{"id":{"repo_id":"unm","oai_identifier":"oai:digitalrepository.unm.edu:ose_etds-1035"},"canonical_url":"https://search.dev.ndltd.org/etd/unm/oai:digitalrepository.unm.edu:ose_etds-1035","repository":{"repo_id":"unm","name":"University of New Mexico","base_url":"https://digitalrepository.unm.edu/do/oai/"},"display":{"title":"An automated and user-friendly optical tweezers for biomolecular investigations","abstract":"Optical tweezers are not new to biological applications but building a tweezers which is versatile, automated, precise, stable and user-friendly is quite challenging. Such a tweezers usually require not only a hefty budget but vast understanding of engineer- ing of optomechanical systems. I describe in this dissertation a versatile, automated, precise, stable and user-friendly optical tweezers that we developed for biomolecular investigations under limited budget. I describe automation and control improvements along with newly-developed surface detection software. I also describe cost-e ective optomechanical devices used in tweezers with potential for broader application. Fi- nally, I present new results of single-molecule force spectroscopy of DNA overstretch- ing as a function of water isotope type. Single DNA unzipping is also presented as a proof of concept.","abstract_html":"Optical tweezers are not new to biological applications but building a tweezers which is versatile, automated, precise, stable and user-friendly is quite challenging. Such a tweezers usually require not only a hefty budget but vast understanding of engineer- ing of optomechanical systems. I describe in this dissertation a versatile, automated, precise, stable and user-friendly optical tweezers that we developed for biomolecular investigations under limited budget. I describe automation and control improvements along with newly-developed surface detection software. I also describe cost-e ective optomechanical devices used in tweezers with potential for broader application. Fi- nally, I present new results of single-molecule force spectroscopy of DNA overstretch- ing as a function of water isotope type. Single DNA unzipping is also presented as a proof of concept.","abstract_has_math":false,"creators":["rahti, pranav"],"institution":null,"degree_name":"Optical Science and Engineering","degree_level":"Doctoral","degree_discipline":"Optical Science and Engineering","degree_department":null,"school":null,"contributors":["koch, steven","Lester, Luke","Thomas, james","Lidke, Keith"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2013,"date_issued":"2013-09-05T07:00:00Z","date_published":"2013-09-05T07:00:00Z","updated_at":"2026-07-24T05:26:35Z","subjects":[],"languages":["English"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://digitalrepository.unm.edu/ose_etds/36"],"render_values":[{"text":"https://digitalrepository.unm.edu/ose_etds/36","href":"https://digitalrepository.unm.edu/ose_etds/36","code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/1928/23366","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["koch, steven","Lester, Luke","Thomas, james","Lidke, Keith"]},{"key":"dc:creator","label":"Author","values":["rahti, pranav"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"thesis:degree_discipline","label":"Discipline","values":["Optical Science and Engineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Doctoral","Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Optical Science and Engineering"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["English"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/1928/23366","https://digitalrepository.unm.edu/ose_etds/36"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Optical tweezers are not new to biological applications but building a tweezers which is versatile, automated, precise, stable and user-friendly is quite challenging. Such a tweezers usually require not only a hefty budget but vast understanding of engineer- ing of optomechanical systems. I describe in this dissertation a versatile, automated, precise, stable and user-friendly optical tweezers that we developed for biomolecular investigations under limited budget. I describe automation and control improvements along with newly-developed surface detection software. I also describe cost-e ective optomechanical devices used in tweezers with potential for broader application. Fi- nally, I present new results of single-molecule force spectroscopy of DNA overstretch- ing as a function of water isotope type. 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I also describe cost-e ective optomechanical devices used in tweezers with potential for broader application. Fi- nally, I present new results of single-molecule force spectroscopy of DNA overstretch- ing as a function of water isotope type. Single DNA unzipping is also presented as a proof of concept."],"dc:identifier":["http://hdl.handle.net/1928/23366","https://digitalrepository.unm.edu/ose_etds/36"],"dc:language":["English"],"dc:title":["An automated and user-friendly optical tweezers for biomolecular investigations"],"thesis:degree_discipline":["Optical Science and Engineering"],"thesis:degree_level":["Doctoral","Dissertation"],"thesis:degree_name":["Optical Science and Engineering"]},"updated_at":"2026-07-24T05:26:35Z"}