{"id":{"repo_id":"unr","oai_identifier":"oai:scholarwolf.unr.edu:11714/645"},"canonical_url":"https://search.dev.ndltd.org/etd/unr/oai:scholarwolf.unr.edu:11714/645","repository":{"repo_id":"unr","name":"University of Nevada - Reno","base_url":"https://scholarwolf.unr.edu/server/oai/request"},"display":{"title":"Stem-Cell Like PDGFR? Cells are Induced in Chronic Intestinal Partial Obstruction","abstract":"The gastrointestinal tract harbors a wide array of smooth muscle cells, motor neurons, and epithelial and stem cells. This makes it an attractive model system for the study of regeneration following mechanical injury. This project investigated the regenerative capacity of the gut by injuring the murine gastrointestinal tract and characterizing the changes that occur in several cell populations critical to gastroenterological function. To accomplish this, a chronic partial intestinal obstruction surgery was used to stimulate intestinal tissue, and changes to cellular phenotype and behavior were characterized using histology and immunofluorescence. We found that the injury induced smooth muscle hypertrophy, which involved the proliferation of CD34+/CD29-/CD45+ cells. Moreover, mature smooth muscle cells dedifferentiated into a proliferative, PDGFR?+ phenotype under hypertrophic and cultured conditions. Future directions will evaluate the potential of these cells for use in cell therapy, and will translate these findings to analagous aganglionic human disorders.","abstract_html":"The gastrointestinal tract harbors a wide array of smooth muscle cells, motor neurons, and epithelial and stem cells. This makes it an attractive model system for the study of regeneration following mechanical injury. This project investigated the regenerative capacity of the gut by injuring the murine gastrointestinal tract and characterizing the changes that occur in several cell populations critical to gastroenterological function. To accomplish this, a chronic partial intestinal obstruction surgery was used to stimulate intestinal tissue, and changes to cellular phenotype and behavior were characterized using histology and immunofluorescence. We found that the injury induced smooth muscle hypertrophy, which involved the proliferation of CD34+/CD29-/CD45+ cells. Moreover, mature smooth muscle cells dedifferentiated into a proliferative, PDGFR?+ phenotype under hypertrophic and cultured conditions. Future directions will evaluate the potential of these cells for use in cell therapy, and will translate these findings to analagous aganglionic human disorders.","abstract_has_math":false,"creators":["Fuchs, Robert M."],"institution":"University of Nevada, Reno","degree_name":"Biochem and Molecular Biology","degree_level":"Honors Thesis","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Ro, Seungil"],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015","date_published":"2015","updated_at":"2026-07-27T21:47:58Z","subjects":[],"languages":["en_US","English"],"rights":["In Copyright(All Rights Reserved)"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/11714/645","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Ro, Seungil"]},{"key":"dc:creator","label":"Author","values":["Fuchs, Robert M."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2017-01-24T23:09:19Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2017-01-24T23:09:19Z"]},{"key":"dc:date.issued","label":"Date","values":["2015"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Honors Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Biochem and Molecular Biology"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Nevada, Reno"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["English"]},{"key":"dc:language.iso","label":"Language (ISO)","values":["en_US"]},{"key":"dc:rights","label":"Dc Rights","values":["In Copyright(All Rights Reserved)"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/11714/645"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The University of Nevada, Reno Libraries will promptly respond to removal requests related to content that violates intellectual property laws, data protections, or has been uploaded without creator consent. Takedown notices should be directed to our ScholarWolf team (scholarwolf@library.unr.edu) with information about the object, including its full URL and the nature of your complaint."]},{"key":"dc:description.abstract","label":"Abstract","values":["The gastrointestinal tract harbors a wide array of smooth muscle cells, motor neurons, and epithelial and stem cells. This makes it an attractive model system for the study of regeneration following mechanical injury. This project investigated the regenerative capacity of the gut by injuring the murine gastrointestinal tract and characterizing the changes that occur in several cell populations critical to gastroenterological function. To accomplish this, a chronic partial intestinal obstruction surgery was used to stimulate intestinal tissue, and changes to cellular phenotype and behavior were characterized using histology and immunofluorescence. We found that the injury induced smooth muscle hypertrophy, which involved the proliferation of CD34+/CD29-/CD45+ cells. Moreover, mature smooth muscle cells dedifferentiated into a proliferative, PDGFR?+ phenotype under hypertrophic and cultured conditions. Future directions will evaluate the potential of these cells for use in cell therapy, and will translate these findings to analagous aganglionic human disorders."]},{"key":"dc:format","label":"Dc Format","values":["PDF"]},{"key":"dc:title","label":"Title","values":["Stem-Cell Like PDGFR? Cells are Induced in Chronic Intestinal Partial Obstruction"]}]}],"canonical_facts":{"dc:contributor.advisor":["Ro, Seungil"],"dc:creator":["Fuchs, Robert M."],"dc:date.accessioned":["2017-01-24T23:09:19Z"],"dc:date.available":["2017-01-24T23:09:19Z"],"dc:date.issued":["2015"],"dc:description":["The University of Nevada, Reno Libraries will promptly respond to removal requests related to content that violates intellectual property laws, data protections, or has been uploaded without creator consent. Takedown notices should be directed to our ScholarWolf team (scholarwolf@library.unr.edu) with information about the object, including its full URL and the nature of your complaint."],"dc:description.abstract":["The gastrointestinal tract harbors a wide array of smooth muscle cells, motor neurons, and epithelial and stem cells. This makes it an attractive model system for the study of regeneration following mechanical injury. This project investigated the regenerative capacity of the gut by injuring the murine gastrointestinal tract and characterizing the changes that occur in several cell populations critical to gastroenterological function. To accomplish this, a chronic partial intestinal obstruction surgery was used to stimulate intestinal tissue, and changes to cellular phenotype and behavior were characterized using histology and immunofluorescence. We found that the injury induced smooth muscle hypertrophy, which involved the proliferation of CD34+/CD29-/CD45+ cells. Moreover, mature smooth muscle cells dedifferentiated into a proliferative, PDGFR?+ phenotype under hypertrophic and cultured conditions. Future directions will evaluate the potential of these cells for use in cell therapy, and will translate these findings to analagous aganglionic human disorders."],"dc:format":["PDF"],"dc:identifier.uri":["http://hdl.handle.net/11714/645"],"dc:language":["English"],"dc:language.iso":["en_US"],"dc:rights":["In Copyright(All Rights Reserved)"],"dc:title":["Stem-Cell Like PDGFR? Cells are Induced in Chronic Intestinal Partial Obstruction"],"dc:type":["Thesis"],"thesis:degree_level":["Honors Thesis"],"thesis:degree_name":["Biochem and Molecular Biology"],"thesis:institution_name":["University of Nevada, Reno"]},"updated_at":"2026-07-27T21:47:58Z"}