Identification and quantification of lipid metabolites in cotton fibers: Reconciliation with metabolic pathway predictions from DNA databases.

The lipid composition of cotton (Gossypium hirsutum, L) fibers was determined. Fatty acid profiles revealed that linolenate and palmitate were the most abundant fatty acids present in fiber cells. Phosphatidylcholine was the predominant lipid class in fiber cells, while phosphatidylethanolamine, phosphatidylinositol and digalactosyldiacylglycerol were also prevalent. An unusually high amount of phosphatidic acid was observed in frozen cotton fibers. Phospholipase D activity assays revealed that this enzyme readily hydrolyzed radioactive phosphatidylcholine into phosphatidic acid. A profile of expressed sequence tags (ESTs) for genes involved in lipid metabolism in cotton fibers was also obtained. This EST profile along with our lipid metabolite data was used to predict lipid metabolic pathways in cotton fiber cells.

Grantor dc:publisher

University of North Texas

Year dc:date

2004

Author dc:creator

• Wanjie, Sylvia W.

Contributors dc:contributor

• Chapman, Kent D.
• Pirtle, Robert M.
• Dickstein, Rebecca

• Cotton -- Genetics.
• Lipids -- Metabolism.
• fatty acids
• lipid metabolism
• cotton fibers
• plant membranes
• DNA databases

Statement dc:rights

• Public
• Copyright
• Wanjie, Sylvia W.
• Copyright is held by the author, unless otherwise noted. All rights reserved.

Language dc:language

English

Identifier

oclc: 55804232
https://digital.library.unt.edu/ark:/67531/metadc4474/
ark: ark:/67531/metadc4474

OAI identifier oai:identifier

info:ark/67531/metadc4474