Characterization of the Aspartate Transcarbamoylase that is Found in the pyrBC" Complex of Bordetella Pertussis

An aspartate transcarbamoylase (ATCase) gene from Bordetella pertussis was amplified by PCR and ligated into pT-ADV for expression in Escherichia coli. This particular ATCase (pyrB) was an inactive gene found adjacent to an inactive dihydroorotase (DHOase) gene (pyrC'). This experiment was undertaken to determine whether this pyrB gene was capable of expression alone or if it was capable of expression only when cotransformed with a functional pyrC'. When transformed into E. coli TB2 pyrB-, the gene did not produce any ATCase activity. The gene was then co-transformed into E. coli TB2 pyrB- along with a plasmid containing the pyrC' gene from Pseudomonas aeruginosa and assayed for ATCase activity. Negative results were again recorded.

Grantor dc:publisher

University of North Texas

Year dc:date

2001

Author dc:creator

• Dill, Michael T

Contributors dc:contributor

• Farinha, Mark A.
• O'Donovan, Gerard A.
• Benjamin, Robert C.

• Pyrimidine nucleotides -- Metabolism.
• Bordetella pertussis.
• aspartate transcarbamoylase
• Bordetella pertussis
• ATCase
• pyrBC complex
• gene expression

Statement dc:rights

• Public
• Copyright
• Dill, Michael T
• Copyright is held by the author, unless otherwise noted. All rights reserved.

Language dc:language

English

Identifier

oclc: 51977993
https://digital.library.unt.edu/ark:/67531/metadc3057/
ark: ark:/67531/metadc3057

OAI identifier oai:identifier

info:ark/67531/metadc3057