Detection of glial interleukin-6 release: Development of a novel interleukin-6 Elisa

The development of an interleukin-6 (IL-6) enzyme-linked immunosorbent (ELISA) assay resulted in a detection range of 1 ng/m1 to 10 ng/mL IL-6. The limit of detection (LOD) for IL-6 was 0.25 ng/mL. Troubleshooting techniques were attempted including matrix effects, non-specific binding, and coating buffer pH differences. In comparison to the ELISA, the LOD for IL-6 using the 7TD1 bioassay was 2--5 pg/mL. Therefore the 7TD1 bioassay was implemented for the experiments described in this study. IL-6 is a cytokine known to stimulate B-cell differentiation and to activate T-cells. IL-6 is elevated in inflammatory and neurodegenerative diseases. Interleukin-1beta (IL-1beta) stimulates IL-6 release from glial cells. Catecholamines and IL-1beta synergistically release IL-6 from glial cells. In the rat C6 glioma cell line, the role of indoleamines was examined alone and in combination with IL-1beta resulting in no synergistic effects on IL-6 release. Isoproterenol synergistically acted with IL-1beta on stimulating IL-6 release. IL-1 receptor antagonist decreased the IL-1beta stimulation of IL-6. These results demonstrate that the catecholamines and IL-1beta play a role in the regulation of IL-6 expression.

Name thesis:degree_name

Master of Science (MS)

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Chemistry

Grantor dc:publisher

University of Nevada, Las Vegas

Year

1999

Author dc:creator

• Bowman, Kay-Lynn

Contributors dc:contributor

• Bryan L. Spangelo

Statement dc:rights

• IN COPYRIGHT. For more information about this rights statement, please visit http://rightsstatements.org/vocab/InC/1.0/

Language dc:language

English

Identifier

https://oasis.library.unlv.edu/rtds/1070

OAI identifier oai:identifier

oai:oasis.library.unlv.edu:rtds-2069