{"id":{"repo_id":"unr","oai_identifier":"oai:scholarwolf.unr.edu:11714/2847"},"canonical_url":"https://search.dev.ndltd.org/etd/unr/oai:scholarwolf.unr.edu:11714/2847","repository":{"repo_id":"unr","name":"University of Nevada - Reno","base_url":"https://scholarwolf.unr.edu/server/oai/request"},"display":{"title":"Targeting Proteins of Aspergillus fumigatus for Monoclonal Antibody Production","abstract":"IA is increasingly prevalent and problematic among patients with immunodeficiency. Early diagnosis of IA can be difficult because the symptoms patients display are not specific to fungal infections, and the diagnostic tests currently in use are unreliable. It is well known that the organisms that cause IA shed protein antigens into body fluids once inside the host. In previous studies, our lab detected PPIase, Eglc, Ecm33, TrxR and CatB in IA mouse serum and urine, using an antigen discovery platform. In our current work, we seek to develop mAbs that specifically target PPIase, Eglc, Ecm33, TrxR and CatB as an effective diagnostic tool for the invasive fungus Aspergillus fumigatus. rPPIase, rEglC and rCatB were purified from bacterial cultures. rPPIase and rCatB were emulsified in TiterMax® Gold adjuvant (CytRx Corporation, Los Angeles, CA) and used to immunize BALB/c mice. The rPPIase emulsion elicited a robust immune response (high titers) which was confirmed via ELISA. The rPPIase immunized mice that had the highest titers were then boosted with recombinant protein in preparation for a splenocyte and myeloma fusion to produce a hybridoma cell line. A mAb was then selected and derived with high specificity and sensitivity for rPPIase. The long term goal is to produce high quality anti-PPIase, anti-Eglc, anti-Ecm33, anti-TrxR and anti-CatB mAbs as candidates for IA diagnostic tests, and possibly as a therapeutic option for IA patients.","abstract_html":"IA is increasingly prevalent and problematic among patients with immunodeficiency. Early diagnosis of IA can be difficult because the symptoms patients display are not specific to fungal infections, and the diagnostic tests currently in use are unreliable. It is well known that the organisms that cause IA shed protein antigens into body fluids once inside the host. In previous studies, our lab detected PPIase, Eglc, Ecm33, TrxR and CatB in IA mouse serum and urine, using an antigen discovery platform. In our current work, we seek to develop mAbs that specifically target PPIase, Eglc, Ecm33, TrxR and CatB as an effective diagnostic tool for the invasive fungus Aspergillus fumigatus. rPPIase, rEglC and rCatB were purified from bacterial cultures. rPPIase and rCatB were emulsified in TiterMax® Gold adjuvant (CytRx Corporation, Los Angeles, CA) and used to immunize BALB/c mice. The rPPIase emulsion elicited a robust immune response (high titers) which was confirmed via ELISA. The rPPIase immunized mice that had the highest titers were then boosted with recombinant protein in preparation for a splenocyte and myeloma fusion to produce a hybridoma cell line. A mAb was then selected and derived with high specificity and sensitivity for rPPIase. The long term goal is to produce high quality anti-PPIase, anti-Eglc, anti-Ecm33, anti-TrxR and anti-CatB mAbs as candidates for IA diagnostic tests, and possibly as a therapeutic option for IA patients.","abstract_has_math":false,"creators":["Moschetti, Anthony S."],"institution":null,"degree_name":null,"degree_level":"Master's Degree","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Kozel, Thomas R."],"committee_chairs":[],"committee_members":["Kozel, Thomas R.","AuCoin, David P.","Shintani, David K."],"year":2014,"date_issued":"2014","date_published":"2014","updated_at":"2026-07-27T21:47:36Z","subjects":["Aspergillus fumigatus","Fungal Diagnostics","Monoclonal Antibody","Peptidyl prolyl cis-trans isomerase"],"languages":[],"rights":["In Copyright(All Rights Reserved)"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/11714/2847","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Kozel, Thomas R."]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Kozel, Thomas R.","AuCoin, David P.","Shintani, David K."]},{"key":"dc:creator","label":"Author","values":["Moschetti, Anthony S."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2018-03-29T20:03:36Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2018-03-29T20:03:36Z"]},{"key":"dc:date.issued","label":"Date","values":["2014"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Master's Degree"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Aspergillus fumigatus","Fungal Diagnostics","Monoclonal Antibody","Peptidyl prolyl cis-trans isomerase"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["In Copyright(All Rights Reserved)"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/11714/2847"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["IA is increasingly prevalent and problematic among patients with immunodeficiency. Early diagnosis of IA can be difficult because the symptoms patients display are not specific to fungal infections, and the diagnostic tests currently in use are unreliable. It is well known that the organisms that cause IA shed protein antigens into body fluids once inside the host. In previous studies, our lab detected PPIase, Eglc, Ecm33, TrxR and CatB in IA mouse serum and urine, using an antigen discovery platform. In our current work, we seek to develop mAbs that specifically target PPIase, Eglc, Ecm33, TrxR and CatB as an effective diagnostic tool for the invasive fungus Aspergillus fumigatus. rPPIase, rEglC and rCatB were purified from bacterial cultures. rPPIase and rCatB were emulsified in TiterMax® Gold adjuvant (CytRx Corporation, Los Angeles, CA) and used to immunize BALB/c mice. The rPPIase emulsion elicited a robust immune response (high titers) which was confirmed via ELISA. The rPPIase immunized mice that had the highest titers were then boosted with recombinant protein in preparation for a splenocyte and myeloma fusion to produce a hybridoma cell line. A mAb was then selected and derived with high specificity and sensitivity for rPPIase. The long term goal is to produce high quality anti-PPIase, anti-Eglc, anti-Ecm33, anti-TrxR and anti-CatB mAbs as candidates for IA diagnostic tests, and possibly as a therapeutic option for IA patients."]},{"key":"dc:format","label":"Dc Format","values":["PDF"]},{"key":"dc:title","label":"Title","values":["Targeting Proteins of Aspergillus fumigatus for Monoclonal Antibody Production"]}]}],"canonical_facts":{"dc:contributor.advisor":["Kozel, Thomas R."],"dc:contributor.committeemember":["Kozel, Thomas R.","AuCoin, David P.","Shintani, David K."],"dc:creator":["Moschetti, Anthony S."],"dc:date.accessioned":["2018-03-29T20:03:36Z"],"dc:date.available":["2018-03-29T20:03:36Z"],"dc:date.issued":["2014"],"dc:description.abstract":["IA is increasingly prevalent and problematic among patients with immunodeficiency. Early diagnosis of IA can be difficult because the symptoms patients display are not specific to fungal infections, and the diagnostic tests currently in use are unreliable. It is well known that the organisms that cause IA shed protein antigens into body fluids once inside the host. In previous studies, our lab detected PPIase, Eglc, Ecm33, TrxR and CatB in IA mouse serum and urine, using an antigen discovery platform. In our current work, we seek to develop mAbs that specifically target PPIase, Eglc, Ecm33, TrxR and CatB as an effective diagnostic tool for the invasive fungus Aspergillus fumigatus. rPPIase, rEglC and rCatB were purified from bacterial cultures. rPPIase and rCatB were emulsified in TiterMax® Gold adjuvant (CytRx Corporation, Los Angeles, CA) and used to immunize BALB/c mice. The rPPIase emulsion elicited a robust immune response (high titers) which was confirmed via ELISA. The rPPIase immunized mice that had the highest titers were then boosted with recombinant protein in preparation for a splenocyte and myeloma fusion to produce a hybridoma cell line. A mAb was then selected and derived with high specificity and sensitivity for rPPIase. The long term goal is to produce high quality anti-PPIase, anti-Eglc, anti-Ecm33, anti-TrxR and anti-CatB mAbs as candidates for IA diagnostic tests, and possibly as a therapeutic option for IA patients."],"dc:format":["PDF"],"dc:identifier.uri":["http://hdl.handle.net/11714/2847"],"dc:rights":["In Copyright(All Rights Reserved)"],"dc:subject":["Aspergillus fumigatus","Fungal Diagnostics","Monoclonal Antibody","Peptidyl prolyl cis-trans isomerase"],"dc:title":["Targeting Proteins of Aspergillus fumigatus for Monoclonal Antibody Production"],"dc:type":["Thesis"],"thesis:degree_level":["Master's Degree"]},"updated_at":"2026-07-27T21:47:36Z"}