifier":"oai:utmb-ir.tdl.org:2152.3/12669"},"canonical_url":"https://search.dev.ndltd.org/etd/utmb/oai:utmb-ir.tdl.org:2152.3/12669","repository":{"repo_id":"utmb","name":"University of Texas Medical Branch","base_url":"https://utmb-ir.tdl.org/server/oai/request"},"display":{"title":"Contribution of Secretion Systems to Macrophage Polarization and Cell Death During Burkholderia pseudomallei Infection","abstract":"Burkholderia pseudomallei is a Gram-negative environmental saprophyte and causative agent of melioidosis. As a facultative intracellular pathogen, B. pseudomallei uses secretion systems as primary virulence factors. The type 3 secretion system (T3SS) is used to escape the phagosome while the type 6 secretion system is responsible for cell-to-cell fusion and multinucleated giant cell (MNGC) formation. Both secretion systems are critical for virulence but the impact they have on the host response is uncharacterized. In this dissertation, we demonstrate the effect these secretion systems have on the immune response by evaluating macrophage polarization and cell death. Using two mutant strains, ∆bicA and ∆hcp1, as case studies, we demonstrate that there is a differential macrophage polarization and apoptosis response. Together, they represent host response elements that are manipulated by the bacteria in a secretion system dependent manner to promote replication within the host.","abstract_html":"Burkholderia pseudomallei is a Gram-negative environmental saprophyte and causative agent of melioidosis. As a facultative intracellular pathogen, B. pseudomallei uses secretion systems as primary virulence factors. The type 3 secretion system (T3SS) is used to escape the phagosome while the type 6 secretion system is responsible for cell-to-cell fusion and multinucleated giant cell (MNGC) formation. Both secretion systems are critical for virulence but the impact they have on the host response is uncharacterized. In this dissertation, we demonstrate the effect these secretion systems have on the immune response by evaluating macrophage polarization and cell death. Using two mutant strains, ∆bicA and ∆hcp1, as case studies, we demonstrate that there is a differential macrophage polarization and apoptosis response. Together, they represent host response elements that are manipulated by the bacteria in a secretion system dependent manner to promote replication within the host.","abstract_has_math":false,"creators":["Stockton, Jacob Lee 1996-"],"institution":"The University of Texas Medical Branch at Galveston","degree_name":"Microbiology and Immunology (Doctoral)","degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Torres, Alfredo altorres@utmb.edu"],"committee_chairs":[],"committee_members":[],"year":2024,"date_issued":"2024-05","date_published":"2024-05","updated_at":"2026-07-24T05:51:09Z","subjects":[],"languages":["English"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2152.3/12669","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Torres, Alfredo altorres@utmb.edu"]},{"key":"dc:creator","label":"Author","values":["Stockton, Jacob Lee 1996-"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2025-04-07T14:40:46Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2025-04-07T14:40:46Z"]},{"key":"dc:date.issued","label":"Date","values":["2024-05"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Microbiology and Immunology (Doctoral)"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["The University of Texas Medical Branch at Galveston"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["English"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/2152.3/12669"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Burkholderia pseudomallei is a Gram-negative environmental saprophyte and causative agent of melioidosis. As a facultative intracellular pathogen, B. pseudomallei uses secretion systems as primary virulence factors. The type 3 secretion system (T3SS) is used to escape the phagosome while the type 6 secretion system is responsible for cell-to-cell fusion and multinucleated giant cell (MNGC) formation. Both secretion systems are critical for virulence but the impact they have on the host response is uncharacterized. In this dissertation, we demonstrate the effect these secretion systems have on the immune response by evaluating macrophage polarization and cell death. Using two mutant strains, ∆bicA and ∆hcp1, as case studies, we demonstrate that there is a differential macrophage polarization and apoptosis response. Together, they represent host response elements that are manipulated by the bacteria in a secretion system dependent manner to promote replication within the host."]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Contribution of Secretion Systems to Macrophage Polarization and Cell Death During Burkholderia pseudomallei Infection"]}]}],"canonical_facts":{"dc:contributor.advisor":["Torres, Alfredo altorres@utmb.edu"],"dc:creator":["Stockton, Jacob Lee 1996-"],"dc:date.accessioned":["2025-04-07T14:40:46Z"],"dc:date.available":["2025-04-07T14:40:46Z"],"dc:date.issued":["2024-05"],"dc:description.abstract":["Burkholderia pseudomallei is a Gram-negative environmental saprophyte and causative agent of melioidosis. As a facultative intracellular pathogen, B. pseudomallei uses secretion systems as primary virulence factors. The type 3 secretion system (T3SS) is used to escape the phagosome while the type 6 secretion system is responsible for cell-to-cell fusion and multinucleated giant cell (MNGC) formation. Both secretion systems are critical for virulence but the impact they have on the host response is uncharacterized. In this dissertation, we demonstrate the effect these secretion systems have on the immune response by evaluating macrophage polarization and cell death. Using two mutant strains, ∆bicA and ∆hcp1, as case studies, we demonstrate that there is a differential macrophage polarization and apoptosis response. Together, they represent host response elements that are manipulated by the bacteria in a secretion system dependent manner to promote replication within the host."],"dc:format.mimetype":["application/pdf"],"dc:identifier.uri":["https://hdl.handle.net/2152.3/12669"],"dc:language.iso":["English"],"dc:title":["Contribution of Secretion Systems to Macrophage Polarization and Cell Death During Burkholderia pseudomallei Infection"],"dc:type":["Thesis"],"thesis:degree_name":["Microbiology and Immunology (Doctoral)"],"thesis:institution_name":["The University of Texas Medical Branch at Galveston"]},"updated_at":"2026-07-24T05:51:09Z"}