{"id":{"repo_id":"unr","oai_identifier":"oai:scholarwolf.unr.edu:11714/364"},"canonical_url":"https://search.dev.ndltd.org/etd/unr/oai:scholarwolf.unr.edu:11714/364","repository":{"repo_id":"unr","name":"University of Nevada - Reno","base_url":"https://scholarwolf.unr.edu/server/oai/request"},"display":{"title":"Separation and Purification of a Monoclonal Antibody for use in Patients Suffering from Wet Macular Degeneration","abstract":"Novel process designs were created in an effort to optimize the efficiency of monoclonal antibody separation and purification for an industrial process. A base case was established that utilized common protein separation techniques such as cation exchange chromatography, anion exchange chromatography, and hydrophobic interaction chromatography (HIC). Alternate process designs were also made that utilized immobilized metal affinity chromatography (IMAC) and various multimodal chromatography mediums. Experiments were conducted to determine the most effective mediums that could be used in the process design. To better analyze the new alternate plans, an economic analysis was conducted which determined what alternate plan would be more cost effective. From analysis, alternate design 1 and alternate design 2a were determined to be the preferred design due to enhanced performance and lesser overall capital cost.","abstract_html":"Novel process designs were created in an effort to optimize the efficiency of monoclonal antibody separation and purification for an industrial process. A base case was established that utilized common protein separation techniques such as cation exchange chromatography, anion exchange chromatography, and hydrophobic interaction chromatography (HIC). Alternate process designs were also made that utilized immobilized metal affinity chromatography (IMAC) and various multimodal chromatography mediums. Experiments were conducted to determine the most effective mediums that could be used in the process design. To better analyze the new alternate plans, an economic analysis was conducted which determined what alternate plan would be more cost effective. From analysis, alternate design 1 and alternate design 2a were determined to be the preferred design due to enhanced performance and lesser overall capital cost.","abstract_has_math":false,"creators":["Abbas, Syed","Sulharia, Amaad","Clary, Wesley","Del Bianco, Marcus","Guillen, Dario","Harder, Tanaka","Henrique Israel, Luis","Lopez, Joel","McMahon, Adam M.","Schmidt, Monica","Worsnop, Stephanie","Zanette, Rikelli"],"institution":"University of Nevada, Reno","degree_name":"Chemical Engineering","degree_level":"Honors Thesis","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Fuchs, Alan W."],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015","date_published":"2015","updated_at":"2026-07-27T21:45:45Z","subjects":[],"languages":["en_US"],"rights":["In Copyright(All Rights Reserved)"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/11714/364","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Fuchs, Alan W."]},{"key":"dc:creator","label":"Author","values":["Abbas, Syed","Sulharia, Amaad","Clary, Wesley","Del Bianco, Marcus","Guillen, Dario","Harder, Tanaka","Henrique Israel, Luis","Lopez, Joel","McMahon, Adam M.","Schmidt, Monica","Worsnop, Stephanie","Zanette, Rikelli"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2017-01-24T23:08:50Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2017-01-24T23:08:50Z"]},{"key":"dc:date.issued","label":"Date","values":["2015"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Honors Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Chemical Engineering"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Nevada, Reno"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en_US"]},{"key":"dc:rights","label":"Dc Rights","values":["In Copyright(All Rights Reserved)"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/11714/364"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The University of Nevada, Reno Libraries will promptly respond to removal requests related to content that violates intellectual property laws, data protections, or has been uploaded without creator consent. Takedown notices should be directed to our ScholarWolf team (scholarwolf@library.unr.edu) with information about the object, including its full URL and the nature of your complaint."]},{"key":"dc:description.abstract","label":"Abstract","values":["Novel process designs were created in an effort to optimize the efficiency of monoclonal antibody separation and purification for an industrial process. A base case was established that utilized common protein separation techniques such as cation exchange chromatography, anion exchange chromatography, and hydrophobic interaction chromatography (HIC). Alternate process designs were also made that utilized immobilized metal affinity chromatography (IMAC) and various multimodal chromatography mediums. Experiments were conducted to determine the most effective mediums that could be used in the process design. To better analyze the new alternate plans, an economic analysis was conducted which determined what alternate plan would be more cost effective. From analysis, alternate design 1 and alternate design 2a were determined to be the preferred design due to enhanced performance and lesser overall capital cost."]},{"key":"dc:format","label":"Dc Format","values":["PDF"]},{"key":"dc:title","label":"Title","values":["Separation and Purification of a Monoclonal Antibody for use in Patients Suffering from Wet Macular Degeneration"]}]}],"canonical_facts":{"dc:contributor.advisor":["Fuchs, Alan W."],"dc:creator":["Abbas, Syed","Sulharia, Amaad","Clary, Wesley","Del Bianco, Marcus","Guillen, Dario","Harder, Tanaka","Henrique Israel, Luis","Lopez, Joel","McMahon, Adam M.","Schmidt, Monica","Worsnop, Stephanie","Zanette, Rikelli"],"dc:date.accessioned":["2017-01-24T23:08:50Z"],"dc:date.available":["2017-01-24T23:08:50Z"],"dc:date.issued":["2015"],"dc:description":["The University of Nevada, Reno Libraries will promptly respond to removal requests related to content that violates intellectual property laws, data protections, or has been uploaded without creator consent. Takedown notices should be directed to our ScholarWolf team (scholarwolf@library.unr.edu) with information about the object, including its full URL and the nature of your complaint."],"dc:description.abstract":["Novel process designs were created in an effort to optimize the efficiency of monoclonal antibody separation and purification for an industrial process. A base case was established that utilized common protein separation techniques such as cation exchange chromatography, anion exchange chromatography, and hydrophobic interaction chromatography (HIC). Alternate process designs were also made that utilized immobilized metal affinity chromatography (IMAC) and various multimodal chromatography mediums. Experiments were conducted to determine the most effective mediums that could be used in the process design. To better analyze the new alternate plans, an economic analysis was conducted which determined what alternate plan would be more cost effective. From analysis, alternate design 1 and alternate design 2a were determined to be the preferred design due to enhanced performance and lesser overall capital cost."],"dc:format":["PDF"],"dc:identifier.uri":["http://hdl.handle.net/11714/364"],"dc:language.iso":["en_US"],"dc:rights":["In Copyright(All Rights Reserved)"],"dc:title":["Separation and Purification of a Monoclonal Antibody for use in Patients Suffering from Wet Macular Degeneration"],"dc:type":["Thesis"],"thesis:degree_level":["Honors Thesis"],"thesis:degree_name":["Chemical Engineering"],"thesis:institution_name":["University of Nevada, Reno"]},"updated_at":"2026-07-27T21:45:45Z"}