Exploring the Mitigation of TDP-43 Toxicity by Sis1 in Yeast

Amyotrophic Lateral Sclerosis is a progressive neurodegenerative disease with a 100% fatality rate. Most cases have no known cause, with 5-10% of cases having a familial link. Currently there is no cure. Research has revealed that Tar DNA-binding Protein 43 cytoplasmic aggregates are involved in many cases of Amyotrophic Lateral Sclerosis. One possible mechanism for TDP-43 induced toxicity is that these aggregates are titrating away a molecular chaperone protein, Sis1, from the nucleus of the cell. Sis1 is important in the degradation of proteins, and without it, the cell would become non-viable. In order to examine the relationship between the two proteins, they were tagged with fluorescent proteins and examined for colocalization under fluorescent microscopy. However, while TDP-43 does appear to titrate Sis1 out of the nucleus, there was no evidence that the two proteins colocalized.

Name thesis:degree_name

Biochemistry and Molecular Biology

Level thesis:degree_level

Honors Thesis

Grantor

University of Nevada, Reno

Year dc:date.issued

2016

Author dc:creator

• Ham, Jacqueline

Advisors dc:contributor.advisor

• Park, Sei-Kyoung
• Liebman, Susan

Statement dc:rights

• In Copyright

Language dc:language.iso

en_US, English

Handle dc:identifier.uri

http://hdl.handle.net/11714/3282

OAI identifier oai:identifier

oai:scholarwolf.unr.edu:11714/3282