{"id":{"repo_id":"uoit","oai_identifier":"oai:ontariotechu.scholaris.ca:10155/1820"},"canonical_url":"https://search.dev.ndltd.org/etd/uoit/oai:ontariotechu.scholaris.ca:10155/1820","repository":{"repo_id":"uoit","name":"Ontario Institute of Technology","base_url":"https://ontariotechu.scholaris.ca/server/oai/request"},"display":{"title":"Using non-targeted mass spectrometry to identify proteomic, metabolomic and lipidomic profiles in saliva to compare the impact of prolonged vs. interrupted sitting","abstract":"Traditional sedentary physiology research focuses on targeted approaches. To explore from a new perspective, a non-targeted approach with the use of Liquid Chromatography-Mass Spectrometry (LC-MS) to assess the responsiveness of proteins and metabolites in saliva to sedentarism was employed. The study involved 24 participants that engaged in both a prolonged sitting session and an interrupted sitting session, in a randomized order. Saliva samples were collected before and after each session, including a separate baseline. Samples were purified and concentrated then analyzed on the LC-MS. A total of 2493 proteins, 17 lipids, and 11 biogenic amines were detected. 2112 proteins were significantly differentially abundant among the different session sample groups. In conclusion, prolonged sedentary behaviour of 4 hours led to a range of molecular responses that were observed through changes in the relative abundances of proteins and lipids. The results were complex and suggestive of several indicators of health deterioration.","abstract_html":"Traditional sedentary physiology research focuses on targeted approaches. To explore from a new perspective, a non-targeted approach with the use of Liquid Chromatography-Mass Spectrometry (LC-MS) to assess the responsiveness of proteins and metabolites in saliva to sedentarism was employed. The study involved 24 participants that engaged in both a prolonged sitting session and an interrupted sitting session, in a randomized order. Saliva samples were collected before and after each session, including a separate baseline. Samples were purified and concentrated then analyzed on the LC-MS. A total of 2493 proteins, 17 lipids, and 11 biogenic amines were detected. 2112 proteins were significantly differentially abundant among the different session sample groups. In conclusion, prolonged sedentary behaviour of 4 hours led to a range of molecular responses that were observed through changes in the relative abundances of proteins and lipids. The results were complex and suggestive of several indicators of health deterioration.","abstract_has_math":false,"creators":["Chauhan, Mohammed Faiz"],"institution":"University of Ontario Institute of Technology","degree_name":"Master of Science (MSc)","degree_level":null,"degree_discipline":"Applied Bioscience","degree_department":null,"school":null,"contributors":[],"advisors":["Simmons, Denina"],"committee_chairs":[],"committee_members":[],"year":2024,"date_issued":"2024-08-01","date_published":"2024-08-01","updated_at":"2026-07-24T05:35:22Z","subjects":[],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/10155/1820","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Simmons, Denina"]},{"key":"dc:creator","label":"Author","values":["Chauhan, Mohammed Faiz"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2024-08-27T15:29:24Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2024-08-27T15:29:24Z"]},{"key":"dc:date.issued","label":"Date","values":["2024-08-01"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Applied Bioscience"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science (MSc)"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Ontario Institute of Technology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/10155/1820"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Traditional sedentary physiology research focuses on targeted approaches. To explore from a new perspective, a non-targeted approach with the use of Liquid Chromatography-Mass Spectrometry (LC-MS) to assess the responsiveness of proteins and metabolites in saliva to sedentarism was employed. The study involved 24 participants that engaged in both a prolonged sitting session and an interrupted sitting session, in a randomized order. Saliva samples were collected before and after each session, including a separate baseline. Samples were purified and concentrated then analyzed on the LC-MS. A total of 2493 proteins, 17 lipids, and 11 biogenic amines were detected. 2112 proteins were significantly differentially abundant among the different session sample groups. In conclusion, prolonged sedentary behaviour of 4 hours led to a range of molecular responses that were observed through changes in the relative abundances of proteins and lipids. The results were complex and suggestive of several indicators of health deterioration."]},{"key":"dc:title","label":"Title","values":["Using non-targeted mass spectrometry to identify proteomic, metabolomic and lipidomic profiles in saliva to compare the impact of prolonged vs. interrupted sitting"]}]}],"canonical_facts":{"dc:contributor.advisor":["Simmons, Denina"],"dc:creator":["Chauhan, Mohammed Faiz"],"dc:date.accessioned":["2024-08-27T15:29:24Z"],"dc:date.available":["2024-08-27T15:29:24Z"],"dc:date.issued":["2024-08-01"],"dc:description.abstract":["Traditional sedentary physiology research focuses on targeted approaches. To explore from a new perspective, a non-targeted approach with the use of Liquid Chromatography-Mass Spectrometry (LC-MS) to assess the responsiveness of proteins and metabolites in saliva to sedentarism was employed. The study involved 24 participants that engaged in both a prolonged sitting session and an interrupted sitting session, in a randomized order. Saliva samples were collected before and after each session, including a separate baseline. Samples were purified and concentrated then analyzed on the LC-MS. A total of 2493 proteins, 17 lipids, and 11 biogenic amines were detected. 2112 proteins were significantly differentially abundant among the different session sample groups. In conclusion, prolonged sedentary behaviour of 4 hours led to a range of molecular responses that were observed through changes in the relative abundances of proteins and lipids. The results were complex and suggestive of several indicators of health deterioration."],"dc:identifier.uri":["https://hdl.handle.net/10155/1820"],"dc:language.iso":["en"],"dc:title":["Using non-targeted mass spectrometry to identify proteomic, metabolomic and lipidomic profiles in saliva to compare the impact of prolonged vs. interrupted sitting"],"dc:type":["Thesis"],"thesis:degree_discipline":["Applied Bioscience"],"thesis:degree_name":["Master of Science (MSc)"],"thesis:institution_name":["University of Ontario Institute of Technology"]},"updated_at":"2026-07-24T05:35:22Z"}