The effect of pesticides on Apis mellifera mortality as well as their effect on the transcriptional regulation of antioxidant genes was the aim of this study. Unlike previous studies, the work here shows the combined effects of pesticides imidacloprid and coumaphos on worker bees at levels likely encountered by workers using both toxological and molecular analysis. Bee brood were collected from hives and incubated until hatching. The 1 day old bees were then segregated and subjected to varying concentrations of coumaphos and imidacloprid both independently and in concert. Workers from each treatment group were removed and stored in RNA-later until they were used for molecular analysis. The 10 antioxidant genes monitored here comprised of both primary and secondary antioxidants. Four of the secondary antioxidants used were seleno-like genes found in the genome. These showed differential expression throughout the bee’s development as well in the presence of pesticides.
","abstract_html":"<p>The effect of pesticides on <em>Apis mellifera </em>mortality as well as their effect on the transcriptional regulation of antioxidant genes was the aim of this study. Unlike previous studies, the work here shows the combined effects of pesticides imidacloprid and coumaphos on worker bees at levels likely encountered by workers using both toxological and molecular analysis. Bee brood were collected from hives and incubated until hatching. The 1 day old bees were then segregated and subjected to varying concentrations of coumaphos and imidacloprid both independently and in concert. Workers from each treatment group were removed and stored in RNA-later until they were used for molecular analysis. The 10 antioxidant genes monitored here comprised of both primary and secondary antioxidants. Four of the secondary antioxidants used were seleno-like genes found in the genome. These showed differential expression throughout the bee’s development as well in the presence of pesticides.</p>","abstract_has_math":false,"creators":["Rinderer, Nicholas"],"institution":null,"degree_name":"Master of Science (MS)","degree_level":"Masters Thesis","degree_discipline":null,"degree_department":null,"school":null,"contributors":["Shahid Karim","Alex Flynt","Yanlin Guo"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2018,"date_issued":"2018-12-01T08:00:00Z","date_published":"2018-12-01T08:00:00Z","updated_at":"2026-07-24T05:44:50Z","subjects":["Apis mellifera","antioxidants","selenoproteins"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://aquila.usm.edu/masters_theses/586","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Shahid Karim","Alex Flynt","Yanlin Guo"]},{"key":"dc:creator","label":"Author","values":["Rinderer, Nicholas"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2019-06-07T07:00:00Z"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Masters Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science (MS)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Apis mellifera","antioxidants","selenoproteins"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://aquila.usm.edu/masters_theses/586"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The effect of pesticides on Apis mellifera mortality as well as their effect on the transcriptional regulation of antioxidant genes was the aim of this study. Unlike previous studies, the work here shows the combined effects of pesticides imidacloprid and coumaphos on worker bees at levels likely encountered by workers using both toxological and molecular analysis. Bee brood were collected from hives and incubated until hatching. The 1 day old bees were then segregated and subjected to varying concentrations of coumaphos and imidacloprid both independently and in concert. Workers from each treatment group were removed and stored in RNA-later until they were used for molecular analysis. The 10 antioxidant genes monitored here comprised of both primary and secondary antioxidants. Four of the secondary antioxidants used were seleno-like genes found in the genome. These showed differential expression throughout the bee’s development as well in the presence of pesticides.
"]},{"key":"dc:title","label":"Title","values":["Antioxidant Genes in Apis mellifera, Their Implication in Pesticide Detoxification"]}]}],"canonical_facts":{"dc:contributor":["Shahid Karim","Alex Flynt","Yanlin Guo"],"dc:creator":["Rinderer, Nicholas"],"dc:date.available":["2019-06-07T07:00:00Z"],"dc:description.abstract":["The effect of pesticides on Apis mellifera mortality as well as their effect on the transcriptional regulation of antioxidant genes was the aim of this study. Unlike previous studies, the work here shows the combined effects of pesticides imidacloprid and coumaphos on worker bees at levels likely encountered by workers using both toxological and molecular analysis. Bee brood were collected from hives and incubated until hatching. The 1 day old bees were then segregated and subjected to varying concentrations of coumaphos and imidacloprid both independently and in concert. Workers from each treatment group were removed and stored in RNA-later until they were used for molecular analysis. The 10 antioxidant genes monitored here comprised of both primary and secondary antioxidants. Four of the secondary antioxidants used were seleno-like genes found in the genome. These showed differential expression throughout the bee’s development as well in the presence of pesticides.
"],"dc:identifier":["https://aquila.usm.edu/masters_theses/586"],"dc:subject":["Apis mellifera","antioxidants","selenoproteins"],"dc:title":["Antioxidant Genes in Apis mellifera, Their Implication in Pesticide Detoxification"],"thesis:degree_level":["Masters Thesis"],"thesis:degree_name":["Master of Science (MS)"]},"updated_at":"2026-07-24T05:44:50Z"}