{"id":{"repo_id":"utmb","oai_identifier":"oai:utmb-ir.tdl.org:2152.3/11363"},"canonical_url":"https://search.dev.ndltd.org/etd/utmb/oai:utmb-ir.tdl.org:2152.3/11363","repository":{"repo_id":"utmb","name":"University of Texas Medical Branch","base_url":"https://utmb-ir.tdl.org/server/oai/request"},"display":{"title":"THE ROLE OF INTEGRATOR SUBUNIT 11 IN PROMOTOR-PROXIMAL ATTENUATION OF PROTEIN CODING GENES","abstract":"The Integrator complex is a 14-subunit protein complex that interacts with RNA polymerase II (RNAPII) in metazoans during transcription of multiple forms of coding and non-coding RNA. Originally described as being responsible for the 3’ cleavage and termination of uridine-rich small nuclear RNA, further roles have been discovered in the cleavage of long non-coding RNA, enhancer RNA, telomerase RNA, and coding messenger RNAs. While many studies have identified a presence at the transcriptional start site of mRNA, Integrator’s function at these mRNA has yet to be identified. In this study, we used multiple biochemical and sequencing techniques to elucidate a role for Integrator at these mRNA start sites. We found that the endonuclease activity of Integrator subunit 11 (IntS11) was responsible for the attenuation of mRNA through promoter-proximal termination of a large set of paused mRNAs. We also identified a novel interaction between IntS11 and CG7044 that could inhibit IntS11’s endonuclease activity.","abstract_html":"The Integrator complex is a 14-subunit protein complex that interacts with RNA polymerase II (RNAPII) in metazoans during transcription of multiple forms of coding and non-coding RNA. Originally described as being responsible for the 3’ cleavage and termination of uridine-rich small nuclear RNA, further roles have been discovered in the cleavage of long non-coding RNA, enhancer RNA, telomerase RNA, and coding messenger RNAs. While many studies have identified a presence at the transcriptional start site of mRNA, Integrator’s function at these mRNA has yet to be identified. In this study, we used multiple biochemical and sequencing techniques to elucidate a role for Integrator at these mRNA start sites. We found that the endonuclease activity of Integrator subunit 11 (IntS11) was responsible for the attenuation of mRNA through promoter-proximal termination of a large set of paused mRNAs. We also identified a novel interaction between IntS11 and CG7044 that could inhibit IntS11’s endonuclease activity.","abstract_has_math":false,"creators":["Elrod, Nathan David"],"institution":"The University of Texas Medical Branch at Galveston","degree_name":"Biochemistry and Molecular Biology (Doctoral)","degree_level":"Doctoral","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":null,"date_issued":"","date_published":null,"updated_at":"2026-07-24T05:50:56Z","subjects":["RNA biology, Transcription"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2152.3/11363","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Elrod, Nathan David"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2022-01-18T16:29:00Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2022-01-18T16:29:00Z"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Doctoral"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Biochemistry and Molecular Biology (Doctoral)"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["The University of Texas Medical Branch at Galveston"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["RNA biology, Transcription"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/2152.3/11363"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The Integrator complex is a 14-subunit protein complex that interacts with RNA polymerase II (RNAPII) in metazoans during transcription of multiple forms of coding and non-coding RNA. Originally described as being responsible for the 3’ cleavage and termination of uridine-rich small nuclear RNA, further roles have been discovered in the cleavage of long non-coding RNA, enhancer RNA, telomerase RNA, and coding messenger RNAs. While many studies have identified a presence at the transcriptional start site of mRNA, Integrator’s function at these mRNA has yet to be identified. In this study, we used multiple biochemical and sequencing techniques to elucidate a role for Integrator at these mRNA start sites. We found that the endonuclease activity of Integrator subunit 11 (IntS11) was responsible for the attenuation of mRNA through promoter-proximal termination of a large set of paused mRNAs. We also identified a novel interaction between IntS11 and CG7044 that could inhibit IntS11’s endonuclease activity."]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["THE ROLE OF INTEGRATOR SUBUNIT 11 IN PROMOTOR-PROXIMAL ATTENUATION OF PROTEIN CODING GENES"]}]}],"canonical_facts":{"dc:creator":["Elrod, Nathan David"],"dc:date.accessioned":["2022-01-18T16:29:00Z"],"dc:date.available":["2022-01-18T16:29:00Z"],"dc:description.abstract":["The Integrator complex is a 14-subunit protein complex that interacts with RNA polymerase II (RNAPII) in metazoans during transcription of multiple forms of coding and non-coding RNA. Originally described as being responsible for the 3’ cleavage and termination of uridine-rich small nuclear RNA, further roles have been discovered in the cleavage of long non-coding RNA, enhancer RNA, telomerase RNA, and coding messenger RNAs. While many studies have identified a presence at the transcriptional start site of mRNA, Integrator’s function at these mRNA has yet to be identified. In this study, we used multiple biochemical and sequencing techniques to elucidate a role for Integrator at these mRNA start sites. We found that the endonuclease activity of Integrator subunit 11 (IntS11) was responsible for the attenuation of mRNA through promoter-proximal termination of a large set of paused mRNAs. We also identified a novel interaction between IntS11 and CG7044 that could inhibit IntS11’s endonuclease activity."],"dc:format.mimetype":["application/pdf"],"dc:identifier.uri":["https://hdl.handle.net/2152.3/11363"],"dc:subject":["RNA biology, Transcription"],"dc:title":["THE ROLE OF INTEGRATOR SUBUNIT 11 IN PROMOTOR-PROXIMAL ATTENUATION OF PROTEIN CODING GENES"],"dc:type":["Thesis"],"thesis:degree_level":["Doctoral"],"thesis:degree_name":["Biochemistry and Molecular Biology (Doctoral)"],"thesis:institution_name":["The University of Texas Medical Branch at Galveston"]},"updated_at":"2026-07-24T05:50:56Z"}