Because of the high occurrence of marijuana usage in the workplace and in driving under the influence cases, it is important to test for both delta-9-THC and delta-9-THCA in order to determine current and recent usage. The laboratory preparation and analysis of delta-9-THC and delta-9-THCA in urine should be as cost and time efficient as possible. Current extraction of delta-9-THC and delta-9-THCA from urine requires two separate techniques for each. Because delta-9-THC is nonacidic, while delta-9-THCA is acidic, the extractions involve two separate pH levels, while GC/MS analysis requires two separate methods. This separation requires a great deal of time and materials. This experiment focused on eliminating the need for two separate extractions and analyses of delta-9-THC and delta-9-THCA. Both were subjected to simultaneous acidic extraction with hexane:ethyl acetate (4: 1). An F-ratio test determined that the dual extraction of delta-9-THC and delta-9-THCA gave concentrations that had no significant differences. The F-ratio for delta-9-THC concentrations was 0.191, while the F-ratio for delta-9-THCA concentrations was 0.021. Both fell under the critical value was 5.08 for a 0.01 level of significance. This showed that the concentrations of delta-9-of F for the analysis of variance from the table which THC and delta-9-THCA calculated for each sample's three aliquots were all significantly similar.
","abstract_html":"<p>Because of the high occurrence of marijuana usage in the workplace and in driving under the influence cases, it is important to test for both delta-9-THC and delta-9-THCA in order to determine current and recent usage. The laboratory preparation and analysis of delta-9-THC and delta-9-THCA in urine should be as cost and time efficient as possible. Current extraction of delta-9-THC and delta-9-THCA from urine requires two separate techniques for each. Because delta-9-THC is nonacidic, while delta-9-THCA is acidic, the extractions involve two separate pH levels, while GC/MS analysis requires two separate methods. This separation requires a great deal of time and materials. This experiment focused on eliminating the need for two separate extractions and analyses of delta-9-THC and delta-9-THCA. Both were subjected to simultaneous acidic extraction with hexane:ethyl acetate (4: 1). An F-ratio test determined that the dual extraction of delta-9-THC and delta-9-THCA gave concentrations that had no significant differences. The F-ratio for delta-9-THC concentrations was 0.191, while the F-ratio for delta-9-THCA concentrations was 0.021. Both fell under the critical value was 5.08 for a 0.01 level of significance. This showed that the concentrations of delta-9-of F for the analysis of variance from the table which THC and delta-9-THCA calculated for each sample's three aliquots were all significantly similar.</p>","abstract_has_math":false,"creators":["Tanner, Meghan Ann"],"institution":null,"degree_name":"Master of Science (MS)","degree_level":"Masters Thesis","degree_discipline":"Criminal Justice","degree_department":null,"school":null,"contributors":["Thomas Pittman","Dean Bertram","Kuppareddi Balamurugan"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2012,"date_issued":"2012-08-01T07:00:00Z","date_published":"2012-08-01T07:00:00Z","updated_at":"2026-07-24T05:45:06Z","subjects":["Forensic Science and Technology","Legal Studies","Social and Behavioral Sciences"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://aquila.usm.edu/masters_theses/562","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Thomas Pittman","Dean Bertram","Kuppareddi Balamurugan"]},{"key":"dc:creator","label":"Author","values":["Tanner, Meghan Ann"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2018-11-27T08:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Criminal Justice"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Masters Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science (MS)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Forensic Science and Technology","Legal Studies","Social and Behavioral Sciences"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://aquila.usm.edu/masters_theses/562"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Because of the high occurrence of marijuana usage in the workplace and in driving under the influence cases, it is important to test for both delta-9-THC and delta-9-THCA in order to determine current and recent usage. The laboratory preparation and analysis of delta-9-THC and delta-9-THCA in urine should be as cost and time efficient as possible. Current extraction of delta-9-THC and delta-9-THCA from urine requires two separate techniques for each. Because delta-9-THC is nonacidic, while delta-9-THCA is acidic, the extractions involve two separate pH levels, while GC/MS analysis requires two separate methods. This separation requires a great deal of time and materials. This experiment focused on eliminating the need for two separate extractions and analyses of delta-9-THC and delta-9-THCA. Both were subjected to simultaneous acidic extraction with hexane:ethyl acetate (4: 1). An F-ratio test determined that the dual extraction of delta-9-THC and delta-9-THCA gave concentrations that had no significant differences. The F-ratio for delta-9-THC concentrations was 0.191, while the F-ratio for delta-9-THCA concentrations was 0.021. Both fell under the critical value was 5.08 for a 0.01 level of significance. This showed that the concentrations of delta-9-of F for the analysis of variance from the table which THC and delta-9-THCA calculated for each sample's three aliquots were all significantly similar.
"]},{"key":"dc:title","label":"Title","values":["Single Liquid-Liquid Extraction of Delta-9-Tetrahydrocannabinol and 11-Nor-Carboxy-Delta-9-Tetrahydrocannabinol at an Acidic pH"]}]}],"canonical_facts":{"dc:contributor":["Thomas Pittman","Dean Bertram","Kuppareddi Balamurugan"],"dc:creator":["Tanner, Meghan Ann"],"dc:date.available":["2018-11-27T08:00:00Z"],"dc:description.abstract":["Because of the high occurrence of marijuana usage in the workplace and in driving under the influence cases, it is important to test for both delta-9-THC and delta-9-THCA in order to determine current and recent usage. The laboratory preparation and analysis of delta-9-THC and delta-9-THCA in urine should be as cost and time efficient as possible. Current extraction of delta-9-THC and delta-9-THCA from urine requires two separate techniques for each. Because delta-9-THC is nonacidic, while delta-9-THCA is acidic, the extractions involve two separate pH levels, while GC/MS analysis requires two separate methods. This separation requires a great deal of time and materials. This experiment focused on eliminating the need for two separate extractions and analyses of delta-9-THC and delta-9-THCA. Both were subjected to simultaneous acidic extraction with hexane:ethyl acetate (4: 1). An F-ratio test determined that the dual extraction of delta-9-THC and delta-9-THCA gave concentrations that had no significant differences. The F-ratio for delta-9-THC concentrations was 0.191, while the F-ratio for delta-9-THCA concentrations was 0.021. Both fell under the critical value was 5.08 for a 0.01 level of significance. This showed that the concentrations of delta-9-of F for the analysis of variance from the table which THC and delta-9-THCA calculated for each sample's three aliquots were all significantly similar.
"],"dc:identifier":["https://aquila.usm.edu/masters_theses/562"],"dc:subject":["Forensic Science and Technology","Legal Studies","Social and Behavioral Sciences"],"dc:title":["Single Liquid-Liquid Extraction of Delta-9-Tetrahydrocannabinol and 11-Nor-Carboxy-Delta-9-Tetrahydrocannabinol at an Acidic pH"],"thesis:degree_discipline":["Criminal Justice"],"thesis:degree_level":["Masters Thesis"],"thesis:degree_name":["Master of Science (MS)"]},"updated_at":"2026-07-24T05:45:06Z"}