{"id":{"repo_id":"uoit","oai_identifier":"oai:ontariotechu.scholaris.ca:10155/1727"},"canonical_url":"https://search.dev.ndltd.org/etd/uoit/oai:ontariotechu.scholaris.ca:10155/1727","repository":{"repo_id":"uoit","name":"Ontario Institute of Technology","base_url":"https://ontariotechu.scholaris.ca/server/oai/request"},"display":{"title":"Silence of the fish: injection of photoswitchable short interfering RNA oligonucleotides into Japanese medaka embryos (oryzias latipes) to photochemically control gene silencing","abstract":"The siRNA duplex functions by binding to and cleaving mRNA, a process known as the RNAi pathway. Existing siRNAs face challenges such as off-target effects and unpredictable prolonged gene-silencing. To address these issues, enhancing their therapeutic potential, siRNAs can be modified controlling their role in the RNAi pathway. An Ortho-functionalized, tetrafluorinatedazobenzene was integrated into the siRNA backbone via phophoramidite chemistry to generate a class of photoswitchable F-siRNAs. These F-siRNAs retained the ability to photoisomerize from an active trans to an inactive cis state through blue and green light respectively. This thesis reported on a novel technique to inject single cell medaka embryos with both Wt and F-siRNAs. Medaka are a small freshwater teleost fish that have a number of desirable features for use as a vertebrate model in gene silencing projects. These attributes include daily spawning, a number of useful genetic strains, a completely sequenced genome, and a transparent egg.","abstract_html":"The siRNA duplex functions by binding to and cleaving mRNA, a process known as the RNAi pathway. Existing siRNAs face challenges such as off-target effects and unpredictable prolonged gene-silencing. To address these issues, enhancing their therapeutic potential, siRNAs can be modified controlling their role in the RNAi pathway. An Ortho-functionalized, tetrafluorinatedazobenzene was integrated into the siRNA backbone via phophoramidite chemistry to generate a class of photoswitchable F-siRNAs. These F-siRNAs retained the ability to photoisomerize from an active trans to an inactive cis state through blue and green light respectively. This thesis reported on a novel technique to inject single cell medaka embryos with both Wt and F-siRNAs. Medaka are a small freshwater teleost fish that have a number of desirable features for use as a vertebrate model in gene silencing projects. These attributes include daily spawning, a number of useful genetic strains, a completely sequenced genome, and a transparent egg.","abstract_has_math":false,"creators":["Mateus, Makenzie"],"institution":"University of Ontario Institute of Technology","degree_name":"Master of Science (MSc)","degree_level":null,"degree_discipline":"Applied Bioscience","degree_department":null,"school":null,"contributors":[],"advisors":["Desaulniers, Jean-Paul","Simmons, Denina"],"committee_chairs":[],"committee_members":[],"year":2023,"date_issued":"2023-12-01","date_published":"2023-12-01","updated_at":"2026-07-24T05:35:41Z","subjects":["siRNA","Antisense","Japanese medaka","Nano injection","Transgenic"],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/10155/1727","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Desaulniers, Jean-Paul","Simmons, Denina"]},{"key":"dc:creator","label":"Author","values":["Mateus, Makenzie"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2024-01-23T20:44:04Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2024-01-23T20:44:04Z"]},{"key":"dc:date.issued","label":"Date","values":["2023-12-01"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Applied Bioscience"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science (MSc)"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Ontario Institute of Technology"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["siRNA","Antisense","Japanese medaka","Nano injection","Transgenic"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/10155/1727"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The siRNA duplex functions by binding to and cleaving mRNA, a process known as the RNAi pathway. Existing siRNAs face challenges such as off-target effects and unpredictable prolonged gene-silencing. To address these issues, enhancing their therapeutic potential, siRNAs can be modified controlling their role in the RNAi pathway. An Ortho-functionalized, tetrafluorinatedazobenzene was integrated into the siRNA backbone via phophoramidite chemistry to generate a class of photoswitchable F-siRNAs. These F-siRNAs retained the ability to photoisomerize from an active trans to an inactive cis state through blue and green light respectively. This thesis reported on a novel technique to inject single cell medaka embryos with both Wt and F-siRNAs. Medaka are a small freshwater teleost fish that have a number of desirable features for use as a vertebrate model in gene silencing projects. These attributes include daily spawning, a number of useful genetic strains, a completely sequenced genome, and a transparent egg."]},{"key":"dc:title","label":"Title","values":["Silence of the fish: injection of photoswitchable short interfering RNA oligonucleotides into Japanese medaka embryos (oryzias latipes) to photochemically control gene silencing"]}]}],"canonical_facts":{"dc:contributor.advisor":["Desaulniers, Jean-Paul","Simmons, Denina"],"dc:creator":["Mateus, Makenzie"],"dc:date.accessioned":["2024-01-23T20:44:04Z"],"dc:date.available":["2024-01-23T20:44:04Z"],"dc:date.issued":["2023-12-01"],"dc:description.abstract":["The siRNA duplex functions by binding to and cleaving mRNA, a process known as the RNAi pathway. Existing siRNAs face challenges such as off-target effects and unpredictable prolonged gene-silencing. To address these issues, enhancing their therapeutic potential, siRNAs can be modified controlling their role in the RNAi pathway. An Ortho-functionalized, tetrafluorinatedazobenzene was integrated into the siRNA backbone via phophoramidite chemistry to generate a class of photoswitchable F-siRNAs. These F-siRNAs retained the ability to photoisomerize from an active trans to an inactive cis state through blue and green light respectively. This thesis reported on a novel technique to inject single cell medaka embryos with both Wt and F-siRNAs. Medaka are a small freshwater teleost fish that have a number of desirable features for use as a vertebrate model in gene silencing projects. 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