{"id":{"repo_id":"uoit","oai_identifier":"oai:ontariotechu.scholaris.ca:10155/728"},"canonical_url":"https://search.dev.ndltd.org/etd/uoit/oai:ontariotechu.scholaris.ca:10155/728","repository":{"repo_id":"uoit","name":"Ontario Institute of Technology","base_url":"https://ontariotechu.scholaris.ca/server/oai/request"},"display":{"title":"Examining effects of milk ferment components on endothelial cell signaling in pro-inflammatory pathways","abstract":"Atherosclerosis is a complex, multifactorial, inflammatory disease involving cholesterol, endothelial cells, smooth muscle cells, macrophages and platelets. Atherosclerosis, and the associated cardiovascular diseases it contributes to, has been a growing health care and economic concern over the last 50 years. Bioactive peptides found in fermented milk have been shown to have cardio and athero-protective effects, but their mechanism has not been fully elucidated. The objective of this study was to test individual components of milk fermentation and macrophage conditioning in order to determine how fermented milk bioactive peptides best exert their effects. This study shows that protein secretions from bacteria, whole and fermented milk ferments and supernatants from macrophages conditioned with fermented milk do not down-regulate inflammatory activity in cultured endothelial cells, however moderate anti-inflammatory nitric oxide (NO) production by cultured endothelial cells was seen with peptides derived from the milk β -casein sequence, warranting further study.","abstract_html":"Atherosclerosis is a complex, multifactorial, inflammatory disease involving cholesterol, endothelial cells, smooth muscle cells, macrophages and platelets. Atherosclerosis, and the associated cardiovascular diseases it contributes to, has been a growing health care and economic concern over the last 50 years. Bioactive peptides found in fermented milk have been shown to have cardio and athero-protective effects, but their mechanism has not been fully elucidated. The objective of this study was to test individual components of milk fermentation and macrophage conditioning in order to determine how fermented milk bioactive peptides best exert their effects. This study shows that protein secretions from bacteria, whole and fermented milk ferments and supernatants from macrophages conditioned with fermented milk do not down-regulate inflammatory activity in cultured endothelial cells, however moderate anti-inflammatory nitric oxide (NO) production by cultured endothelial cells was seen with peptides derived from the milk β -casein sequence, warranting further study.","abstract_has_math":false,"creators":["Dobby, Kyle R.E."],"institution":"University of Ontario Institute of Technology","degree_name":"Master of Science (MSc)","degree_level":null,"degree_discipline":"Applied Bioscience","degree_department":null,"school":null,"contributors":[],"advisors":["Jones-Taggart, Holly"],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-08-01","date_published":"2015-08-01","updated_at":"2026-07-24T05:35:39Z","subjects":["Atherosclerosis","Cardiovascular disease","Bioactive peptides","Fermented milk","Endothelial cells"],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/10155/728","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Jones-Taggart, Holly"]},{"key":"dc:creator","label":"Author","values":["Dobby, Kyle R.E."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2017-03-20T13:35:08Z","2022-03-29T17:39:20Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2017-03-20T13:35:08Z","2022-03-29T17:39:20Z"]},{"key":"dc:date.issued","label":"Date","values":["2015-08-01"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Applied Bioscience"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science (MSc)"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Ontario Institute of Technology"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Atherosclerosis","Cardiovascular disease","Bioactive peptides","Fermented milk","Endothelial cells"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/10155/728"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Atherosclerosis is a complex, multifactorial, inflammatory disease involving cholesterol, endothelial cells, smooth muscle cells, macrophages and platelets. Atherosclerosis, and the associated cardiovascular diseases it contributes to, has been a growing health care and economic concern over the last 50 years. Bioactive peptides found in fermented milk have been shown to have cardio and athero-protective effects, but their mechanism has not been fully elucidated. The objective of this study was to test individual components of milk fermentation and macrophage conditioning in order to determine how fermented milk bioactive peptides best exert their effects. This study shows that protein secretions from bacteria, whole and fermented milk ferments and supernatants from macrophages conditioned with fermented milk do not down-regulate inflammatory activity in cultured endothelial cells, however moderate anti-inflammatory nitric oxide (NO) production by cultured endothelial cells was seen with peptides derived from the milk β -casein sequence, warranting further study."]},{"key":"dc:title","label":"Title","values":["Examining effects of milk ferment components on endothelial cell signaling in pro-inflammatory pathways"]}]}],"canonical_facts":{"dc:contributor.advisor":["Jones-Taggart, Holly"],"dc:creator":["Dobby, Kyle R.E."],"dc:date.accessioned":["2017-03-20T13:35:08Z","2022-03-29T17:39:20Z"],"dc:date.available":["2017-03-20T13:35:08Z","2022-03-29T17:39:20Z"],"dc:date.issued":["2015-08-01"],"dc:description.abstract":["Atherosclerosis is a complex, multifactorial, inflammatory disease involving cholesterol, endothelial cells, smooth muscle cells, macrophages and platelets. Atherosclerosis, and the associated cardiovascular diseases it contributes to, has been a growing health care and economic concern over the last 50 years. Bioactive peptides found in fermented milk have been shown to have cardio and athero-protective effects, but their mechanism has not been fully elucidated. The objective of this study was to test individual components of milk fermentation and macrophage conditioning in order to determine how fermented milk bioactive peptides best exert their effects. This study shows that protein secretions from bacteria, whole and fermented milk ferments and supernatants from macrophages conditioned with fermented milk do not down-regulate inflammatory activity in cultured endothelial cells, however moderate anti-inflammatory nitric oxide (NO) production by cultured endothelial cells was seen with peptides derived from the milk β -casein sequence, warranting further study."],"dc:identifier.uri":["https://hdl.handle.net/10155/728"],"dc:language.iso":["en"],"dc:subject":["Atherosclerosis","Cardiovascular disease","Bioactive peptides","Fermented milk","Endothelial cells"],"dc:title":["Examining effects of milk ferment components on endothelial cell signaling in pro-inflammatory pathways"],"dc:type":["Thesis"],"thesis:degree_discipline":["Applied Bioscience"],"thesis:degree_name":["Master of Science (MSc)"],"thesis:institution_name":["University of Ontario Institute of Technology"]},"updated_at":"2026-07-24T05:35:39Z"}