Characterizing blood plasma proteins from organ tissue in rainbow trout (Oncorhynchus mykiss) using a non-targeted proteomics approach

Protein expression patterns adapt to various cues to meet the needs of an organism. The dynamicity of an organism’s proteome can therefore reveal information about an organism’s health. Proteome databases contain limited information regarding organisms outside of medicinal biology. The Uniprot human and mouse proteomes are extensively reviewed and ~50% of both proteomes include tissue specificity, while >99% of the rainbow trout proteome lacks tissue specificity. This study aimed to expand knowledge on the rainbow trout proteome with a focus on blood plasma proteins. Blood, brain, heart, liver, kidney, and gills were collected from adult rainbow trout, plasma and tissue proteins were analyzed using liquid chromatography tandem mass spectrometry. Over 10,000 proteins were identified across all groups. Majority of the plasma proteome is shared with multiple tissue types, though 4-7% of the plasma proteome is uniquely shared with each tissue (gill > heart > liver > kidney > brain).

Name thesis:degree_name

Master of Science (MSc)

Discipline thesis:degree_discipline

Applied Bioscience

Grantor

University of Ontario Institute of Technology

Year dc:date.issued

2021

Author dc:creator

• Tannouri, Nancy

Advisor dc:contributor.advisor

• Simmons, Denina

• Proteomics
• Blood plasma
• Rainbow trout
• Fish

Language dc:language.iso

en

Handle dc:identifier.uri

https://hdl.handle.net/10155/1398

OAI identifier oai:identifier

oai:ontariotechu.scholaris.ca:10155/1398