{"id":{"repo_id":"uoit","oai_identifier":"oai:ontariotechu.scholaris.ca:10155/1961"},"canonical_url":"https://search.dev.ndltd.org/etd/uoit/oai:ontariotechu.scholaris.ca:10155/1961","repository":{"repo_id":"uoit","name":"Ontario Institute of Technology","base_url":"https://ontariotechu.scholaris.ca/server/oai/request"},"display":{"title":"Synthesis and applications of oligonucleotide-bound alginate hydrogels","abstract":"This research investigates the use of circular dichroism (CD) spectroscopy for label-free detection of DNA and RNA hybridization in oligonucleotide-bound alginate hydrogels, with applications in biosensing. Incorporating 2’-fluoro modifications significantly enhanced sensitivity, with 2’-F probes outperforming unmodified DNA and RNA in both detection signal and binding specificity. In mixtures with up to four non-complementary sequences, 2’-F probes also yielded higher classification accuracy using random forest analysis, when integrating CD and thermal melt data. Concurrently, the potential of covalently bound alginate hydrogels for small interfering RNA (siRNA)-mediated gene silencing was evaluated. Covalent attachment resulted in a tenfold decrease in IC50 values compared to unbound siRNA, indicating improved gene silencing efficiency. Additionally, nuclease stability studies revealed that alginate-siRNA conjugates retained double the amount of nondegraded siRNA after 15 minutes in fetal bovine serum compared to unbound siRNA. Together, these findings highlight the versatility of alginate-oligonucleotide platforms in biosensing and therapeutic delivery applications.","abstract_html":"This research investigates the use of circular dichroism (CD) spectroscopy for label-free detection of DNA and RNA hybridization in oligonucleotide-bound alginate hydrogels, with applications in biosensing. Incorporating 2’-fluoro modifications significantly enhanced sensitivity, with 2’-F probes outperforming unmodified DNA and RNA in both detection signal and binding specificity. In mixtures with up to four non-complementary sequences, 2’-F probes also yielded higher classification accuracy using random forest analysis, when integrating CD and thermal melt data. Concurrently, the potential of covalently bound alginate hydrogels for small interfering RNA (siRNA)-mediated gene silencing was evaluated. Covalent attachment resulted in a tenfold decrease in IC50 values compared to unbound siRNA, indicating improved gene silencing efficiency. Additionally, nuclease stability studies revealed that alginate-siRNA conjugates retained double the amount of nondegraded siRNA after 15 minutes in fetal bovine serum compared to unbound siRNA. Together, these findings highlight the versatility of alginate-oligonucleotide platforms in biosensing and therapeutic delivery applications.","abstract_has_math":false,"creators":["Lubrin, Daisee K."],"institution":"University of Ontario Institute of Technology","degree_name":"Master of Science (MSc)","degree_level":null,"degree_discipline":"Applied Bioscience","degree_department":null,"school":null,"contributors":[],"advisors":["Stotesbury, Theresa"],"committee_chairs":[],"committee_members":[],"year":2025,"date_issued":"2025-06-01","date_published":"2025-06-01","updated_at":"2026-07-24T05:35:16Z","subjects":[],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/10155/1961","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Stotesbury, Theresa"]},{"key":"dc:creator","label":"Author","values":["Lubrin, Daisee K."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2025-07-21T14:26:49Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2025-07-21T14:26:49Z"]},{"key":"dc:date.issued","label":"Date","values":["2025-06-01"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Applied Bioscience"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science (MSc)"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Ontario Institute of Technology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/10155/1961"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["This research investigates the use of circular dichroism (CD) spectroscopy for label-free detection of DNA and RNA hybridization in oligonucleotide-bound alginate hydrogels, with applications in biosensing. Incorporating 2’-fluoro modifications significantly enhanced sensitivity, with 2’-F probes outperforming unmodified DNA and RNA in both detection signal and binding specificity. In mixtures with up to four non-complementary sequences, 2’-F probes also yielded higher classification accuracy using random forest analysis, when integrating CD and thermal melt data. Concurrently, the potential of covalently bound alginate hydrogels for small interfering RNA (siRNA)-mediated gene silencing was evaluated. Covalent attachment resulted in a tenfold decrease in IC50 values compared to unbound siRNA, indicating improved gene silencing efficiency. Additionally, nuclease stability studies revealed that alginate-siRNA conjugates retained double the amount of nondegraded siRNA after 15 minutes in fetal bovine serum compared to unbound siRNA. Together, these findings highlight the versatility of alginate-oligonucleotide platforms in biosensing and therapeutic delivery applications."]},{"key":"dc:title","label":"Title","values":["Synthesis and applications of oligonucleotide-bound alginate hydrogels"]}]}],"canonical_facts":{"dc:contributor.advisor":["Stotesbury, Theresa"],"dc:creator":["Lubrin, Daisee K."],"dc:date.accessioned":["2025-07-21T14:26:49Z"],"dc:date.available":["2025-07-21T14:26:49Z"],"dc:date.issued":["2025-06-01"],"dc:description.abstract":["This research investigates the use of circular dichroism (CD) spectroscopy for label-free detection of DNA and RNA hybridization in oligonucleotide-bound alginate hydrogels, with applications in biosensing. Incorporating 2’-fluoro modifications significantly enhanced sensitivity, with 2’-F probes outperforming unmodified DNA and RNA in both detection signal and binding specificity. In mixtures with up to four non-complementary sequences, 2’-F probes also yielded higher classification accuracy using random forest analysis, when integrating CD and thermal melt data. Concurrently, the potential of covalently bound alginate hydrogels for small interfering RNA (siRNA)-mediated gene silencing was evaluated. Covalent attachment resulted in a tenfold decrease in IC50 values compared to unbound siRNA, indicating improved gene silencing efficiency. Additionally, nuclease stability studies revealed that alginate-siRNA conjugates retained double the amount of nondegraded siRNA after 15 minutes in fetal bovine serum compared to unbound siRNA. Together, these findings highlight the versatility of alginate-oligonucleotide platforms in biosensing and therapeutic delivery applications."],"dc:identifier.uri":["https://hdl.handle.net/10155/1961"],"dc:language.iso":["en"],"dc:title":["Synthesis and applications of oligonucleotide-bound alginate hydrogels"],"dc:type":["Thesis"],"thesis:degree_discipline":["Applied Bioscience"],"thesis:degree_name":["Master of Science (MSc)"],"thesis:institution_name":["University of Ontario Institute of Technology"]},"updated_at":"2026-07-24T05:35:16Z"}