The influence of blood flow on angiogenesis in the 3-day chick embryo

I hypothesized that experimental elimination of blood flow via ligation of the conotruncus arteriosus would cause a cessation of blood vessel development and even vessel regression. India ink, injected to highlight the vasculature, had no effect on blood flow. Embryos were divided into ligated, sham, and control groups. After ligature placement and 6 hr. reincubation, KCl, added topically induced cardiac arrest. The diameters of the anterior cardinal vein, anterior vitelline vein, vitelline artery, and vitelline vein were not statistically different from sham and control values. Eye diameter increased equally in the presence and absence of blood flow. These results demonstrate that blood flow had no influence on vessel growth in diameter during a 6 hr. period. Blood flow did appear to offer a stabilizing influence against vessel degradation. A critical window of 6 hr. may exist during which the absence of blood flow has no detrimental effects on embryonic vasculature.

Name thesis:degree_name

Master of Science (MS)

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Biological Science

Grantor dc:publisher

University of Nevada, Las Vegas

Year

1998

Author dc:creator

• Clarke, Brett Mark

Contributors dc:contributor

• Carl L. Reiber

Statement dc:rights

• IN COPYRIGHT. For more information about this rights statement, please visit http://rightsstatements.org/vocab/InC/1.0/

Language dc:language

English

Identifier

https://oasis.library.unlv.edu/rtds/955

OAI identifier oai:identifier

oai:oasis.library.unlv.edu:rtds-1954