{"id":{"repo_id":"unr","oai_identifier":"oai:scholarwolf.unr.edu:11714/1920"},"canonical_url":"https://search.dev.ndltd.org/etd/unr/oai:scholarwolf.unr.edu:11714/1920","repository":{"repo_id":"unr","name":"University of Nevada - Reno","base_url":"https://scholarwolf.unr.edu/server/oai/request"},"display":{"title":"Tangential Flow Filtration for the Purification of Lucentis","abstract":"The goal of the Genentech Capstone project is to design a process to manufacture and purify a \"Lucentis®-like\" (Lu-L) protein. This group has been working on the tangential flow filtration (TFF) portion of the project, which will be utilized multiple times throughout the separation and purification process. The primary purpose of tangential flow filtration is to exchange buffer solution. Exchanging buffer solution regulates the pH of the mixture in preparation for chromatography, and changes the mobile phase utilized in each different chromatography step. The secondary purpose is to remove impurities from the buffer, while keeping the desired product on the upstream side of the membrane. Finally, tangential flow filtration can be used to concentrate proteins, which is an important step in the creation of the Lu-L product, as it allows for a product with the necessary specifications of volume, concentration, and purity. The team conducted trials to concentrate the modeling analog, hemoglobin, to observe the anticipated behavior of the Lu-L protein throughout TFF. In addition to performing those trials, the as well as modeling the industrial scale up of the TFF process will prepare students to start working with the Genentech protein and create the optimized scale-up system for the Lu-L protein.","abstract_html":"The goal of the Genentech Capstone project is to design a process to manufacture and purify a "Lucentis®-like" (Lu-L) protein. This group has been working on the tangential flow filtration (TFF) portion of the project, which will be utilized multiple times throughout the separation and purification process. The primary purpose of tangential flow filtration is to exchange buffer solution. Exchanging buffer solution regulates the pH of the mixture in preparation for chromatography, and changes the mobile phase utilized in each different chromatography step. The secondary purpose is to remove impurities from the buffer, while keeping the desired product on the upstream side of the membrane. Finally, tangential flow filtration can be used to concentrate proteins, which is an important step in the creation of the Lu-L product, as it allows for a product with the necessary specifications of volume, concentration, and purity. The team conducted trials to concentrate the modeling analog, hemoglobin, to observe the anticipated behavior of the Lu-L protein throughout TFF. In addition to performing those trials, the as well as modeling the industrial scale up of the TFF process will prepare students to start working with the Genentech protein and create the optimized scale-up system for the Lu-L protein.","abstract_has_math":false,"creators":["Chow, Genica I."],"institution":"University of Nevada, Reno","degree_name":"Chemical Engineering","degree_level":"Honors Thesis","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Fuchs, Alan W.","Hyland, Phillip L."],"committee_chairs":[],"committee_members":[],"year":2017,"date_issued":"2017","date_published":"2017","updated_at":"2026-07-27T21:45:34Z","subjects":[],"languages":["en_US","English"],"rights":["Creative Commons Attribution-NonCommercial-ShareAlike 4.0 United States"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/11714/1920","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Fuchs, Alan W.","Hyland, Phillip L."]},{"key":"dc:creator","label":"Author","values":["Chow, Genica I."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2017-08-31T21:17:43Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2017-08-31T21:17:43Z"]},{"key":"dc:date.issued","label":"Date","values":["2017"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Honors Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Chemical Engineering"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Nevada, Reno"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["English"]},{"key":"dc:language.iso","label":"Language (ISO)","values":["en_US"]},{"key":"dc:rights","label":"Dc Rights","values":["Creative Commons Attribution-NonCommercial-ShareAlike 4.0 United States"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/11714/1920"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The University of Nevada, Reno Libraries will promptly respond to removal requests related to content that violates intellectual property laws, data protections, or has been uploaded without creator consent. Takedown notices should be directed to our ScholarWolf team (scholarwolf@library.unr.edu) with information about the object, including its full URL and the nature of your complaint."]},{"key":"dc:description.abstract","label":"Abstract","values":["The goal of the Genentech Capstone project is to design a process to manufacture and purify a \"Lucentis®-like\" (Lu-L) protein. This group has been working on the tangential flow filtration (TFF) portion of the project, which will be utilized multiple times throughout the separation and purification process. The primary purpose of tangential flow filtration is to exchange buffer solution. Exchanging buffer solution regulates the pH of the mixture in preparation for chromatography, and changes the mobile phase utilized in each different chromatography step. The secondary purpose is to remove impurities from the buffer, while keeping the desired product on the upstream side of the membrane. Finally, tangential flow filtration can be used to concentrate proteins, which is an important step in the creation of the Lu-L product, as it allows for a product with the necessary specifications of volume, concentration, and purity. The team conducted trials to concentrate the modeling analog, hemoglobin, to observe the anticipated behavior of the Lu-L protein throughout TFF. In addition to performing those trials, the as well as modeling the industrial scale up of the TFF process will prepare students to start working with the Genentech protein and create the optimized scale-up system for the Lu-L protein."]},{"key":"dc:format","label":"Dc Format","values":["PDF"]},{"key":"dc:title","label":"Title","values":["Tangential Flow Filtration for the Purification of Lucentis"]}]}],"canonical_facts":{"dc:contributor.advisor":["Fuchs, Alan W.","Hyland, Phillip L."],"dc:creator":["Chow, Genica I."],"dc:date.accessioned":["2017-08-31T21:17:43Z"],"dc:date.available":["2017-08-31T21:17:43Z"],"dc:date.issued":["2017"],"dc:description":["The University of Nevada, Reno Libraries will promptly respond to removal requests related to content that violates intellectual property laws, data protections, or has been uploaded without creator consent. Takedown notices should be directed to our ScholarWolf team (scholarwolf@library.unr.edu) with information about the object, including its full URL and the nature of your complaint."],"dc:description.abstract":["The goal of the Genentech Capstone project is to design a process to manufacture and purify a \"Lucentis®-like\" (Lu-L) protein. This group has been working on the tangential flow filtration (TFF) portion of the project, which will be utilized multiple times throughout the separation and purification process. The primary purpose of tangential flow filtration is to exchange buffer solution. Exchanging buffer solution regulates the pH of the mixture in preparation for chromatography, and changes the mobile phase utilized in each different chromatography step. The secondary purpose is to remove impurities from the buffer, while keeping the desired product on the upstream side of the membrane. Finally, tangential flow filtration can be used to concentrate proteins, which is an important step in the creation of the Lu-L product, as it allows for a product with the necessary specifications of volume, concentration, and purity. The team conducted trials to concentrate the modeling analog, hemoglobin, to observe the anticipated behavior of the Lu-L protein throughout TFF. In addition to performing those trials, the as well as modeling the industrial scale up of the TFF process will prepare students to start working with the Genentech protein and create the optimized scale-up system for the Lu-L protein."],"dc:format":["PDF"],"dc:identifier.uri":["http://hdl.handle.net/11714/1920"],"dc:language":["English"],"dc:language.iso":["en_US"],"dc:rights":["Creative Commons Attribution-NonCommercial-ShareAlike 4.0 United States"],"dc:title":["Tangential Flow Filtration for the Purification of Lucentis"],"dc:type":["Thesis"],"thesis:degree_level":["Honors Thesis"],"thesis:degree_name":["Chemical Engineering"],"thesis:institution_name":["University of Nevada, Reno"]},"updated_at":"2026-07-27T21:45:34Z"}