Characterization of the Genes Essential for Mouse Spermatogenesis
type: CreativeWork
description: Spermatogenesis is a complex process that starts with the proliferation of differentiated spermatogonia through mitotic division. Primary spermatocytes produced from differentiated spermatogonia enter the prolonged prophase of meiosis during which DNA is exchanged by homologous recombination. Primary spermatocytes undergo two meiotic divisions to form haploid spermatids. Haploid spermatids differentiate through the elongation phase and eventually form mature spermatozoa through dramatic morphological changes termed spermiogenesis, during which: 1) the Golgi apparatus forms the acrosome, 2) nuclear chromatin undergoes compaction and condensation, 3) sperm tail is formed and 4) the excess cytoplasm of spermatid is eliminated. Given the complexity of spermatogenesis, the normal development of male germ cells is controlled by both protein-coding genes and small non-coding RNAs. Because of the lack of in vitro models, mouse models are currently the most powerful tools used to study these processes.KLHL10 (Kelch-like 10) is a spermatid-specific protein, which belongs to a BTB (Brica-brac, Tramtrack, and Broad-Complex)-Kelch protein superfamily. Haploinsufficiency of Klhl10 causes male infertility and prevents the genetic transmission of both mutant and wild-type alleles in mice. Therefore, a transgenic rescue strategy was used to overcome the haploinsufficiency of Klhl10 and knockout (KO) mice were generated for studying the function of Klhl10 during spermatogenesis. Klhl10 KO testes exhibited disrupted spermatogenesis, characterized by severe depletion of germ cells, degeneration of spermatids and reduction in the number of late spermatids. In Klhl10 KO testes, spermatid differentiation was arrested in elongating stage at step 9. Comparison of protein profiles between control and KO testes revealed that many mitochondrial proteins were up-regulated in Klhl10 KO testes. In addition, COX IV (mitochondrion marker) staining showed enhanced mitochondria signal in KLHL10 deple…
abstract: Spermatogenesis is a complex process that starts with the proliferation of differentiated spermatogonia through mitotic division. Primary spermatocytes produced from differentiated spermatogonia enter the prolonged prophase of meiosis during which DNA is exchanged by homologous recombination. Primary spermatocytes undergo two meiotic divisions to form haploid spermatids. Haploid spermatids differentiate through the elongation phase and eventually form mature spermatozoa through dramatic morphological changes termed spermiogenesis, during which: 1) the Golgi apparatus forms the acrosome, 2) nuclear chromatin undergoes compaction and condensation, 3) sperm tail is formed and 4) the excess cytoplasm of spermatid is eliminated. Given the complexity of spermatogenesis, the normal development of male germ cells is controlled by both protein-coding genes and small non-coding RNAs. Because of the lack of in vitro models, mouse models are currently the most powerful tools used to study these processes.KLHL10 (Kelch-like 10) is a spermatid-specific protein, which belongs to a BTB (Brica-brac, Tramtrack, and Broad-Complex)-Kelch protein superfamily. Haploinsufficiency of Klhl10 causes male infertility and prevents the genetic transmission of both mutant and wild-type alleles in mice. Therefore, a transgenic rescue strategy was used to overcome the haploinsufficiency of Klhl10 and knockout (KO) mice were generated for studying the function of Klhl10 during spermatogenesis. Klhl10 KO testes exhibited disrupted spermatogenesis, characterized by severe depletion of germ cells, degeneration of spermatids and reduction in the number of late spermatids. In Klhl10 KO testes, spermatid differentiation was arrested in elongating stage at step 9. Comparison of protein profiles between control and KO testes revealed that many mitochondrial proteins were up-regulated in Klhl10 KO testes. In addition, COX IV (mitochondrion marker) staining showed enhanced mitochondria signal in KLHL10 deple…
author: Wu, Qiuxia
date published: 2012
date modified: 2026-07-27T21:46:41Z
provider: University of Nevada - Reno
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Nevada - Reno

PropertyValue
property ID: Handle
value: http://hdl.handle.net/11714/3599