The CCAAT binding-factor (CBF) is a transcriptional activator conserved in eukaryotes. The CBF in Saccharomyces cerevisiae is a multimeric heteromer termed the Hap2/3/4/5 complex. Hap4, which contains the activation domain of the complex, is also the regulatory subunit and is known to be transcriptionally controlled by carbon sources. However, little is known about Hap4 regulation. In this report, I identify mechanisms by which Hap4 is regulated, including: (1) transcriptional regulation via two short upstream open reading frames (uORFs) in the 5' leader sequence of HAP4 mRNA; (2) proteasome-dependent degradation of Hap4; and (3) identification of two negative regulators of HAP4 expression, CYC8 and SIN4. I also report differential patterns of Hap4 cellular localization which depends on (1) carbon sources, (2) abundance of Hap4 protein, and (3) presence or absence of mitochondrial DNA (mtDNA).
","abstract_html":"<p>The CCAAT binding-factor (CBF) is a transcriptional activator conserved in eukaryotes. The CBF in Saccharomyces cerevisiae is a multimeric heteromer termed the Hap2/3/4/5 complex. Hap4, which contains the activation domain of the complex, is also the regulatory subunit and is known to be transcriptionally controlled by carbon sources. However, little is known about Hap4 regulation. In this report, I identify mechanisms by which Hap4 is regulated, including: (1) transcriptional regulation via two short upstream open reading frames (uORFs) in the 5' leader sequence of HAP4 mRNA; (2) proteasome-dependent degradation of Hap4; and (3) identification of two negative regulators of HAP4 expression, CYC8 and SIN4. I also report differential patterns of Hap4 cellular localization which depends on (1) carbon sources, (2) abundance of Hap4 protein, and (3) presence or absence of mitochondrial DNA (mtDNA).</p>","abstract_has_math":false,"creators":["Capps, Denise"],"institution":null,"degree_name":"M.S.","degree_level":"Thesis-Restricted","degree_discipline":"Biological Sciences","degree_department":null,"school":null,"contributors":["Liu, Zhengchang","Schluchter, Wendy","Clancy, Mary J."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-05-20T07:00:00Z","date_published":"2011-05-20T07:00:00Z","updated_at":"2026-07-24T05:28:04Z","subjects":["Hap2","Hap3","Hap4","Hap5","Hap2-5","CCAAT-binding factor","CBF","cerevisiae","SSN6","CYC8","SIN4","upstream open reading frames","uORF","glucose repression","carbon catabolite repression"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://scholarworks.uno.edu/td/116","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Liu, Zhengchang","Schluchter, Wendy","Clancy, Mary J."]},{"key":"dc:creator","label":"Author","values":["Capps, Denise"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"thesis:degree_discipline","label":"Discipline","values":["Biological Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis-Restricted"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Hap2","Hap3","Hap4","Hap5","Hap2-5","CCAAT-binding factor","CBF","cerevisiae","SSN6","CYC8","SIN4","upstream open reading frames","uORF","glucose repression","carbon catabolite repression"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://scholarworks.uno.edu/td/116"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The CCAAT binding-factor (CBF) is a transcriptional activator conserved in eukaryotes. The CBF in Saccharomyces cerevisiae is a multimeric heteromer termed the Hap2/3/4/5 complex. Hap4, which contains the activation domain of the complex, is also the regulatory subunit and is known to be transcriptionally controlled by carbon sources. However, little is known about Hap4 regulation. In this report, I identify mechanisms by which Hap4 is regulated, including: (1) transcriptional regulation via two short upstream open reading frames (uORFs) in the 5' leader sequence of HAP4 mRNA; (2) proteasome-dependent degradation of Hap4; and (3) identification of two negative regulators of HAP4 expression, CYC8 and SIN4. I also report differential patterns of Hap4 cellular localization which depends on (1) carbon sources, (2) abundance of Hap4 protein, and (3) presence or absence of mitochondrial DNA (mtDNA).
"]},{"key":"dc:title","label":"Title","values":["The Regulation of NAP4 in Saccharomyces cerevisiae"]}]}],"canonical_facts":{"dc:contributor":["Liu, Zhengchang","Schluchter, Wendy","Clancy, Mary J."],"dc:creator":["Capps, Denise"],"dc:description.abstract":["The CCAAT binding-factor (CBF) is a transcriptional activator conserved in eukaryotes. The CBF in Saccharomyces cerevisiae is a multimeric heteromer termed the Hap2/3/4/5 complex. Hap4, which contains the activation domain of the complex, is also the regulatory subunit and is known to be transcriptionally controlled by carbon sources. However, little is known about Hap4 regulation. In this report, I identify mechanisms by which Hap4 is regulated, including: (1) transcriptional regulation via two short upstream open reading frames (uORFs) in the 5' leader sequence of HAP4 mRNA; (2) proteasome-dependent degradation of Hap4; and (3) identification of two negative regulators of HAP4 expression, CYC8 and SIN4. I also report differential patterns of Hap4 cellular localization which depends on (1) carbon sources, (2) abundance of Hap4 protein, and (3) presence or absence of mitochondrial DNA (mtDNA).
"],"dc:identifier":["https://scholarworks.uno.edu/td/116"],"dc:subject":["Hap2","Hap3","Hap4","Hap5","Hap2-5","CCAAT-binding factor","CBF","cerevisiae","SSN6","CYC8","SIN4","upstream open reading frames","uORF","glucose repression","carbon catabolite repression"],"dc:title":["The Regulation of NAP4 in Saccharomyces cerevisiae"],"thesis:degree_discipline":["Biological Sciences"],"thesis:degree_level":["Thesis-Restricted"],"thesis:degree_name":["M.S."]},"updated_at":"2026-07-24T05:28:04Z"}