{"id":{"repo_id":"unm","oai_identifier":"oai:digitalrepository.unm.edu:educ_hess_etds-1012"},"canonical_url":"https://search.dev.ndltd.org/etd/unm/oai:digitalrepository.unm.edu:educ_hess_etds-1012","repository":{"repo_id":"unm","name":"University of New Mexico","base_url":"https://digitalrepository.unm.edu/do/oai/"},"display":{"title":"Sex Differences in Heat Shock Protein 72 Expression and Inflammatory Response to Acute Exercise in the Heat.","abstract":"This study evaluated possible sex differences in intracellular heat shock protein 72 (Hsp72), intracellular cytokines, and extracellular Hsp72 (eHsp72) before and after exercise in the heat. Nine non-heat acclimated women (W) (age 23 ± 3, BF 21 ± 2%, VO2max 58 ± 5 ml/kgFFM/min) and nine non-heat acclimated men (M) (age 25 ± 5, BF 12 ± 5%, VO2max 60 ± 7 ml/kgFFM/min) completed 2 treadmill bouts at 60% VO2max for 60 min in a 42°C, 20% RH environment. The W had normal menstrual cycles and were tested in counterbalanced order during follicular (fol) and luteal (lut) phases. M and Ws duplicate trials were separated by 12 ± 2 days. Blood samples were drawn pre, 0, 1, and 4 hrs post-exercise. Mononucleated cells were analyzed for Hsp72, IL-1ra, IL-6, and TNF-α using flow cytometry. eHsp72 was analyzed using ELISA. In trial 1, Hsp72 content increased in M by 37% 4 hrs post exercise (p<0.05), but did not change significantly in W at any time after exercise. When Hsp72 expression was normalized to baseline, M expressed greater Hsp72 than W (p<0.05) after exercise. Baseline Hsp72 increased by 26% in M from trial 1 to trial 2 (p<0.05), but this effect did not occur in W. eHsp72 did not change after exercise, but there was a main effect for M having higher levels than W (p<0.05). While cytokines did not change during exercise, W consistently expressed less IL-1ra than M (p<0.05). IL-6 and TNF-α were higher in the fol than lut phase at 4 hrs post exercise (p<0.05). Our findings suggest that unacclimated M and W differ in their expression of Hsp72 and eHsp72 after exercise in the heat. M up-regulate Hsp 72 after a single bout of exercise in the heat, which persisted for 12 days, suggesting an acquired cellular thermotolerance. The inhibition of Hsp72 expression in W after exercise could be due to a known effect of estrogen to stabilize the cell membrane or to its action as an anti-oxidant.'","abstract_html":"This study evaluated possible sex differences in intracellular heat shock protein 72 (Hsp72), intracellular cytokines, and extracellular Hsp72 (eHsp72) before and after exercise in the heat. Nine non-heat acclimated women (W) (age 23 &#177; 3, BF 21 &#177; 2%, VO2max 58 &#177; 5 ml/kgFFM/min) and nine non-heat acclimated men (M) (age 25 &#177; 5, BF 12 &#177; 5%, VO2max 60 &#177; 7 ml/kgFFM/min) completed 2 treadmill bouts at 60% VO2max for 60 min in a 42&#176;C, 20% RH environment. The W had normal menstrual cycles and were tested in counterbalanced order during follicular (fol) and luteal (lut) phases. M and Ws duplicate trials were separated by 12 &#177; 2 days. Blood samples were drawn pre, 0, 1, and 4 hrs post-exercise. Mononucleated cells were analyzed for Hsp72, IL-1ra, IL-6, and TNF-&#945; using flow cytometry. eHsp72 was analyzed using ELISA. In trial 1, Hsp72 content increased in M by 37% 4 hrs post exercise (p<0.05), but did not change significantly in W at any time after exercise. When Hsp72 expression was normalized to baseline, M expressed greater Hsp72 than W (p<0.05) after exercise. Baseline Hsp72 increased by 26% in M from trial 1 to trial 2 (p<0.05), but this effect did not occur in W. eHsp72 did not change after exercise, but there was a main effect for M having higher levels than W (p<0.05). While cytokines did not change during exercise, W consistently expressed less IL-1ra than M (p<0.05). IL-6 and TNF-&#945; were higher in the fol than lut phase at 4 hrs post exercise (p<0.05). Our findings suggest that unacclimated M and W differ in their expression of Hsp72 and eHsp72 after exercise in the heat. M up-regulate Hsp 72 after a single bout of exercise in the heat, which persisted for 12 days, suggesting an acquired cellular thermotolerance. The inhibition of Hsp72 expression in W after exercise could be due to a known effect of estrogen to stabilize the cell membrane or to its action as an anti-oxidant.'","abstract_has_math":false,"creators":["Gillum, Trevor"],"institution":null,"degree_name":"Physical Education, Sports and Exercise Science","degree_level":"Dissertation","degree_discipline":"Health, Exercise, and Sports Sciences","degree_department":null,"school":null,"contributors":["Schneider, Suzanne","Kravitz, Len","Mermier, Christine","Moseley, Pope"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2010,"date_issued":"2010-09-09T07:00:00Z","date_published":"2010-09-09T07:00:00Z","updated_at":"2026-07-24T05:26:22Z","subjects":["Exercise--Physiological aspects--Sex differences","Body temperature--Regulation--Sex differences","Heat shock proteins--Effect of heat on","Cytokines"],"languages":["English"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://digitalrepository.unm.edu/educ_hess_etds/13"],"render_values":[{"text":"https://digitalrepository.unm.edu/educ_hess_etds/13","href":"https://digitalrepository.unm.edu/educ_hess_etds/13","code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/1928/11145","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Schneider, Suzanne","Kravitz, Len","Mermier, Christine","Moseley, Pope"]},{"key":"dc:creator","label":"Author","values":["Gillum, Trevor"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"thesis:degree_discipline","label":"Discipline","values":["Health, Exercise, and Sports Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation","Doctoral"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Physical Education, Sports and Exercise Science"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Exercise--Physiological aspects--Sex differences","Body temperature--Regulation--Sex differences","Heat shock proteins--Effect of heat on","Cytokines"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["English"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/1928/11145","https://digitalrepository.unm.edu/educ_hess_etds/13"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["This study evaluated possible sex differences in intracellular heat shock protein 72 (Hsp72), intracellular cytokines, and extracellular Hsp72 (eHsp72) before and after exercise in the heat. Nine non-heat acclimated women (W) (age 23 ± 3, BF 21 ± 2%, VO2max 58 ± 5 ml/kgFFM/min) and nine non-heat acclimated men (M) (age 25 ± 5, BF 12 ± 5%, VO2max 60 ± 7 ml/kgFFM/min) completed 2 treadmill bouts at 60% VO2max for 60 min in a 42°C, 20% RH environment. The W had normal menstrual cycles and were tested in counterbalanced order during follicular (fol) and luteal (lut) phases. M and Ws duplicate trials were separated by 12 ± 2 days. Blood samples were drawn pre, 0, 1, and 4 hrs post-exercise. Mononucleated cells were analyzed for Hsp72, IL-1ra, IL-6, and TNF-α using flow cytometry. eHsp72 was analyzed using ELISA. In trial 1, Hsp72 content increased in M by 37% 4 hrs post exercise (p<0.05), but did not change significantly in W at any time after exercise. When Hsp72 expression was normalized to baseline, M expressed greater Hsp72 than W (p<0.05) after exercise. Baseline Hsp72 increased by 26% in M from trial 1 to trial 2 (p<0.05), but this effect did not occur in W. eHsp72 did not change after exercise, but there was a main effect for M having higher levels than W (p<0.05). While cytokines did not change during exercise, W consistently expressed less IL-1ra than M (p<0.05). IL-6 and TNF-α were higher in the fol than lut phase at 4 hrs post exercise (p<0.05). Our findings suggest that unacclimated M and W differ in their expression of Hsp72 and eHsp72 after exercise in the heat. M up-regulate Hsp 72 after a single bout of exercise in the heat, which persisted for 12 days, suggesting an acquired cellular thermotolerance. The inhibition of Hsp72 expression in W after exercise could be due to a known effect of estrogen to stabilize the cell membrane or to its action as an anti-oxidant.'"]},{"key":"dc:title","label":"Title","values":["Sex Differences in Heat Shock Protein 72 Expression and Inflammatory Response to Acute Exercise in the Heat."]}]}],"canonical_facts":{"dc:contributor":["Schneider, Suzanne","Kravitz, Len","Mermier, Christine","Moseley, Pope"],"dc:creator":["Gillum, Trevor"],"dc:description.abstract":["This study evaluated possible sex differences in intracellular heat shock protein 72 (Hsp72), intracellular cytokines, and extracellular Hsp72 (eHsp72) before and after exercise in the heat. Nine non-heat acclimated women (W) (age 23 ± 3, BF 21 ± 2%, VO2max 58 ± 5 ml/kgFFM/min) and nine non-heat acclimated men (M) (age 25 ± 5, BF 12 ± 5%, VO2max 60 ± 7 ml/kgFFM/min) completed 2 treadmill bouts at 60% VO2max for 60 min in a 42°C, 20% RH environment. The W had normal menstrual cycles and were tested in counterbalanced order during follicular (fol) and luteal (lut) phases. M and Ws duplicate trials were separated by 12 ± 2 days. Blood samples were drawn pre, 0, 1, and 4 hrs post-exercise. Mononucleated cells were analyzed for Hsp72, IL-1ra, IL-6, and TNF-α using flow cytometry. eHsp72 was analyzed using ELISA. In trial 1, Hsp72 content increased in M by 37% 4 hrs post exercise (p<0.05), but did not change significantly in W at any time after exercise. When Hsp72 expression was normalized to baseline, M expressed greater Hsp72 than W (p<0.05) after exercise. Baseline Hsp72 increased by 26% in M from trial 1 to trial 2 (p<0.05), but this effect did not occur in W. eHsp72 did not change after exercise, but there was a main effect for M having higher levels than W (p<0.05). While cytokines did not change during exercise, W consistently expressed less IL-1ra than M (p<0.05). IL-6 and TNF-α were higher in the fol than lut phase at 4 hrs post exercise (p<0.05). Our findings suggest that unacclimated M and W differ in their expression of Hsp72 and eHsp72 after exercise in the heat. M up-regulate Hsp 72 after a single bout of exercise in the heat, which persisted for 12 days, suggesting an acquired cellular thermotolerance. The inhibition of Hsp72 expression in W after exercise could be due to a known effect of estrogen to stabilize the cell membrane or to its action as an anti-oxidant.'"],"dc:identifier":["http://hdl.handle.net/1928/11145","https://digitalrepository.unm.edu/educ_hess_etds/13"],"dc:language":["English"],"dc:subject":["Exercise--Physiological aspects--Sex differences","Body temperature--Regulation--Sex differences","Heat shock proteins--Effect of heat on","Cytokines"],"dc:title":["Sex Differences in Heat Shock Protein 72 Expression and Inflammatory Response to Acute Exercise in the Heat."],"thesis:degree_discipline":["Health, Exercise, and Sports Sciences"],"thesis:degree_level":["Dissertation","Doctoral"],"thesis:degree_name":["Physical Education, Sports and Exercise Science"]},"updated_at":"2026-07-24T05:26:22Z"}