Characterization and Heterologous Expression of Recombinant Drosophila melanogaster Fatty Acyl CoA Reductase

Insect genomes have multiple fatty acyl CoA reductases (FARs), which implies varying substrate and product profiles. However, very few of these FARs have been characterized. Expression of the FAR CG18031 from Drosophila melanogaster in Spodoptera frugiperda (Sf)9 cells will allow for functional characterization of the enzyme. Substrate and chain-length specificity can then be determined by gas chromatography analysis. Results from the functional characterization will provide a greater insight into the biochemistry of insect hydrocarbon synthesis. This information can be used to implement insect FARs into a plant system for increased hydrocarbon production and more cost-efficient biofuels.

Name thesis:degree_name

Biochem and Molecular Biology

Level thesis:degree_level

Honors Thesis

Grantor

University of Nevada, Reno

Year dc:date.issued

2012

Author dc:creator

• Nowinski, Magda

Advisor dc:contributor.advisor

• Tittiger, Claus R.

Statement dc:rights

• In Copyright(All Rights Reserved)

Language dc:language.iso

en_US, English

Handle dc:identifier.uri

http://hdl.handle.net/11714/626

OAI identifier oai:identifier

oai:scholarwolf.unr.edu:11714/626