A new approach to analysis with chemiluminescent detection is proposed and demonstrated. Two Phase Chemiluminescence utilizes an immobilized reagent bulk phase and a sample phase which contains the analyte. The analyte is permitted to diffuse into the reagent phase under controlled conditions. A chemiluminescent reaction occurs in the reagent phase, which is quantitated and related to the analyte concentration in the sample phase. A general theory is presented to describe the relationship between analyte concentration and chemiluminescent intensity.
The reaction between luminol and hydrogen peroxide was used in a probe configuration. The reaction was catalyzed by peroxidase immobilized on one end of the fiber optic. A non-linear response for hydrogen peroxide was found at a pH of 9 and 10('-3) M luminol concentration. The detection limit for peroxidase was found to be 10('-6) M.
The reaction between adenosine triphosphate (ATP) and firefly luciferine was monitored in a flow cell configuration. The firefly luciferase was immobilized in the presence of luciferin. A detection limit for ATP under nonoptimized conditions was estimated at 2x10('-8) M ATP. With the current design, a calibration curve for ATP concentration could not be constructed due to the decay of the signal intensity with exposure of the reagent phase to ATP.
The chemiluminescence of a peramino ethylene; 1,1',3,3', tetraethyl (DELTA)('2,2')bi(imidazolidine) (EIA); was used to develop an oxygen sensor. A 10% EIA solution in the hexane was immobilized behind a Teflon membrane. Oxygen diffusing through the membrane reacted with the EIA producing a signal proportional to the oxygen partial pressure in both the gaseous and aqueous phases. The detection limit for oxygen in the gas phase was estimated to be about 1 ppm(V/V).
","abstract_html":"<p>A new approach to analysis with chemiluminescent detection is proposed and demonstrated. Two Phase Chemiluminescence utilizes an immobilized reagent bulk phase and a sample phase which contains the analyte. The analyte is permitted to diffuse into the reagent phase under controlled conditions. A chemiluminescent reaction occurs in the reagent phase, which is quantitated and related to the analyte concentration in the sample phase. A general theory is presented to describe the relationship between analyte concentration and chemiluminescent intensity.</p><p>The reaction between luminol and hydrogen peroxide was used in a probe configuration. The reaction was catalyzed by peroxidase immobilized on one end of the fiber optic. A non-linear response for hydrogen peroxide was found at a pH of 9 and 10('-3) M luminol concentration. The detection limit for peroxidase was found to be 10('-6) M.</p><p>The reaction between adenosine triphosphate (ATP) and firefly luciferine was monitored in a flow cell configuration. The firefly luciferase was immobilized in the presence of luciferin. A detection limit for ATP under nonoptimized conditions was estimated at 2x10('-8) M ATP. With the current design, a calibration curve for ATP concentration could not be constructed due to the decay of the signal intensity with exposure of the reagent phase to ATP.</p><p>The chemiluminescence of a peramino ethylene; 1,1',3,3', tetraethyl (DELTA)('2,2')bi(imidazolidine) (EIA); was used to develop an oxygen sensor. A 10% EIA solution in the hexane was immobilized behind a Teflon membrane. Oxygen diffusing through the membrane reacted with the EIA producing a signal proportional to the oxygen partial pressure in both the gaseous and aqueous phases. The detection limit for oxygen in the gas phase was estimated to be about 1 ppm(V/V).</p>","abstract_has_math":false,"creators":["FREEMAN, THOMPSON MARSH"],"institution":null,"degree_name":"Doctor of Philosophy","degree_level":"Dissertation","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1981,"date_issued":"1981-01-01T08:00:00Z","date_published":"1981-01-01T08:00:00Z","updated_at":"2026-07-24T05:23:09Z","subjects":["Chemistry","Analytical"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://scholars.unh.edu/dissertation/1290","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["FREEMAN, THOMPSON MARSH"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Doctor of Philosophy"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Chemistry","Analytical"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://scholars.unh.edu/dissertation/1290"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["A new approach to analysis with chemiluminescent detection is proposed and demonstrated. Two Phase Chemiluminescence utilizes an immobilized reagent bulk phase and a sample phase which contains the analyte. The analyte is permitted to diffuse into the reagent phase under controlled conditions. A chemiluminescent reaction occurs in the reagent phase, which is quantitated and related to the analyte concentration in the sample phase. A general theory is presented to describe the relationship between analyte concentration and chemiluminescent intensity.
The reaction between luminol and hydrogen peroxide was used in a probe configuration. The reaction was catalyzed by peroxidase immobilized on one end of the fiber optic. A non-linear response for hydrogen peroxide was found at a pH of 9 and 10('-3) M luminol concentration. The detection limit for peroxidase was found to be 10('-6) M.
The reaction between adenosine triphosphate (ATP) and firefly luciferine was monitored in a flow cell configuration. The firefly luciferase was immobilized in the presence of luciferin. A detection limit for ATP under nonoptimized conditions was estimated at 2x10('-8) M ATP. With the current design, a calibration curve for ATP concentration could not be constructed due to the decay of the signal intensity with exposure of the reagent phase to ATP.
The chemiluminescence of a peramino ethylene; 1,1',3,3', tetraethyl (DELTA)('2,2')bi(imidazolidine) (EIA); was used to develop an oxygen sensor. A 10% EIA solution in the hexane was immobilized behind a Teflon membrane. Oxygen diffusing through the membrane reacted with the EIA producing a signal proportional to the oxygen partial pressure in both the gaseous and aqueous phases. The detection limit for oxygen in the gas phase was estimated to be about 1 ppm(V/V).
"]},{"key":"dc:title","label":"Title","values":["CHEMILUMINESCENCE ANALYSIS BASED ON TWO PHASE REACTANT SYSTEMS"]}]}],"canonical_facts":{"dc:creator":["FREEMAN, THOMPSON MARSH"],"dc:description.abstract":["A new approach to analysis with chemiluminescent detection is proposed and demonstrated. Two Phase Chemiluminescence utilizes an immobilized reagent bulk phase and a sample phase which contains the analyte. The analyte is permitted to diffuse into the reagent phase under controlled conditions. A chemiluminescent reaction occurs in the reagent phase, which is quantitated and related to the analyte concentration in the sample phase. A general theory is presented to describe the relationship between analyte concentration and chemiluminescent intensity.
The reaction between luminol and hydrogen peroxide was used in a probe configuration. The reaction was catalyzed by peroxidase immobilized on one end of the fiber optic. A non-linear response for hydrogen peroxide was found at a pH of 9 and 10('-3) M luminol concentration. The detection limit for peroxidase was found to be 10('-6) M.
The reaction between adenosine triphosphate (ATP) and firefly luciferine was monitored in a flow cell configuration. The firefly luciferase was immobilized in the presence of luciferin. A detection limit for ATP under nonoptimized conditions was estimated at 2x10('-8) M ATP. With the current design, a calibration curve for ATP concentration could not be constructed due to the decay of the signal intensity with exposure of the reagent phase to ATP.
The chemiluminescence of a peramino ethylene; 1,1',3,3', tetraethyl (DELTA)('2,2')bi(imidazolidine) (EIA); was used to develop an oxygen sensor. A 10% EIA solution in the hexane was immobilized behind a Teflon membrane. Oxygen diffusing through the membrane reacted with the EIA producing a signal proportional to the oxygen partial pressure in both the gaseous and aqueous phases. The detection limit for oxygen in the gas phase was estimated to be about 1 ppm(V/V).
"],"dc:identifier":["https://scholars.unh.edu/dissertation/1290"],"dc:subject":["Chemistry","Analytical"],"dc:title":["CHEMILUMINESCENCE ANALYSIS BASED ON TWO PHASE REACTANT SYSTEMS"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Doctor of Philosophy"]},"updated_at":"2026-07-24T05:23:09Z"}