Optimization of Cell Culture Procedures for Growing Neural Networks on Microelectrode Arrays

This thesis describes the development of an optimized method for culturing dissociated, monolayer neuronal networks from murine frontal cortex and midbrain. It is presented as a guidebook for use by cell culture specialists and laboratory personnel who require updated and complete procedures for use with microelectrode array (MEA) recording technology. Specific cell culture protocols, contamination prevention and control, as well common problems encountered within the cell culture facility, are discussed. This volume offers value and utility to the rapidly expanding fields of MEA recording and neuronal cell culture. Due to increasing interest in determining the mechanisms underlying Parkinson's disease, the newly developed procedures for mesencephalon isolation and culture on MEAs are an important research contribution.

Grantor dc:publisher

University of North Texas

Year dc:date

2007

Author dc:creator

• Santa Maria, Cara L.

Contributors dc:contributor

• Gross, Guenter W.
• Beitinger, Thomas L.
• Fuchs, Jannon L.

• cell culture
• microelectrode array
• protocols
• midbrain
• frontal cortex
• neuronal networks
• Cell culture.
• Neural networks (Neurobiology)
• Microelectrodes.
• Mice -- Nervous system.

Statement dc:rights

• Public
• Copyright
• Santa Maria, Cara L.
• Copyright is held by the author, unless otherwise noted. All rights reserved.

Language dc:language

English

Identifier

oclc: 227206451
https://digital.library.unt.edu/ark:/67531/metadc5126/
ark: ark:/67531/metadc5126

OAI identifier oai:identifier

info:ark/67531/metadc5126