Potential for selection of new peanut genotypes with enhanced polyphenol antioxidant content
type: CreativeWork
description: The goal of this project was to determine the potential for the breeding of new peanut genotypes with enhanced polyphenol content and antioxidant capacity. The specific objectives of this project were: 1) to estimate the genotype (G), environment (E) and G x E influence on the expression of polyphenols in a Recombinant Inbred Line (RIL) peanut population; 2) to study the molecular basis of polyphenol biosynthesis in peanuts using the shotgun proteomics approach; 3) to screen for new phenolic compounds in a water-soluble peanut extract using LC-MS/MS and propose phenolic biosynthesis pathways, and 4) to investigate the feasibility of developing a simple nanoparticle-based test for the rapid and low-cost phenotyping of antioxidant capacity for rapid selection of antioxidant capacity in breeding and segregating populations. The genotype (G), environment (E), and G x E influence on antioxidant expression investigated in the RIL population showed significant genotypic and environmental effects, but non-significant G x E effects suggesting strong genetic control and moderate heritability. Quantifications of known polyphenols in selected RILs showed increased ferulic acid, p-coumaric acid, salicylic acid, resveratrol, and daidzein with the enzymatic extraction, indicating the importance of matrix-bound polyphenolic compounds and corresponded to respective ORAC assay values. The shotgun proteomics revealed differential protein abundance in the high antioxidant expressing RIL in carbohydrate and protein metabolism, stilbene and flavonoid biosynthesis, anabolic and catabolic pathways. These metabolic changes contributed towards phenylalanine biosynthesis lead to the central phenylpropanoid pathway and subsequent synthesis of identified polyphenols. Enzymes showing significant differential abundance were mapped to describe metabolic changes and biosynthesis pathways related to high antioxidant expression which have potential use as biomarkers for related compounds. In particul…
abstract: The goal of this project was to determine the potential for the breeding of new peanut genotypes with enhanced polyphenol content and antioxidant capacity. The specific objectives of this project were: 1) to estimate the genotype (G), environment (E) and G x E influence on the expression of polyphenols in a Recombinant Inbred Line (RIL) peanut population; 2) to study the molecular basis of polyphenol biosynthesis in peanuts using the shotgun proteomics approach; 3) to screen for new phenolic compounds in a water-soluble peanut extract using LC-MS/MS and propose phenolic biosynthesis pathways, and 4) to investigate the feasibility of developing a simple nanoparticle-based test for the rapid and low-cost phenotyping of antioxidant capacity for rapid selection of antioxidant capacity in breeding and segregating populations. The genotype (G), environment (E), and G x E influence on antioxidant expression investigated in the RIL population showed significant genotypic and environmental effects, but non-significant G x E effects suggesting strong genetic control and moderate heritability. Quantifications of known polyphenols in selected RILs showed increased ferulic acid, p-coumaric acid, salicylic acid, resveratrol, and daidzein with the enzymatic extraction, indicating the importance of matrix-bound polyphenolic compounds and corresponded to respective ORAC assay values. The shotgun proteomics revealed differential protein abundance in the high antioxidant expressing RIL in carbohydrate and protein metabolism, stilbene and flavonoid biosynthesis, anabolic and catabolic pathways. These metabolic changes contributed towards phenylalanine biosynthesis lead to the central phenylpropanoid pathway and subsequent synthesis of identified polyphenols. Enzymes showing significant differential abundance were mapped to describe metabolic changes and biosynthesis pathways related to high antioxidant expression which have potential use as biomarkers for related compounds. In particul…
author: Poon, Yan Yee
language: EN
keywords: Polyphenols, Peanut, Antioxidant, Silver nanoparticles, Proteomics
license: https://purl.org/coar/access_right/c_abf2
license: https://creativecommons.org/licenses/by-nc-nd/3.0/au/
date published: 2020
date modified: 2026-07-24T05:32:40Z
provider: University of New South Wales
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: UNSW, Sydney

PropertyValue
property ID: Handle
value: http://hdl.handle.net/1959.4/70069

PropertyValue
property ID: DOI
value: 10.26190/unsworks/22055