{"id":{"repo_id":"uoit","oai_identifier":"oai:ontariotechu.scholaris.ca:10155/833"},"canonical_url":"https://search.dev.ndltd.org/etd/uoit/oai:ontariotechu.scholaris.ca:10155/833","repository":{"repo_id":"uoit","name":"Ontario Institute of Technology","base_url":"https://ontariotechu.scholaris.ca/server/oai/request"},"display":{"title":"The transcription factor, fixK, plays a central role in carbon source metabolism and cellulose synthesis in Komagataeibacter species","abstract":"Komagataeibacter xylinus ATCC 53582 and K. hansenii ATCC 23769 are model bacterial cellulose producers. This thesis investigated both carbon source utilization and the role that a Crp/Fnr transcription factor, FixK, plays in bacterial cellulose biosynthesis. Extracellular proteins secreted by Komagataeibacter were assayed for degradative activity against plant cell wall compounds. Mutagenesis of fixK, previously associated with cellulose regulation, revealed its role in cellular metabolism. Growth, pH, gluconic acid, and cellulose yield were measured in static and agitated cultures grown in the presence of glucose, fructose or sucrose. BcsZ (formerly CmcAx) was found to be carbon-source regulated. K. hansenii was dependent on fixK for growth in fructose and sucrose, but not in glucose. Mutation of fixK abolished cellulose production in K. hansenii, but limited production in K. xylinus. Altogether, this study improves our understanding of carbon source utilization and bacterial cellulose synthesis in Komagataeibacter species.","abstract_html":"Komagataeibacter xylinus ATCC 53582 and K. hansenii ATCC 23769 are model bacterial cellulose producers. This thesis investigated both carbon source utilization and the role that a Crp/Fnr transcription factor, FixK, plays in bacterial cellulose biosynthesis. Extracellular proteins secreted by Komagataeibacter were assayed for degradative activity against plant cell wall compounds. Mutagenesis of fixK, previously associated with cellulose regulation, revealed its role in cellular metabolism. Growth, pH, gluconic acid, and cellulose yield were measured in static and agitated cultures grown in the presence of glucose, fructose or sucrose. BcsZ (formerly CmcAx) was found to be carbon-source regulated. K. hansenii was dependent on fixK for growth in fructose and sucrose, but not in glucose. Mutation of fixK abolished cellulose production in K. hansenii, but limited production in K. xylinus. Altogether, this study improves our understanding of carbon source utilization and bacterial cellulose synthesis in Komagataeibacter species.","abstract_has_math":false,"creators":["Varley, Andrew James"],"institution":"University of Ontario Institute of Technology","degree_name":"Master of Science (MSc)","degree_level":null,"degree_discipline":"Applied Bioscience","degree_department":null,"school":null,"contributors":[],"advisors":["Strap, Janice L."],"committee_chairs":[],"committee_members":[],"year":2017,"date_issued":"2017-07-01","date_published":"2017-07-01","updated_at":"2026-07-24T05:35:28Z","subjects":["Komagataeibacter xylinus","Komagataeibacter hansenii","fixK","Bacterial cellulose","Carbon source"],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/10155/833","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Strap, Janice L."]},{"key":"dc:creator","label":"Author","values":["Varley, Andrew James"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2017-11-16T14:18:36Z","2022-03-29T17:34:00Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2017-11-16T14:18:36Z","2022-03-29T17:34:00Z"]},{"key":"dc:date.issued","label":"Date","values":["2017-07-01"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Applied Bioscience"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science (MSc)"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Ontario Institute of Technology"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Komagataeibacter xylinus","Komagataeibacter hansenii","fixK","Bacterial cellulose","Carbon source"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/10155/833"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Komagataeibacter xylinus ATCC 53582 and K. hansenii ATCC 23769 are model bacterial cellulose producers. This thesis investigated both carbon source utilization and the role that a Crp/Fnr transcription factor, FixK, plays in bacterial cellulose biosynthesis. Extracellular proteins secreted by Komagataeibacter were assayed for degradative activity against plant cell wall compounds. Mutagenesis of fixK, previously associated with cellulose regulation, revealed its role in cellular metabolism. Growth, pH, gluconic acid, and cellulose yield were measured in static and agitated cultures grown in the presence of glucose, fructose or sucrose. BcsZ (formerly CmcAx) was found to be carbon-source regulated. K. hansenii was dependent on fixK for growth in fructose and sucrose, but not in glucose. Mutation of fixK abolished cellulose production in K. hansenii, but limited production in K. xylinus. Altogether, this study improves our understanding of carbon source utilization and bacterial cellulose synthesis in Komagataeibacter species."]},{"key":"dc:title","label":"Title","values":["The transcription factor, fixK, plays a central role in carbon source metabolism and cellulose synthesis in Komagataeibacter species"]}]}],"canonical_facts":{"dc:contributor.advisor":["Strap, Janice L."],"dc:creator":["Varley, Andrew James"],"dc:date.accessioned":["2017-11-16T14:18:36Z","2022-03-29T17:34:00Z"],"dc:date.available":["2017-11-16T14:18:36Z","2022-03-29T17:34:00Z"],"dc:date.issued":["2017-07-01"],"dc:description.abstract":["Komagataeibacter xylinus ATCC 53582 and K. hansenii ATCC 23769 are model bacterial cellulose producers. This thesis investigated both carbon source utilization and the role that a Crp/Fnr transcription factor, FixK, plays in bacterial cellulose biosynthesis. Extracellular proteins secreted by Komagataeibacter were assayed for degradative activity against plant cell wall compounds. Mutagenesis of fixK, previously associated with cellulose regulation, revealed its role in cellular metabolism. Growth, pH, gluconic acid, and cellulose yield were measured in static and agitated cultures grown in the presence of glucose, fructose or sucrose. BcsZ (formerly CmcAx) was found to be carbon-source regulated. K. hansenii was dependent on fixK for growth in fructose and sucrose, but not in glucose. Mutation of fixK abolished cellulose production in K. hansenii, but limited production in K. xylinus. Altogether, this study improves our understanding of carbon source utilization and bacterial cellulose synthesis in Komagataeibacter species."],"dc:identifier.uri":["https://hdl.handle.net/10155/833"],"dc:language.iso":["en"],"dc:subject":["Komagataeibacter xylinus","Komagataeibacter hansenii","fixK","Bacterial cellulose","Carbon source"],"dc:title":["The transcription factor, fixK, plays a central role in carbon source metabolism and cellulose synthesis in Komagataeibacter species"],"dc:type":["Thesis"],"thesis:degree_discipline":["Applied Bioscience"],"thesis:degree_name":["Master of Science (MSc)"],"thesis:institution_name":["University of Ontario Institute of Technology"]},"updated_at":"2026-07-24T05:35:28Z"}