{"id":{"repo_id":"unr","oai_identifier":"oai:scholarwolf.unr.edu:11714/1897"},"canonical_url":"https://search.dev.ndltd.org/etd/unr/oai:scholarwolf.unr.edu:11714/1897","repository":{"repo_id":"unr","name":"University of Nevada - Reno","base_url":"https://scholarwolf.unr.edu/server/oai/request"},"display":{"title":"Genentech: Purification of Monoclonal Antibody","abstract":"Lucentis, a monoclonal antibody fragment produced in Escherichia coli, is used to treat wet age-related macular degeneration, one of the leading causes of legal blindness. The objective of this thesis is to analyze the process of antibody fragment separation and to purify a solution of \"Lucentis like\" protein provided by Genentech to a 99% purity. This paper focuses on the chromatography aspect of the process and investigates the multiple types of chromatography used in both the base case and alternative case presented with a focus on cation exchange chromatography. The project was completed over an eight-month time period spanning two semesters. The team studied various theories, design models, performance equations,, chromatography techniques, literature reviews, and economic analyses. The results obtained from experimental analysis, and after consulting with various teams, instructors, and Genentech employees, highly suggested that the \"Lucentis like\" protein used was denature and no longer had the properties of the desired protein.","abstract_html":"Lucentis, a monoclonal antibody fragment produced in Escherichia coli, is used to treat wet age-related macular degeneration, one of the leading causes of legal blindness. The objective of this thesis is to analyze the process of antibody fragment separation and to purify a solution of "Lucentis like" protein provided by Genentech to a 99% purity. This paper focuses on the chromatography aspect of the process and investigates the multiple types of chromatography used in both the base case and alternative case presented with a focus on cation exchange chromatography. The project was completed over an eight-month time period spanning two semesters. The team studied various theories, design models, performance equations,, chromatography techniques, literature reviews, and economic analyses. The results obtained from experimental analysis, and after consulting with various teams, instructors, and Genentech employees, highly suggested that the "Lucentis like" protein used was denature and no longer had the properties of the desired protein.","abstract_has_math":false,"creators":["Salmon, Arielle"],"institution":"University of Nevada, Reno","degree_name":"Chemical Engineering","degree_level":"Honors Thesis","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Fuchs, Alan W."],"committee_chairs":[],"committee_members":[],"year":2017,"date_issued":"2017","date_published":"2017","updated_at":"2026-07-27T21:46:30Z","subjects":[],"languages":["en_US","English"],"rights":["In Copyright(All Rights Reserved)"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/11714/1897","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Fuchs, Alan W."]},{"key":"dc:creator","label":"Author","values":["Salmon, Arielle"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2017-08-31T21:17:40Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2017-08-31T21:17:40Z"]},{"key":"dc:date.issued","label":"Date","values":["2017"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Honors Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Chemical Engineering"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Nevada, Reno"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["English"]},{"key":"dc:language.iso","label":"Language (ISO)","values":["en_US"]},{"key":"dc:rights","label":"Dc Rights","values":["In Copyright(All Rights Reserved)"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/11714/1897"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The University of Nevada, Reno Libraries will promptly respond to removal requests related to content that violates intellectual property laws, data protections, or has been uploaded without creator consent. Takedown notices should be directed to our ScholarWolf team (scholarwolf@library.unr.edu) with information about the object, including its full URL and the nature of your complaint."]},{"key":"dc:description.abstract","label":"Abstract","values":["Lucentis, a monoclonal antibody fragment produced in Escherichia coli, is used to treat wet age-related macular degeneration, one of the leading causes of legal blindness. The objective of this thesis is to analyze the process of antibody fragment separation and to purify a solution of \"Lucentis like\" protein provided by Genentech to a 99% purity. This paper focuses on the chromatography aspect of the process and investigates the multiple types of chromatography used in both the base case and alternative case presented with a focus on cation exchange chromatography. The project was completed over an eight-month time period spanning two semesters. The team studied various theories, design models, performance equations,, chromatography techniques, literature reviews, and economic analyses. The results obtained from experimental analysis, and after consulting with various teams, instructors, and Genentech employees, highly suggested that the \"Lucentis like\" protein used was denature and no longer had the properties of the desired protein."]},{"key":"dc:format","label":"Dc Format","values":["PDF"]},{"key":"dc:title","label":"Title","values":["Genentech: Purification of Monoclonal Antibody"]}]}],"canonical_facts":{"dc:contributor.advisor":["Fuchs, Alan W."],"dc:creator":["Salmon, Arielle"],"dc:date.accessioned":["2017-08-31T21:17:40Z"],"dc:date.available":["2017-08-31T21:17:40Z"],"dc:date.issued":["2017"],"dc:description":["The University of Nevada, Reno Libraries will promptly respond to removal requests related to content that violates intellectual property laws, data protections, or has been uploaded without creator consent. Takedown notices should be directed to our ScholarWolf team (scholarwolf@library.unr.edu) with information about the object, including its full URL and the nature of your complaint."],"dc:description.abstract":["Lucentis, a monoclonal antibody fragment produced in Escherichia coli, is used to treat wet age-related macular degeneration, one of the leading causes of legal blindness. The objective of this thesis is to analyze the process of antibody fragment separation and to purify a solution of \"Lucentis like\" protein provided by Genentech to a 99% purity. This paper focuses on the chromatography aspect of the process and investigates the multiple types of chromatography used in both the base case and alternative case presented with a focus on cation exchange chromatography. The project was completed over an eight-month time period spanning two semesters. The team studied various theories, design models, performance equations,, chromatography techniques, literature reviews, and economic analyses. The results obtained from experimental analysis, and after consulting with various teams, instructors, and Genentech employees, highly suggested that the \"Lucentis like\" protein used was denature and no longer had the properties of the desired protein."],"dc:format":["PDF"],"dc:identifier.uri":["http://hdl.handle.net/11714/1897"],"dc:language":["English"],"dc:language.iso":["en_US"],"dc:rights":["In Copyright(All Rights Reserved)"],"dc:title":["Genentech: Purification of Monoclonal Antibody"],"dc:type":["Thesis"],"thesis:degree_level":["Honors Thesis"],"thesis:degree_name":["Chemical Engineering"],"thesis:institution_name":["University of Nevada, Reno"]},"updated_at":"2026-07-27T21:46:30Z"}