{"id":{"repo_id":"unm","oai_identifier":"oai:digitalrepository.unm.edu:bme_etds-1001"},"canonical_url":"https://search.dev.ndltd.org/etd/unm/oai:digitalrepository.unm.edu:bme_etds-1001","repository":{"repo_id":"unm","name":"University of New Mexico","base_url":"https://digitalrepository.unm.edu/do/oai/"},"display":{"title":"MOL-PCR For SNP Based Detection and Characterization of STEC non-O157 Strains","abstract":"Shiga toxin-producing Escherichia coli (STEC) strains are important food-borne pathogens that have been linked to human illness. In the United States, STEC O157:H7 strains have been attributed to the majority of disease and outbreaks; however, the frequency of disease associated with STEC non-O157 strains have also increased and is often under recognized due to diagnostic limitations. In 2012, the Food Safety and Inspection Service (FSIS) implemented mandatory testing for non-O157 STEC serogroups O26, O45, O103, O111, O121, and O145. Single nucleotide polymorphisms (SNPs) have been identified in the O-antigen gene cluster for each of the non-O157 STEC serogroups that can differentiate between a STEC and non-STEC strain. In this study, the multiplex oligonucleotide ligation-PCR (MOL-PCR) detection platform was adapted for the detection and characterization of the above-described non-O157 STEC serogroups. The SNP-based MOL-PCR assay has demonstrated the ability to simultaneously detect the non-O157 serogroups and characterize the strain based on the SNP discriminates identified for each serogroup. This work provides a potential diagnostic platform that can reduce diagnostic limitations for the detection of non-O157 serogroups.","abstract_html":"Shiga toxin-producing Escherichia coli (STEC) strains are important food-borne pathogens that have been linked to human illness. In the United States, STEC O157:H7 strains have been attributed to the majority of disease and outbreaks; however, the frequency of disease associated with STEC non-O157 strains have also increased and is often under recognized due to diagnostic limitations. In 2012, the Food Safety and Inspection Service (FSIS) implemented mandatory testing for non-O157 STEC serogroups O26, O45, O103, O111, O121, and O145. Single nucleotide polymorphisms (SNPs) have been identified in the O-antigen gene cluster for each of the non-O157 STEC serogroups that can differentiate between a STEC and non-STEC strain. In this study, the multiplex oligonucleotide ligation-PCR (MOL-PCR) detection platform was adapted for the detection and characterization of the above-described non-O157 STEC serogroups. The SNP-based MOL-PCR assay has demonstrated the ability to simultaneously detect the non-O157 serogroups and characterize the strain based on the SNP discriminates identified for each serogroup. This work provides a potential diagnostic platform that can reduce diagnostic limitations for the detection of non-O157 serogroups.","abstract_has_math":false,"creators":["Mendez, Heather M."],"institution":null,"degree_name":"Biomedical Engineering","degree_level":"Thesis","degree_discipline":"Biomedical Engineering","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-11-13T08:00:00Z","date_published":"2015-11-13T08:00:00Z","updated_at":"2026-07-24T05:25:53Z","subjects":["Detection of STEC non-O157 Serogroups"],"languages":["English"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://digitalrepository.unm.edu/bme_etds/2"],"render_values":[{"text":"https://digitalrepository.unm.edu/bme_etds/2","href":"https://digitalrepository.unm.edu/bme_etds/2","code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/1928/31682","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Mendez, Heather M."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"thesis:degree_discipline","label":"Discipline","values":["Biomedical Engineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis","Masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Biomedical Engineering"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Detection of STEC non-O157 Serogroups"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["English"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/1928/31682","https://digitalrepository.unm.edu/bme_etds/2"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Shiga toxin-producing Escherichia coli (STEC) strains are important food-borne pathogens that have been linked to human illness. In the United States, STEC O157:H7 strains have been attributed to the majority of disease and outbreaks; however, the frequency of disease associated with STEC non-O157 strains have also increased and is often under recognized due to diagnostic limitations. In 2012, the Food Safety and Inspection Service (FSIS) implemented mandatory testing for non-O157 STEC serogroups O26, O45, O103, O111, O121, and O145. Single nucleotide polymorphisms (SNPs) have been identified in the O-antigen gene cluster for each of the non-O157 STEC serogroups that can differentiate between a STEC and non-STEC strain. In this study, the multiplex oligonucleotide ligation-PCR (MOL-PCR) detection platform was adapted for the detection and characterization of the above-described non-O157 STEC serogroups. The SNP-based MOL-PCR assay has demonstrated the ability to simultaneously detect the non-O157 serogroups and characterize the strain based on the SNP discriminates identified for each serogroup. This work provides a potential diagnostic platform that can reduce diagnostic limitations for the detection of non-O157 serogroups."]},{"key":"dc:title","label":"Title","values":["MOL-PCR For SNP Based Detection and Characterization of STEC non-O157 Strains"]}]}],"canonical_facts":{"dc:creator":["Mendez, Heather M."],"dc:description.abstract":["Shiga toxin-producing Escherichia coli (STEC) strains are important food-borne pathogens that have been linked to human illness. In the United States, STEC O157:H7 strains have been attributed to the majority of disease and outbreaks; however, the frequency of disease associated with STEC non-O157 strains have also increased and is often under recognized due to diagnostic limitations. In 2012, the Food Safety and Inspection Service (FSIS) implemented mandatory testing for non-O157 STEC serogroups O26, O45, O103, O111, O121, and O145. Single nucleotide polymorphisms (SNPs) have been identified in the O-antigen gene cluster for each of the non-O157 STEC serogroups that can differentiate between a STEC and non-STEC strain. In this study, the multiplex oligonucleotide ligation-PCR (MOL-PCR) detection platform was adapted for the detection and characterization of the above-described non-O157 STEC serogroups. The SNP-based MOL-PCR assay has demonstrated the ability to simultaneously detect the non-O157 serogroups and characterize the strain based on the SNP discriminates identified for each serogroup. This work provides a potential diagnostic platform that can reduce diagnostic limitations for the detection of non-O157 serogroups."],"dc:identifier":["http://hdl.handle.net/1928/31682","https://digitalrepository.unm.edu/bme_etds/2"],"dc:language":["English"],"dc:subject":["Detection of STEC non-O157 Serogroups"],"dc:title":["MOL-PCR For SNP Based Detection and Characterization of STEC non-O157 Strains"],"thesis:degree_discipline":["Biomedical Engineering"],"thesis:degree_level":["Thesis","Masters"],"thesis:degree_name":["Biomedical Engineering"]},"updated_at":"2026-07-24T05:25:53Z"}