Cleavage and polyadenylation specific factor 6 (Cpsf6) translocation is frequently reported in APLL, which is Acute Promyelocytic Leukemia (APL)-like acute myeloid leukemia (AML) and other types of blood diseases associated with abnormal hematopoiesis. CPSF6 is a subunit of the Cleavage Factor I mammalian (CFIm) complex, which regulates post-transcriptional alternative polyadenylation (APA) of pre-mRNAs. Previous research indicated that Cpsf6 plays an essential role in alternative polyadenylation, development, and tumorigenesis. However, the role of Cpsf6 in hematopoiesis is unknown. Given its roles in APA, development and cancer, we hypothesize that Cpsf6 plays a critical role in hematopoiesis and that its dysregulation may have significant impacts on this process. To test this, we used conditional knockout mouse models to examine the function of Cpsf6 in both embryonic and adult hematopoiesis. Our findings demonstrate that Cpsf6 is essential for embryonic hematopoiesis but is dispensable for maintaining adult hematopoietic homeostasis.
In addition to this primary work, I contributed to related exploratory studies on RARG fusion genes reported in APLL. My efforts laid the foundation for future work on RARG fusions in leukemia.
","abstract_html":"<p>Cleavage and polyadenylation specific factor 6 (<em>Cpsf6</em>) translocation is frequently reported in APLL, which is Acute Promyelocytic Leukemia (APL)-like acute myeloid leukemia (AML) and other types of blood diseases associated with abnormal hematopoiesis. CPSF6 is a subunit of the Cleavage Factor I mammalian (CFIm) complex, which regulates post-transcriptional alternative polyadenylation (APA) of pre-mRNAs. Previous research indicated that <em>Cpsf6</em> plays an essential role in alternative polyadenylation, development, and tumorigenesis. However, the role of <em>Cpsf6</em> in hematopoiesis is unknown. Given its roles in APA, development and cancer, we hypothesize that <em>Cpsf6</em> plays a critical role in hematopoiesis and that its dysregulation may have significant impacts on this process. To test this, we used conditional knockout mouse models to examine the function of <em>Cpsf6</em> in both embryonic and adult hematopoiesis. Our findings demonstrate that <em>Cpsf6</em> is essential for embryonic hematopoiesis but is dispensable for maintaining adult hematopoietic homeostasis.</p> <p>In addition to this primary work, I contributed to related exploratory studies on RARG fusion genes reported in APLL. My efforts laid the foundation for future work on RARG fusions in leukemia.</p>","abstract_has_math":false,"creators":["Ye, Fengxi","0009-0009-7833-8320
"],"institution":null,"degree_name":"Masters of Science (MS)","degree_level":"Thesis (MS)","degree_discipline":null,"degree_department":null,"school":null,"contributors":["M. James You","Pamela L. Wenzel","Angela H. Ting"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2025,"date_issued":"2025-08-01T07:00:00Z","date_published":"2025-08-01T07:00:00Z","updated_at":"2026-07-24T05:49:08Z","subjects":["Hematopoiesis","CPSF6","Leukemia","APL","Alternative Polyadenylation","Cell Biology","Developmental Biology","Genetics"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://digitalcommons.library.tmc.edu/utgsbs_dissertations/1469","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["M. James You","Pamela L. Wenzel","Angela H. Ting"]},{"key":"dc:creator","label":"Author","values":["Ye, Fengxi","0009-0009-7833-8320
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In addition to this primary work, I contributed to related exploratory studies on RARG fusion genes reported in APLL. My efforts laid the foundation for future work on RARG fusions in leukemia.
"]},{"key":"dc:title","label":"Title","values":["The Role of Cleavage and Polyadenylation Spcific Factor 6 (CPSF6) in Hematopoiesis"]}]}],"canonical_facts":{"dc:contributor":["M. James You","Pamela L. Wenzel","Angela H. Ting"],"dc:creator":["Ye, Fengxi","0009-0009-7833-8320
"],"dc:date.available":["2026-07-25T07:00:00Z"],"dc:description.abstract":["Cleavage and polyadenylation specific factor 6 (Cpsf6) translocation is frequently reported in APLL, which is Acute Promyelocytic Leukemia (APL)-like acute myeloid leukemia (AML) and other types of blood diseases associated with abnormal hematopoiesis. CPSF6 is a subunit of the Cleavage Factor I mammalian (CFIm) complex, which regulates post-transcriptional alternative polyadenylation (APA) of pre-mRNAs. Previous research indicated that Cpsf6 plays an essential role in alternative polyadenylation, development, and tumorigenesis. However, the role of Cpsf6 in hematopoiesis is unknown. Given its roles in APA, development and cancer, we hypothesize that Cpsf6 plays a critical role in hematopoiesis and that its dysregulation may have significant impacts on this process. To test this, we used conditional knockout mouse models to examine the function of Cpsf6 in both embryonic and adult hematopoiesis. Our findings demonstrate that Cpsf6 is essential for embryonic hematopoiesis but is dispensable for maintaining adult hematopoietic homeostasis.
In addition to this primary work, I contributed to related exploratory studies on RARG fusion genes reported in APLL. My efforts laid the foundation for future work on RARG fusions in leukemia.
"],"dc:identifier":["https://digitalcommons.library.tmc.edu/utgsbs_dissertations/1469"],"dc:subject":["Hematopoiesis","CPSF6","Leukemia","APL","Alternative Polyadenylation","Cell Biology","Developmental Biology","Genetics"],"dc:title":["The Role of Cleavage and Polyadenylation Spcific Factor 6 (CPSF6) in Hematopoiesis"],"thesis:degree_level":["Thesis (MS)"],"thesis:degree_name":["Masters of Science (MS)"]},"updated_at":"2026-07-24T05:49:08Z"}