{"id":{"repo_id":"unr","oai_identifier":"oai:scholarwolf.unr.edu:11714/4377"},"canonical_url":"https://search.dev.ndltd.org/etd/unr/oai:scholarwolf.unr.edu:11714/4377","repository":{"repo_id":"unr","name":"University of Nevada - Reno","base_url":"https://scholarwolf.unr.edu/server/oai/request"},"display":{"title":"Regulation of Neuronal Polarity by Wnt Signaling","abstract":"Wnts are highly conserved secreted glycoproteins that function in many developmental processes. Cell surface receptors, including the Frizzleds (Fz), ROR, and RYK families of proteins, bind Wnts to activate a number of downstream pathways. Wnts act redundantly to regulate the anteroposterior polarity of mechanosensory neurons ALM and PLM in C. elegans (Hilliard and Bargmann, 2006; Prasad and Clark, 2006). With the exception of one wnt/fz pair (lin-44/lin-17) the specific interactions between Wnt ligands and cell surface receptors in this process are unknown. A genetic screen was undertaken to identify genes that function in the pathway of either of two Wnts (egl-20 and cwn-1) known to determine ALM polarity. New alleles of cwn-1/wnt, lin-44/wnt and lin-17/fz were isolated as well as a mutation that affects both ALM and PLM polarity. Expression of PLR-1 in the mechanosensory neurons caused polarity defects indicating that Fz can influence their polarity. mom-5/fz RNAi generated polarity defects in both ALM and PLM supporting its involvement in this process. cfz-2/fz;lin-17 double mutants were found to enhance the ALN cell fate defects observed in lin-17 mutants. Finally the transgenic lines expressing PLR-1 in the mechanosensory neurons were crossed with cam-1 mutants enhancing the ALM defects in one line, and with lin-18 mutants attenuating the PLM polarity defects in both lines. This suggests that lin-18 and cam-1 might function in a signaling or regulatory capacity in ALM and PLM polarization.","abstract_html":"Wnts are highly conserved secreted glycoproteins that function in many developmental processes. Cell surface receptors, including the Frizzleds (Fz), ROR, and RYK families of proteins, bind Wnts to activate a number of downstream pathways. Wnts act redundantly to regulate the anteroposterior polarity of mechanosensory neurons ALM and PLM in C. elegans (Hilliard and Bargmann, 2006; Prasad and Clark, 2006). With the exception of one wnt/fz pair (lin-44/lin-17) the specific interactions between Wnt ligands and cell surface receptors in this process are unknown. A genetic screen was undertaken to identify genes that function in the pathway of either of two Wnts (egl-20 and cwn-1) known to determine ALM polarity. New alleles of cwn-1/wnt, lin-44/wnt and lin-17/fz were isolated as well as a mutation that affects both ALM and PLM polarity. Expression of PLR-1 in the mechanosensory neurons caused polarity defects indicating that Fz can influence their polarity. mom-5/fz RNAi generated polarity defects in both ALM and PLM supporting its involvement in this process. cfz-2/fz;lin-17 double mutants were found to enhance the ALN cell fate defects observed in lin-17 mutants. Finally the transgenic lines expressing PLR-1 in the mechanosensory neurons were crossed with cam-1 mutants enhancing the ALM defects in one line, and with lin-18 mutants attenuating the PLM polarity defects in both lines. This suggests that lin-18 and cam-1 might function in a signaling or regulatory capacity in ALM and PLM polarization.","abstract_has_math":false,"creators":["Jones, Kathleen Q."],"institution":null,"degree_name":null,"degree_level":"Master's Degree","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Clark, Scott G."],"committee_chairs":[],"committee_members":["Kidd, Thomas","Mastick, Grant","Mastick, Cynthia"],"year":2010,"date_issued":"2010","date_published":"2010","updated_at":"2026-07-27T21:45:34Z","subjects":["C. elegans","polarity","Wnt"],"languages":[],"rights":["In Copyright(All Rights Reserved)"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/11714/4377","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Clark, Scott G."]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Kidd, Thomas","Mastick, Grant","Mastick, Cynthia"]},{"key":"dc:creator","label":"Author","values":["Jones, Kathleen Q."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2018-09-06T17:33:53Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2018-09-06T17:33:53Z"]},{"key":"dc:date.issued","label":"Date","values":["2010"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Master's Degree"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["C. elegans","polarity","Wnt"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["In Copyright(All Rights Reserved)"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/11714/4377"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Wnts are highly conserved secreted glycoproteins that function in many developmental processes. Cell surface receptors, including the Frizzleds (Fz), ROR, and RYK families of proteins, bind Wnts to activate a number of downstream pathways. Wnts act redundantly to regulate the anteroposterior polarity of mechanosensory neurons ALM and PLM in C. elegans (Hilliard and Bargmann, 2006; Prasad and Clark, 2006). With the exception of one wnt/fz pair (lin-44/lin-17) the specific interactions between Wnt ligands and cell surface receptors in this process are unknown. A genetic screen was undertaken to identify genes that function in the pathway of either of two Wnts (egl-20 and cwn-1) known to determine ALM polarity. New alleles of cwn-1/wnt, lin-44/wnt and lin-17/fz were isolated as well as a mutation that affects both ALM and PLM polarity. Expression of PLR-1 in the mechanosensory neurons caused polarity defects indicating that Fz can influence their polarity. mom-5/fz RNAi generated polarity defects in both ALM and PLM supporting its involvement in this process. cfz-2/fz;lin-17 double mutants were found to enhance the ALN cell fate defects observed in lin-17 mutants. Finally the transgenic lines expressing PLR-1 in the mechanosensory neurons were crossed with cam-1 mutants enhancing the ALM defects in one line, and with lin-18 mutants attenuating the PLM polarity defects in both lines. This suggests that lin-18 and cam-1 might function in a signaling or regulatory capacity in ALM and PLM polarization."]},{"key":"dc:format","label":"Dc Format","values":["PDF"]},{"key":"dc:title","label":"Title","values":["Regulation of Neuronal Polarity by Wnt Signaling"]}]}],"canonical_facts":{"dc:contributor.advisor":["Clark, Scott G."],"dc:contributor.committeemember":["Kidd, Thomas","Mastick, Grant","Mastick, Cynthia"],"dc:creator":["Jones, Kathleen Q."],"dc:date.accessioned":["2018-09-06T17:33:53Z"],"dc:date.available":["2018-09-06T17:33:53Z"],"dc:date.issued":["2010"],"dc:description.abstract":["Wnts are highly conserved secreted glycoproteins that function in many developmental processes. Cell surface receptors, including the Frizzleds (Fz), ROR, and RYK families of proteins, bind Wnts to activate a number of downstream pathways. Wnts act redundantly to regulate the anteroposterior polarity of mechanosensory neurons ALM and PLM in C. elegans (Hilliard and Bargmann, 2006; Prasad and Clark, 2006). With the exception of one wnt/fz pair (lin-44/lin-17) the specific interactions between Wnt ligands and cell surface receptors in this process are unknown. A genetic screen was undertaken to identify genes that function in the pathway of either of two Wnts (egl-20 and cwn-1) known to determine ALM polarity. New alleles of cwn-1/wnt, lin-44/wnt and lin-17/fz were isolated as well as a mutation that affects both ALM and PLM polarity. Expression of PLR-1 in the mechanosensory neurons caused polarity defects indicating that Fz can influence their polarity. mom-5/fz RNAi generated polarity defects in both ALM and PLM supporting its involvement in this process. cfz-2/fz;lin-17 double mutants were found to enhance the ALN cell fate defects observed in lin-17 mutants. Finally the transgenic lines expressing PLR-1 in the mechanosensory neurons were crossed with cam-1 mutants enhancing the ALM defects in one line, and with lin-18 mutants attenuating the PLM polarity defects in both lines. This suggests that lin-18 and cam-1 might function in a signaling or regulatory capacity in ALM and PLM polarization."],"dc:format":["PDF"],"dc:identifier.uri":["http://hdl.handle.net/11714/4377"],"dc:rights":["In Copyright(All Rights Reserved)"],"dc:subject":["C. elegans","polarity","Wnt"],"dc:title":["Regulation of Neuronal Polarity by Wnt Signaling"],"dc:type":["Thesis"],"thesis:degree_level":["Master's Degree"]},"updated_at":"2026-07-27T21:45:34Z"}