{"id":{"repo_id":"unlv","oai_identifier":"oai:oasis.library.unlv.edu:rtds-2303"},"canonical_url":"https://search.dev.ndltd.org/etd/unlv/oai:oasis.library.unlv.edu:rtds-2303","repository":{"repo_id":"unlv","name":"University of Nevada - Las Vegas","base_url":"https://oasis.library.unlv.edu/do/oai/"},"display":{"title":"Characterization of perillyl alcohol as a cell migration-inhibiting compound","abstract":"Inhibiting cancer cell migration could greatly improve the prognosis of cancer patients. Prior to commercialization of the Plopper laboratory designed a 96-well plate, we optimized our in vitro migration and cell viability assays to meet the standards set by the National Cancer Institute for use in a large-scale drug screen for anti-migration compounds. Using these assays, we measured the cytotoxicity and migration-inhibiting effect of dimethyl sulfuoxide, a drug solvent, in three cancer and one non-cancer cell lines. Next, we investigated plant-derived compounds called monoterpenes, known chemopreventive/chemotherapeutic agents, for non-cytotoxic, migration-inhibiting properties in MCF-10A normal and MDA-MB 435 cancerous breast cells. Perillyl alcohol at 0.5 mM inhibited normal breast cell migration while it failed to affect cancer cell migration. Finally, we examined the loss of prenylation of RhoA, a small GTPase protein involved in the formation of focal adhesions and stress fibers, and corresponding morphological changes following exposure to perillyl alcohol.","abstract_html":"Inhibiting cancer cell migration could greatly improve the prognosis of cancer patients. Prior to commercialization of the Plopper laboratory designed a 96-well plate, we optimized our in vitro migration and cell viability assays to meet the standards set by the National Cancer Institute for use in a large-scale drug screen for anti-migration compounds. Using these assays, we measured the cytotoxicity and migration-inhibiting effect of dimethyl sulfuoxide, a drug solvent, in three cancer and one non-cancer cell lines. Next, we investigated plant-derived compounds called monoterpenes, known chemopreventive/chemotherapeutic agents, for non-cytotoxic, migration-inhibiting properties in MCF-10A normal and MDA-MB 435 cancerous breast cells. Perillyl alcohol at 0.5 mM inhibited normal breast cell migration while it failed to affect cancer cell migration. Finally, we examined the loss of prenylation of RhoA, a small GTPase protein involved in the formation of focal adhesions and stress fibers, and corresponding morphological changes following exposure to perillyl alcohol.","abstract_has_math":false,"creators":["Wagner, Johanna Elizabeth"],"institution":"University of Nevada, Las Vegas","degree_name":"Master of Science (MS)","degree_level":"Thesis","degree_discipline":"Biological Science","degree_department":null,"school":null,"contributors":["George Plopper"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2001,"date_issued":"2001-01-01T08:00:00Z","date_published":"2001-01-01T08:00:00Z","updated_at":"2026-07-24T05:25:26Z","subjects":[],"languages":["English"],"rights":["IN COPYRIGHT. For more information about this rights statement, please visit http://rightsstatements.org/vocab/InC/1.0/"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://oasis.library.unlv.edu/rtds/1304"],"render_values":[{"text":"https://oasis.library.unlv.edu/rtds/1304","href":"https://oasis.library.unlv.edu/rtds/1304","code":true}]}]},"links":{"outbound_url":"https://doi.org/10.25669/6gbj-lg4j","outbound_label":"DOI","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["George Plopper"]},{"key":"dc:creator","label":"Author","values":["Wagner, Johanna Elizabeth"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:publisher","label":"Institution","values":["University of Nevada, Las Vegas"]},{"key":"dc:type","label":"Dc Type","values":["Text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biological Science"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science (MS)"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["English"]},{"key":"dc:rights","label":"Dc Rights","values":["IN COPYRIGHT. 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Using these assays, we measured the cytotoxicity and migration-inhibiting effect of dimethyl sulfuoxide, a drug solvent, in three cancer and one non-cancer cell lines. Next, we investigated plant-derived compounds called monoterpenes, known chemopreventive/chemotherapeutic agents, for non-cytotoxic, migration-inhibiting properties in MCF-10A normal and MDA-MB 435 cancerous breast cells. Perillyl alcohol at 0.5 mM inhibited normal breast cell migration while it failed to affect cancer cell migration. Finally, we examined the loss of prenylation of RhoA, a small GTPase protein involved in the formation of focal adhesions and stress fibers, and corresponding morphological changes following exposure to perillyl alcohol."]},{"key":"dc:format","label":"Dc Format","values":["pdf"]},{"key":"dc:title","label":"Title","values":["Characterization of perillyl alcohol as a cell migration-inhibiting compound"]}]}],"canonical_facts":{"dc:contributor":["George Plopper"],"dc:creator":["Wagner, Johanna Elizabeth"],"dc:description.abstract":["Inhibiting cancer cell migration could greatly improve the prognosis of cancer patients. Prior to commercialization of the Plopper laboratory designed a 96-well plate, we optimized our in vitro migration and cell viability assays to meet the standards set by the National Cancer Institute for use in a large-scale drug screen for anti-migration compounds. Using these assays, we measured the cytotoxicity and migration-inhibiting effect of dimethyl sulfuoxide, a drug solvent, in three cancer and one non-cancer cell lines. Next, we investigated plant-derived compounds called monoterpenes, known chemopreventive/chemotherapeutic agents, for non-cytotoxic, migration-inhibiting properties in MCF-10A normal and MDA-MB 435 cancerous breast cells. Perillyl alcohol at 0.5 mM inhibited normal breast cell migration while it failed to affect cancer cell migration. Finally, we examined the loss of prenylation of RhoA, a small GTPase protein involved in the formation of focal adhesions and stress fibers, and corresponding morphological changes following exposure to perillyl alcohol."],"dc:format":["pdf"],"dc:identifier":["10.25669/6gbj-lg4j","https://oasis.library.unlv.edu/rtds/1304","https://oasis.library.unlv.edu/context/rtds/article/2303/viewcontent/uc.pdf"],"dc:language":["English"],"dc:publisher":["University of Nevada, Las Vegas"],"dc:rights":["IN COPYRIGHT. For more information about this rights statement, please visit http://rightsstatements.org/vocab/InC/1.0/"],"dc:title":["Characterization of perillyl alcohol as a cell migration-inhibiting compound"],"dc:type":["Text"],"thesis:degree_discipline":["Biological Science"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["Master of Science (MS)"]},"updated_at":"2026-07-24T05:25:26Z"}