Hematocrit, hematocrit Regulation and its effect on oxygen consumption in the late stage chicken embryo (Gallus domesticus).

Hematocrit and hematocrit regulation have the potential to affect developing embryos. To examine the ability of chicken embryos at day 15 to regulate hematocrit, they were subjected to either repeated saline injections (5% of total blood volume) or repeated blood removal (5% of total blood volume). Embryos showed an ability to maintain hematocrit (~20%) despite blood volume increases up to 115% of initial blood volume. Embryos were not able to maintain hematocrit in the face of dramatic blood volume loss. Oxygen consumption of embryos could be affected by their level of hematocrit. To examine this, chicken embryos at day 15, 16, and 17 of incubation were given a high hematocrit (~50-60%) sample of blood (400 μl) to artificially increase the hematocrit of the embryos (~10-12%). Despite the increase in oxygen availability, when monitored over a period of six hours, embryos showed no difference (0.36 ± 0.01 (ml O2 - min-1- egg-1) in metabolism from baseline measurements at day 15, 16 and 17.

Grantor dc:publisher

University of North Texas

Year dc:date

2004

Author dc:creator

• Khorrami, Sheva

Contributors dc:contributor

• Burggren, Warren W.
• Bishop, Kathleen L.
• Dzialowski, Edward M.

• Hematocrit.
• Chickens -- Embryos.
• Chickens -- Respiration.
• oxygen consumption
• chicken embryos
• blood removal
• hematocrit regulation
• erythrocythemia

Statement dc:rights

• Public
• Copyright
• Khorrami, Sheva
• Copyright is held by the author, unless otherwise noted. All rights reserved.

Language dc:language

English

Identifier

oclc: 56636079
https://digital.library.unt.edu/ark:/67531/metadc4598/
ark: ark:/67531/metadc4598

OAI identifier oai:identifier

info:ark/67531/metadc4598