{"id":{"repo_id":"unlv","oai_identifier":"oai:oasis.library.unlv.edu:rtds-2593"},"canonical_url":"https://search.dev.ndltd.org/etd/unlv/oai:oasis.library.unlv.edu:rtds-2593","repository":{"repo_id":"unlv","name":"University of Nevada - Las Vegas","base_url":"https://oasis.library.unlv.edu/do/oai/"},"display":{"title":"Cytotoxic effects of electron carriers are inhibited by Hsp27 in breast cancer cell lines","abstract":"Human breast cancer cell lines DC4, DB46, MCF-7, MDA-MD-435 were incubated with cytochrome c at 5 x 10-7M, 5 x 10 -6M and 5 x 10-5M. Human colon cancer cell line, RKO, and normal human lung fibroblast cell line, HFL1, were incubated with cytochrome c at 5.6 x 10-8M, 5.6 x 10-7M and 5.6 x 10-6M. Cytochrome c exposure showed a dose response including cell death in breast cancer cell lines. RKO showed no sensitivity to cytochrome c exposure at any of the concentrations and HFL1 showed sensitivity only at the highest concentration. FAD, NADH, Q10 and ATP were incubated with cells at 1 x 10 -9M, 1 x 10-8M and 1 x 10 -7M. Incubation of FAD with DC4 and DB46 was also done at 5 x 10-7M, 5 x 10-6M and 5 x 10-5M. FAD, ATP and NADH were more effective in cell death induction at much lower concentrations than cytochrome c. Q 10 was least effective in cell death induction.","abstract_html":"Human breast cancer cell lines DC4, DB46, MCF-7, MDA-MD-435 were incubated with cytochrome c at 5 x 10-7M, 5 x 10 -6M and 5 x 10-5M. Human colon cancer cell line, RKO, and normal human lung fibroblast cell line, HFL1, were incubated with cytochrome c at 5.6 x 10-8M, 5.6 x 10-7M and 5.6 x 10-6M. Cytochrome c exposure showed a dose response including cell death in breast cancer cell lines. RKO showed no sensitivity to cytochrome c exposure at any of the concentrations and HFL1 showed sensitivity only at the highest concentration. FAD, NADH, Q10 and ATP were incubated with cells at 1 x 10 -9M, 1 x 10-8M and 1 x 10 -7M. Incubation of FAD with DC4 and DB46 was also done at 5 x 10-7M, 5 x 10-6M and 5 x 10-5M. FAD, ATP and NADH were more effective in cell death induction at much lower concentrations than cytochrome c. Q 10 was least effective in cell death induction.","abstract_has_math":false,"creators":["Giraud, Yvonne Carina"],"institution":"University of Nevada, Las Vegas","degree_name":"Master of Science (MS)","degree_level":"Thesis","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":["Stephen W. Carper"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2003,"date_issued":"2003-01-01T08:00:00Z","date_published":"2003-01-01T08:00:00Z","updated_at":"2026-07-24T05:25:40Z","subjects":[],"languages":["English"],"rights":["IN COPYRIGHT. For more information about this rights statement, please visit http://rightsstatements.org/vocab/InC/1.0/"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://oasis.library.unlv.edu/rtds/1594"],"render_values":[{"text":"https://oasis.library.unlv.edu/rtds/1594","href":"https://oasis.library.unlv.edu/rtds/1594","code":true}]}]},"links":{"outbound_url":"https://doi.org/10.25669/nepm-dtf0","outbound_label":"DOI","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Stephen W. 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For more information about this rights statement, please visit http://rightsstatements.org/vocab/InC/1.0/"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["10.25669/nepm-dtf0","https://oasis.library.unlv.edu/rtds/1594","https://oasis.library.unlv.edu/context/rtds/article/2593/viewcontent/uc.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Human breast cancer cell lines DC4, DB46, MCF-7, MDA-MD-435 were incubated with cytochrome c at 5 x 10-7M, 5 x 10 -6M and 5 x 10-5M. Human colon cancer cell line, RKO, and normal human lung fibroblast cell line, HFL1, were incubated with cytochrome c at 5.6 x 10-8M, 5.6 x 10-7M and 5.6 x 10-6M. Cytochrome c exposure showed a dose response including cell death in breast cancer cell lines. RKO showed no sensitivity to cytochrome c exposure at any of the concentrations and HFL1 showed sensitivity only at the highest concentration. FAD, NADH, Q10 and ATP were incubated with cells at 1 x 10 -9M, 1 x 10-8M and 1 x 10 -7M. Incubation of FAD with DC4 and DB46 was also done at 5 x 10-7M, 5 x 10-6M and 5 x 10-5M. FAD, ATP and NADH were more effective in cell death induction at much lower concentrations than cytochrome c. Q 10 was least effective in cell death induction."]},{"key":"dc:format","label":"Dc Format","values":["pdf"]},{"key":"dc:title","label":"Title","values":["Cytotoxic effects of electron carriers are inhibited by Hsp27 in breast cancer cell lines"]}]}],"canonical_facts":{"dc:contributor":["Stephen W. Carper"],"dc:creator":["Giraud, Yvonne Carina"],"dc:description.abstract":["Human breast cancer cell lines DC4, DB46, MCF-7, MDA-MD-435 were incubated with cytochrome c at 5 x 10-7M, 5 x 10 -6M and 5 x 10-5M. Human colon cancer cell line, RKO, and normal human lung fibroblast cell line, HFL1, were incubated with cytochrome c at 5.6 x 10-8M, 5.6 x 10-7M and 5.6 x 10-6M. Cytochrome c exposure showed a dose response including cell death in breast cancer cell lines. RKO showed no sensitivity to cytochrome c exposure at any of the concentrations and HFL1 showed sensitivity only at the highest concentration. FAD, NADH, Q10 and ATP were incubated with cells at 1 x 10 -9M, 1 x 10-8M and 1 x 10 -7M. Incubation of FAD with DC4 and DB46 was also done at 5 x 10-7M, 5 x 10-6M and 5 x 10-5M. FAD, ATP and NADH were more effective in cell death induction at much lower concentrations than cytochrome c. Q 10 was least effective in cell death induction."],"dc:format":["pdf"],"dc:identifier":["10.25669/nepm-dtf0","https://oasis.library.unlv.edu/rtds/1594","https://oasis.library.unlv.edu/context/rtds/article/2593/viewcontent/uc.pdf"],"dc:language":["English"],"dc:publisher":["University of Nevada, Las Vegas"],"dc:rights":["IN COPYRIGHT. For more information about this rights statement, please visit http://rightsstatements.org/vocab/InC/1.0/"],"dc:title":["Cytotoxic effects of electron carriers are inhibited by Hsp27 in breast cancer cell lines"],"dc:type":["Text"],"thesis:degree_discipline":["Chemistry"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["Master of Science (MS)"]},"updated_at":"2026-07-24T05:25:40Z"}