Staphylococcus pseudintermedius is a Gram-positive bacterium significant because of its ability to cause costly and difficult to treat veterinary infections worldwide. It exhibits several similarities to Staphylococcus aureus, however, very little is known about its surface adhesins. Surface adhesins in S. aureus are significant contributors to pathogenesis. S. pseudintermedius encodes the surface protein SpsD, which contains characteristics of the microbial surface components recognizing adhesive matrix molecules family and confers attachment of the heterologous host Lactococcus lactis to fibronectin. This work has identified a centrally-located fibronectin binding domain in SpsD which binds the 30 kDa N-terminal domain of fibronectin with high affinity. The data indicate that a tandem β-zipper mechanism of binding may be taking place, and warrants further study into SpsD’s role in overall colonization of the host.
","abstract_html":"<p><em>Staphylococcus pseudintermedius</em> is a Gram-positive bacterium significant because of its ability to cause costly and difficult to treat veterinary infections worldwide. It exhibits several similarities to <em>Staphylococcus aureus</em>, however, very little is known about its surface adhesins. Surface adhesins in <em>S. aureus</em> are significant contributors to pathogenesis. <em>S. pseudintermedius</em> encodes the surface protein SpsD, which contains characteristics of the microbial surface components recognizing adhesive matrix molecules family and confers attachment of the heterologous host <em>Lactococcus lactis</em> to fibronectin. This work has identified a centrally-located fibronectin binding domain in SpsD which binds the 30 kDa N-terminal domain of fibronectin with high affinity. The data indicate that a tandem β-zipper mechanism of binding may be taking place, and warrants further study into SpsD’s role in overall colonization of the host.</p>","abstract_has_math":false,"creators":["Bordt, Andrea S."],"institution":null,"degree_name":"Masters of Science (MS)","degree_level":"Thesis (MS)","degree_discipline":null,"degree_department":null,"school":null,"contributors":["Dr. Magnus Hook","Dr. Steven Norris","Dr. Theresa Koehler"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2013,"date_issued":"2013-12-01T08:00:00Z","date_published":"2013-12-01T08:00:00Z","updated_at":"2026-07-24T05:48:47Z","subjects":["Staphylococcus pseudintermedius","SpsD","Fibronectin","Animal Diseases","Biochemistry","Biology","Medicine and Health Sciences"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://digitalcommons.library.tmc.edu/utgsbs_dissertations/412","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Dr. Magnus Hook","Dr. Steven Norris","Dr. Theresa Koehler"]},{"key":"dc:creator","label":"Author","values":["Bordt, Andrea S."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2013-12-13T08:00:00Z"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis (MS)"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Masters of Science (MS)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Staphylococcus pseudintermedius","SpsD","Fibronectin","Animal Diseases","Biochemistry","Biology","Medicine and Health Sciences"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://digitalcommons.library.tmc.edu/utgsbs_dissertations/412"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Staphylococcus pseudintermedius is a Gram-positive bacterium significant because of its ability to cause costly and difficult to treat veterinary infections worldwide. It exhibits several similarities to Staphylococcus aureus, however, very little is known about its surface adhesins. Surface adhesins in S. aureus are significant contributors to pathogenesis. S. pseudintermedius encodes the surface protein SpsD, which contains characteristics of the microbial surface components recognizing adhesive matrix molecules family and confers attachment of the heterologous host Lactococcus lactis to fibronectin. This work has identified a centrally-located fibronectin binding domain in SpsD which binds the 30 kDa N-terminal domain of fibronectin with high affinity. The data indicate that a tandem β-zipper mechanism of binding may be taking place, and warrants further study into SpsD’s role in overall colonization of the host.
"]},{"key":"dc:title","label":"Title","values":["The Staphylococcus Pseudintermedius Adhesin Spsd Contains A Central Fibronectin-Binding Domain"]}]}],"canonical_facts":{"dc:contributor":["Dr. Magnus Hook","Dr. Steven Norris","Dr. Theresa Koehler"],"dc:creator":["Bordt, Andrea S."],"dc:date.available":["2013-12-13T08:00:00Z"],"dc:description.abstract":["Staphylococcus pseudintermedius is a Gram-positive bacterium significant because of its ability to cause costly and difficult to treat veterinary infections worldwide. It exhibits several similarities to Staphylococcus aureus, however, very little is known about its surface adhesins. Surface adhesins in S. aureus are significant contributors to pathogenesis. S. pseudintermedius encodes the surface protein SpsD, which contains characteristics of the microbial surface components recognizing adhesive matrix molecules family and confers attachment of the heterologous host Lactococcus lactis to fibronectin. This work has identified a centrally-located fibronectin binding domain in SpsD which binds the 30 kDa N-terminal domain of fibronectin with high affinity. The data indicate that a tandem β-zipper mechanism of binding may be taking place, and warrants further study into SpsD’s role in overall colonization of the host.
"],"dc:identifier":["https://digitalcommons.library.tmc.edu/utgsbs_dissertations/412"],"dc:subject":["Staphylococcus pseudintermedius","SpsD","Fibronectin","Animal Diseases","Biochemistry","Biology","Medicine and Health Sciences"],"dc:title":["The Staphylococcus Pseudintermedius Adhesin Spsd Contains A Central Fibronectin-Binding Domain"],"thesis:degree_level":["Thesis (MS)"],"thesis:degree_name":["Masters of Science (MS)"]},"updated_at":"2026-07-24T05:48:47Z"}