{"id":{"repo_id":"uthm","oai_identifier":"oai:eprints.uthm.edu.my:91"},"canonical_url":"https://search.dev.ndltd.org/etd/uthm/oai:eprints.uthm.edu.my:91","repository":{"repo_id":"uthm","name":"Universiti Tun Hussein Onn Malaysia","base_url":"http://eprints.uthm.edu.my/cgi/oai2"},"display":{"title":"Antioxidant and anticholinesterase activities of centella asiatica phenolic extract-mediated gold nanoparticles","abstract":"In the present study, the green synthesis and optimization of gold nanoparticles (GNPs) using C. asiatica phenolic extract (CAP) were reported followed by the characterization with UV-vis, FTIR, Zeta potential, FESEM, EDX and XRD analyses. Total phenolic and flavonoid contents (TPC and TFC), antioxidant and anticholinesterase (anti-ChE) activities were also tested in vitro and Pearson correlation analysis was performed. The GNP synthesis using CAP was successfully accomplished under optimum conditions of reactants ratio of 1:1, 8 % CAP concentration, 0.5 mM HAuCl4 concentration and pH 9 at different temperatures of reaction medium (25 ℃, 40 ℃, 55 ℃ and 70 ℃). The results showed that the size range of GNPs was reduced from 6-40 nm at 25 ℃ to 6-30 nm at 40 ℃ and 55 ℃ while the sphericity of GNPs increased and the surface plasmon resonance (SPR) band became narrow. Although all GNP samples showed good antioxidant and anti-ChE activities (% I), the highest activity was recorded for the GNPs synthesized at 55 ℃, that is, 69.06 % and 76.72 % for ABTS and DPPH, and 71.71 % and 69.88 % for AChE and BChE, respectively, at the highest concentration of 500 μL sample. However, the maximum % I for ABTS and DPPH scavenging (79.76±1.01 and 75.51±1.54, respectively) was shown by ascorbic acid, and for AChE and BChE inhibition (75.18±2.29 and 79.94±1.11, respectively) by galanthamine. Pearson correlation analyses revealed a strong positive correlation between antioxidant activity and phenolic content as well as between anti-ChE and antioxidant activities for all GNP samples. The GNPs did not possess a significant cytotoxicity as shown by 20% mortality in brine shrimp lethality assay. The GNPs were haemocompatible as they induced 0.92 % haemolysis at the highest concentration (500 μL) in haemolysis assay compared to 99 % haemolysis by positive control. In conclusion, the results of this study show that CAP-GNPs possess good antioxidant and anticholinesterase activities, in addition to being non-cytotoxic and non-haemolytic, may be an option to further explore their therapeutic potential as antioxidant and cholinesterase inhibitors.","abstract_html":"In the present study, the green synthesis and optimization of gold nanoparticles (GNPs) using C. asiatica phenolic extract (CAP) were reported followed by the characterization with UV-vis, FTIR, Zeta potential, FESEM, EDX and XRD analyses. Total phenolic and flavonoid contents (TPC and TFC), antioxidant and anticholinesterase (anti-ChE) activities were also tested in vitro and Pearson correlation analysis was performed. The GNP synthesis using CAP was successfully accomplished under optimum conditions of reactants ratio of 1:1, 8 % CAP concentration, 0.5 mM HAuCl4 concentration and pH 9 at different temperatures of reaction medium (25 ℃, 40 ℃, 55 ℃ and 70 ℃). The results showed that the size range of GNPs was reduced from 6-40 nm at 25 ℃ to 6-30 nm at 40 ℃ and 55 ℃ while the sphericity of GNPs increased and the surface plasmon resonance (SPR) band became narrow. Although all GNP samples showed good antioxidant and anti-ChE activities (% I), the highest activity was recorded for the GNPs synthesized at 55 ℃, that is, 69.06 % and 76.72 % for ABTS and DPPH, and 71.71 % and 69.88 % for AChE and BChE, respectively, at the highest concentration of 500 μL sample. However, the maximum % I for ABTS and DPPH scavenging (79.76±1.01 and 75.51±1.54, respectively) was shown by ascorbic acid, and for AChE and BChE inhibition (75.18±2.29 and 79.94±1.11, respectively) by galanthamine. Pearson correlation analyses revealed a strong positive correlation between antioxidant activity and phenolic content as well as between anti-ChE and antioxidant activities for all GNP samples. The GNPs did not possess a significant cytotoxicity as shown by 20% mortality in brine shrimp lethality assay. The GNPs were haemocompatible as they induced 0.92 % haemolysis at the highest concentration (500 μL) in haemolysis assay compared to 99 % haemolysis by positive control. In conclusion, the results of this study show that CAP-GNPs possess good antioxidant and anticholinesterase activities, in addition to being non-cytotoxic and non-haemolytic, may be an option to further explore their therapeutic potential as antioxidant and cholinesterase inhibitors.","abstract_has_math":false,"creators":["Latif, Muhammad Sohail"],"institution":"Universiti Tun Hussein Onn Malaysia","degree_name":"phd","degree_level":"doctoral","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2019,"date_issued":"2019-07","date_published":"2019-07","updated_at":"2026-07-24T05:47:35Z","subjects":["TX341-641 Nutrition. Foods and food supply"],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Latif, Muhammad Sohail"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2019-07"]},{"key":"dc:date.issued","label":"Date","values":["2019-07"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["Fakulti Sains Gunaan dan Teknolgi"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["Universiti Tun Hussein Onn Malaysia"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["http://eprints.uthm.edu.my/91/"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["doctoral"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["phd"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["TX341-641 Nutrition. Foods and food supply"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://eprints.uthm.edu.my/91/1/24p%20MUHAMMAD%20SOHAIL%20LATIF.pdf","http://eprints.uthm.edu.my/91/2/MUHAMMAD%20SOHAIL%20LATIF%20WATERMARK.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["In the present study, the green synthesis and optimization of gold nanoparticles (GNPs) using C. asiatica phenolic extract (CAP) were reported followed by the characterization with UV-vis, FTIR, Zeta potential, FESEM, EDX and XRD analyses. Total phenolic and flavonoid contents (TPC and TFC), antioxidant and anticholinesterase (anti-ChE) activities were also tested in vitro and Pearson correlation analysis was performed. The GNP synthesis using CAP was successfully accomplished under optimum conditions of reactants ratio of 1:1, 8 % CAP concentration, 0.5 mM HAuCl4 concentration and pH 9 at different temperatures of reaction medium (25 ℃, 40 ℃, 55 ℃ and 70 ℃). The results showed that the size range of GNPs was reduced from 6-40 nm at 25 ℃ to 6-30 nm at 40 ℃ and 55 ℃ while the sphericity of GNPs increased and the surface plasmon resonance (SPR) band became narrow. Although all GNP samples showed good antioxidant and anti-ChE activities (% I), the highest activity was recorded for the GNPs synthesized at 55 ℃, that is, 69.06 % and 76.72 % for ABTS and DPPH, and 71.71 % and 69.88 % for AChE and BChE, respectively, at the highest concentration of 500 μL sample. However, the maximum % I for ABTS and DPPH scavenging (79.76±1.01 and 75.51±1.54, respectively) was shown by ascorbic acid, and for AChE and BChE inhibition (75.18±2.29 and 79.94±1.11, respectively) by galanthamine. Pearson correlation analyses revealed a strong positive correlation between antioxidant activity and phenolic content as well as between anti-ChE and antioxidant activities for all GNP samples. The GNPs did not possess a significant cytotoxicity as shown by 20% mortality in brine shrimp lethality assay. The GNPs were haemocompatible as they induced 0.92 % haemolysis at the highest concentration (500 μL) in haemolysis assay compared to 99 % haemolysis by positive control. In conclusion, the results of this study show that CAP-GNPs possess good antioxidant and anticholinesterase activities, in addition to being non-cytotoxic and non-haemolytic, may be an option to further explore their therapeutic potential as antioxidant and cholinesterase inhibitors."]},{"key":"dc:format","label":"Dc Format","values":["text"]},{"key":"dc:title","label":"Title","values":["Antioxidant and anticholinesterase activities of centella asiatica phenolic extract-mediated gold nanoparticles"]}]}],"canonical_facts":{"dc:creator":["Latif, Muhammad Sohail"],"dc:date":["2019-07"],"dc:date.issued":["2019-07"],"dc:description.abstract":["In the present study, the green synthesis and optimization of gold nanoparticles (GNPs) using C. asiatica phenolic extract (CAP) were reported followed by the characterization with UV-vis, FTIR, Zeta potential, FESEM, EDX and XRD analyses. Total phenolic and flavonoid contents (TPC and TFC), antioxidant and anticholinesterase (anti-ChE) activities were also tested in vitro and Pearson correlation analysis was performed. The GNP synthesis using CAP was successfully accomplished under optimum conditions of reactants ratio of 1:1, 8 % CAP concentration, 0.5 mM HAuCl4 concentration and pH 9 at different temperatures of reaction medium (25 ℃, 40 ℃, 55 ℃ and 70 ℃). The results showed that the size range of GNPs was reduced from 6-40 nm at 25 ℃ to 6-30 nm at 40 ℃ and 55 ℃ while the sphericity of GNPs increased and the surface plasmon resonance (SPR) band became narrow. Although all GNP samples showed good antioxidant and anti-ChE activities (% I), the highest activity was recorded for the GNPs synthesized at 55 ℃, that is, 69.06 % and 76.72 % for ABTS and DPPH, and 71.71 % and 69.88 % for AChE and BChE, respectively, at the highest concentration of 500 μL sample. However, the maximum % I for ABTS and DPPH scavenging (79.76±1.01 and 75.51±1.54, respectively) was shown by ascorbic acid, and for AChE and BChE inhibition (75.18±2.29 and 79.94±1.11, respectively) by galanthamine. Pearson correlation analyses revealed a strong positive correlation between antioxidant activity and phenolic content as well as between anti-ChE and antioxidant activities for all GNP samples. The GNPs did not possess a significant cytotoxicity as shown by 20% mortality in brine shrimp lethality assay. The GNPs were haemocompatible as they induced 0.92 % haemolysis at the highest concentration (500 μL) in haemolysis assay compared to 99 % haemolysis by positive control. In conclusion, the results of this study show that CAP-GNPs possess good antioxidant and anticholinesterase activities, in addition to being non-cytotoxic and non-haemolytic, may be an option to further explore their therapeutic potential as antioxidant and cholinesterase inhibitors."],"dc:format":["text"],"dc:identifier.uri":["http://eprints.uthm.edu.my/91/1/24p%20MUHAMMAD%20SOHAIL%20LATIF.pdf","http://eprints.uthm.edu.my/91/2/MUHAMMAD%20SOHAIL%20LATIF%20WATERMARK.pdf"],"dc:language":["en"],"dc:publisher.department":["Fakulti Sains Gunaan dan Teknolgi"],"dc:publisher.institution":["Universiti Tun Hussein Onn Malaysia"],"dc:relation.isreferencedby":["http://eprints.uthm.edu.my/91/"],"dc:subject":["TX341-641 Nutrition. Foods and food supply"],"dc:title":["Antioxidant and anticholinesterase activities of centella asiatica phenolic extract-mediated gold nanoparticles"],"dc:type":["Thesis"],"dc:type.qualificationlevel":["doctoral"],"dc:type.qualificationname":["phd"]},"updated_at":"2026-07-24T05:47:35Z"}