Immunomodulatory effects of lactic acid bacteria on human intestinal epithelial cells and macrophages in the context of a pro-inflammatory challenge

Immunomodulatory effects of lactic acid bacteria vary with strain and may vary with growth phase and medium. The ability of different lactobacilli strains (Lactobacillus helveticus R0052, L. rhamnosus R0011, L. rhamnosus GG) at different growth phases to modulate macrophage and intestinal epithelial cell cytokine production following a pro-inflammatory challenge was examined. Modulation of cytokine production by human macrophage cell lines (U-937) and intestinal epithelial cells (HT-29) induced by Tumor Necrosis Factor α was assayed by ELISA for interleukin-8 (IL-8). Granulocyte-macrophage colony stimulating factor (GM-CSF) production was assayed by ELISA in the HT-29 cell line. Strain-dependent differences were observed in the ability of viable bacteria and spent de Mann-Rogosa- Sharpe (MRS) broths from log versus stationary growth phase in HT-29 and U-937 cells. Overall, variation in the immunomodulatory activity of these lactic acid bacteria and spent broths reflects not only strain variation but potentially also differences in growth phase and substrate.

Name thesis:degree_name

Master of Science (MSc)

Discipline thesis:degree_discipline

Applied Bioscience

Grantor

University of Ontario Institute of Technology

Year dc:date.issued

2009

Author dc:creator

• Cooper, William

Advisors dc:contributor.advisor

• Forrester, Sean
• Strap, Janice

• Lactic acid bacteria
• Cytokine production
• Intestinal epithelial cells
• Immunomodulatory activity

Language dc:language.iso

en

Handle dc:identifier.uri

https://hdl.handle.net/10155/69

OAI identifier oai:identifier

oai:ontariotechu.scholaris.ca:10155/69