Whether 5'-triphosphate-single-stranded-RNA (5 '-ppp-ssRNA) is immunostimulatory was controversial in the literature. In order to clarify the immunostimulatory effect of 5'-ppp-ssRNA, we synthesized ssRNAs and tested the IFNp (interferon-beta) level and cellular toxicity of ssRNAs in SKOV3 cells. In this work, we confirmed that 5'-ppp-ssRNA (~ 60nt) was immunostimulatory and toxic in SKOV3 cells. 5'-ppp-ssRNA (~ 60nt) led to IFN-P induction and apoptosis in SKOV3 cells. Longer 5' ppp- ssRNA tended to have a higher immunostimulatory effect and cellular toxicity than shorter 5'-ppp-ssRNA. 5'-ppp-ssRNA was more immunostimulatory and toxic than 5'- 0H-ssRNA. If the incubation time of ssRNAs increased, the toxicity of ssRNAs would increase. Higher concentrations of ssRNA tended to be more toxic than lower concentrations of ssRNA. RIG-I (retinoic acid-inducible gene 1) plays a role in the recognition of 5' -ppp-ssRNA. This work provides a guideline for the design of immunostimulatory RN As which can be applied in cancer therapy.
","abstract_html":"<p>Whether 5'-triphosphate-single-stranded-RNA (5 '-ppp-ssRNA) is immunostimulatory was controversial in the literature. In order to clarify the immunostimulatory effect of 5'-ppp-ssRNA, we synthesized ssRNAs and tested the IFNp (interferon-beta) level and cellular toxicity of ssRNAs in SKOV3 cells. In this work, we confirmed that 5'-ppp-ssRNA (~ 60nt) was immunostimulatory and toxic in SKOV3 cells. 5'-ppp-ssRNA (~ 60nt) led to IFN-P induction and apoptosis in SKOV3 cells. Longer 5' ppp- ssRNA tended to have a higher immunostimulatory effect and cellular toxicity than shorter 5'-ppp-ssRNA. 5'-ppp-ssRNA was more immunostimulatory and toxic than 5'- 0H-ssRNA. If the incubation time of ssRNAs increased, the toxicity of ssRNAs would increase. Higher concentrations of ssRNA tended to be more toxic than lower concentrations of ssRNA. RIG-I (retinoic acid-inducible gene 1) plays a role in the recognition of 5' -ppp-ssRNA. This work provides a guideline for the design of immunostimulatory RN As which can be applied in cancer therapy.</p>","abstract_has_math":false,"creators":["Teng, Chengwen"],"institution":null,"degree_name":"Master of Science (MS)","degree_level":"Masters Thesis","degree_discipline":"Biological Sciences","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2012,"date_issued":"2012-08-01T07:00:00Z","date_published":"2012-08-01T07:00:00Z","updated_at":"2026-07-24T05:45:06Z","subjects":["Biology","Life Sciences"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://aquila.usm.edu/masters_theses/564","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Teng, Chengwen"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2018-11-27T08:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biological Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Masters Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science (MS)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology","Life Sciences"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://aquila.usm.edu/masters_theses/564"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Whether 5'-triphosphate-single-stranded-RNA (5 '-ppp-ssRNA) is immunostimulatory was controversial in the literature. In order to clarify the immunostimulatory effect of 5'-ppp-ssRNA, we synthesized ssRNAs and tested the IFNp (interferon-beta) level and cellular toxicity of ssRNAs in SKOV3 cells. In this work, we confirmed that 5'-ppp-ssRNA (~ 60nt) was immunostimulatory and toxic in SKOV3 cells. 5'-ppp-ssRNA (~ 60nt) led to IFN-P induction and apoptosis in SKOV3 cells. Longer 5' ppp- ssRNA tended to have a higher immunostimulatory effect and cellular toxicity than shorter 5'-ppp-ssRNA. 5'-ppp-ssRNA was more immunostimulatory and toxic than 5'- 0H-ssRNA. If the incubation time of ssRNAs increased, the toxicity of ssRNAs would increase. Higher concentrations of ssRNA tended to be more toxic than lower concentrations of ssRNA. RIG-I (retinoic acid-inducible gene 1) plays a role in the recognition of 5' -ppp-ssRNA. This work provides a guideline for the design of immunostimulatory RN As which can be applied in cancer therapy.
"]},{"key":"dc:title","label":"Title","values":["Immunostimulatory and Cellular Toxic Effects of Single-Stranded RNA On Cancer Cells"]}]}],"canonical_facts":{"dc:creator":["Teng, Chengwen"],"dc:date.available":["2018-11-27T08:00:00Z"],"dc:description.abstract":["Whether 5'-triphosphate-single-stranded-RNA (5 '-ppp-ssRNA) is immunostimulatory was controversial in the literature. In order to clarify the immunostimulatory effect of 5'-ppp-ssRNA, we synthesized ssRNAs and tested the IFNp (interferon-beta) level and cellular toxicity of ssRNAs in SKOV3 cells. In this work, we confirmed that 5'-ppp-ssRNA (~ 60nt) was immunostimulatory and toxic in SKOV3 cells. 5'-ppp-ssRNA (~ 60nt) led to IFN-P induction and apoptosis in SKOV3 cells. Longer 5' ppp- ssRNA tended to have a higher immunostimulatory effect and cellular toxicity than shorter 5'-ppp-ssRNA. 5'-ppp-ssRNA was more immunostimulatory and toxic than 5'- 0H-ssRNA. If the incubation time of ssRNAs increased, the toxicity of ssRNAs would increase. Higher concentrations of ssRNA tended to be more toxic than lower concentrations of ssRNA. RIG-I (retinoic acid-inducible gene 1) plays a role in the recognition of 5' -ppp-ssRNA. This work provides a guideline for the design of immunostimulatory RN As which can be applied in cancer therapy.
"],"dc:identifier":["https://aquila.usm.edu/masters_theses/564"],"dc:subject":["Biology","Life Sciences"],"dc:title":["Immunostimulatory and Cellular Toxic Effects of Single-Stranded RNA On Cancer Cells"],"thesis:degree_discipline":["Biological Sciences"],"thesis:degree_level":["Masters Thesis"],"thesis:degree_name":["Master of Science (MS)"]},"updated_at":"2026-07-24T05:45:06Z"}