{"id":{"repo_id":"unt","oai_identifier":"info:ark/67531/metadc3341"},"canonical_url":"https://search.dev.ndltd.org/etd/unt/info:ark/67531/metadc3341","repository":{"repo_id":"unt","name":"University of North Texas","base_url":"https://digital.library.unt.edu/oai/"},"display":{"title":"Tobacco Phospholipase D β1: Molecular Cloning and Biochemical Characterization","abstract":"Transgenic tobacco plants were developed containing a partial PLD clone in antisense orientation. The PLD isoform targeted by the insertion was identified. A PLD clone was isolated from a cDNA library using the partial PLD as a probe: Nt10B1 shares 92% identity with PLDβ1 from tomato but lacks the C2 domain. PCR analysis confirmed insertion of the antisense fragment into the plants: three introns distinguished the endogenous gene from the transgene. PLD activity was assayed in leaf homogenates in PLDβ/g conditions. When phosphatidylcholine was utilized as a substrate, no significant difference in transphosphatidylation activity was observed. However, there was a reduction in NAPE hydrolysis in extracts of two transgenic plants. In one of these, a reduction in elicitor- induced PAL expression was also observed.","abstract_html":"Transgenic tobacco plants were developed containing a partial PLD clone in antisense orientation. The PLD isoform targeted by the insertion was identified. A PLD clone was isolated from a cDNA library using the partial PLD as a probe: Nt10B1 shares 92% identity with PLDβ1 from tomato but lacks the C2 domain. PCR analysis confirmed insertion of the antisense fragment into the plants: three introns distinguished the endogenous gene from the transgene. PLD activity was assayed in leaf homogenates in PLDβ/g conditions. When phosphatidylcholine was utilized as a substrate, no significant difference in transphosphatidylation activity was observed. However, there was a reduction in NAPE hydrolysis in extracts of two transgenic plants. In one of these, a reduction in elicitor- induced PAL expression was also observed.","abstract_has_math":false,"creators":["Hodson, Jane E."],"institution":"University of North Texas","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Chapman, Kent D.","Pirtle, Robert M.","Knesek, John"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2002,"date_issued":"2002-12","date_published":"2002-12","updated_at":"2026-07-24T05:34:52Z","subjects":["Tobacco.","Phospholipases.","Molecular cloning.","PLDβ1","Phospholipase D β1","phospholipid metabolism","tobacco","lipid signaling"],"languages":["English"],"rights":["Public","Copyright","Hodson, Jane E.","Copyright is held by the author, unless otherwise noted. 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When phosphatidylcholine was utilized as a substrate, no significant difference in transphosphatidylation activity was observed. However, there was a reduction in NAPE hydrolysis in extracts of two transgenic plants. In one of these, a reduction in elicitor- induced PAL expression was also observed."]},{"key":"dc:format","label":"Dc Format","values":["Text"]},{"key":"dc:title","label":"Title","values":["Tobacco Phospholipase D β1: Molecular Cloning and Biochemical Characterization"]}]}],"canonical_facts":{"dc:contributor":["Chapman, Kent D.","Pirtle, Robert M.","Knesek, John"],"dc:creator":["Hodson, Jane E."],"dc:date":["2002-12"],"dc:description":["Transgenic tobacco plants were developed containing a partial PLD clone in antisense orientation. The PLD isoform targeted by the insertion was identified. A PLD clone was isolated from a cDNA library using the partial PLD as a probe: Nt10B1 shares 92% identity with PLDβ1 from tomato but lacks the C2 domain. PCR analysis confirmed insertion of the antisense fragment into the plants: three introns distinguished the endogenous gene from the transgene. PLD activity was assayed in leaf homogenates in PLDβ/g conditions. When phosphatidylcholine was utilized as a substrate, no significant difference in transphosphatidylation activity was observed. However, there was a reduction in NAPE hydrolysis in extracts of two transgenic plants. In one of these, a reduction in elicitor- induced PAL expression was also observed."],"dc:format":["Text"],"dc:identifier":["oclc: 52162126","doi: 10.12794/metadc3341","https://digital.library.unt.edu/ark:/67531/metadc3341/","ark: ark:/67531/metadc3341"],"dc:language":["English"],"dc:publisher":["University of North Texas"],"dc:rights":["Public","Copyright","Hodson, Jane E.","Copyright is held by the author, unless otherwise noted. 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