{"id":{"repo_id":"umn","oai_identifier":"oai:conservancy.umn.edu:11299/278804"},"canonical_url":"https://search.dev.ndltd.org/etd/umn/oai:conservancy.umn.edu:11299/278804","repository":{"repo_id":"umn","name":"University of Minnesota","base_url":"https://conservancy.umn.edu/server/oai/request"},"display":{"title":"Regulation mechanisms in molecular motors: engineering bioinspired transportation at micron-scale","abstract":"Intracellular cargo transport, orchestrated by teams of molecular motor proteins on cytoskeletal tracks, is a fundamental process within eukaryotic cells. This thesis presents a comprehensive investigation into multi-motor cargo transport, employing an array of cutting-edge tools, including instrumentational, computational, and DNA-based single-molecule methods. These diverse approaches converge to unveil unprecedented insights into the coordination mechanisms of molecular motors and the regulation of intracellular cargo transport. The research explores the impact of various mechanical and biochemical factors, including cargo-motor linkage stiffness, the influence of adapter proteins like GIPC, and cargo-motor interaction kinetics, on multi-motor cargo dynamics. Through detailed analyses, the interplay of these factors is deciphered, shedding light on the intricate regulation of cargo transport, including pausing phenomena and forces acting on the actin network. Notably, this work extends its focus beyond fundamental biology to propose novel avenues for engineering bioinspired transportation systems at the micron-scale and beyond. The insights gained from this research not only contribute to our understanding of cellular complexities but also highlight promising solutions to micro-scale transport challenges. Moreover, this thesis provides a glimpse into emerging tools and methodologies that hold the potential to revolutionize the field, opening up new horizons for realizing efficient and precise transportation at micron and sub-micron scales. As we continue to unlock the mysteries of intracellular cargo transport, the integration of these multidisciplinary approaches paves the way for groundbreaking innovations in diverse domains, from drug delivery to nanotechnology, and beyond.","abstract_html":"Intracellular cargo transport, orchestrated by teams of molecular motor proteins on cytoskeletal tracks, is a fundamental process within eukaryotic cells. This thesis presents a comprehensive investigation into multi-motor cargo transport, employing an array of cutting-edge tools, including instrumentational, computational, and DNA-based single-molecule methods. These diverse approaches converge to unveil unprecedented insights into the coordination mechanisms of molecular motors and the regulation of intracellular cargo transport. The research explores the impact of various mechanical and biochemical factors, including cargo-motor linkage stiffness, the influence of adapter proteins like GIPC, and cargo-motor interaction kinetics, on multi-motor cargo dynamics. Through detailed analyses, the interplay of these factors is deciphered, shedding light on the intricate regulation of cargo transport, including pausing phenomena and forces acting on the actin network. Notably, this work extends its focus beyond fundamental biology to propose novel avenues for engineering bioinspired transportation systems at the micron-scale and beyond. The insights gained from this research not only contribute to our understanding of cellular complexities but also highlight promising solutions to micro-scale transport challenges. Moreover, this thesis provides a glimpse into emerging tools and methodologies that hold the potential to revolutionize the field, opening up new horizons for realizing efficient and precise transportation at micron and sub-micron scales. As we continue to unlock the mysteries of intracellular cargo transport, the integration of these multidisciplinary approaches paves the way for groundbreaking innovations in diverse domains, from drug delivery to nanotechnology, and beyond.","abstract_has_math":false,"creators":["Shrivastava, Rachit"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2023,"date_issued":"2023-09","date_published":"2023-09","updated_at":"2026-07-24T05:19:44Z","subjects":["Bioinspired Engineering","Computational Biology","Intracellular Transportation","Molecular Motors","Myosin","Optical Tweezers"],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/11299/278804","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Shrivastava, Rachit"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2026-02-12T17:45:46Z"]},{"key":"dc:date.issued","label":"Date","values":["2023-09"]},{"key":"dc:type","label":"Dc Type","values":["Thesis or Dissertation"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Bioinspired Engineering","Computational Biology","Intracellular Transportation","Molecular Motors","Myosin","Optical Tweezers"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/11299/278804"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["University of Minnesota Ph.D. dissertation. September 2023. Major: Electrical Engineering. Advisor: Murti Salapaka. 1 computer file (PDF); xv, 88 pages."]},{"key":"dc:description.abstract","label":"Abstract","values":["Intracellular cargo transport, orchestrated by teams of molecular motor proteins on cytoskeletal tracks, is a fundamental process within eukaryotic cells. This thesis presents a comprehensive investigation into multi-motor cargo transport, employing an array of cutting-edge tools, including instrumentational, computational, and DNA-based single-molecule methods. These diverse approaches converge to unveil unprecedented insights into the coordination mechanisms of molecular motors and the regulation of intracellular cargo transport. The research explores the impact of various mechanical and biochemical factors, including cargo-motor linkage stiffness, the influence of adapter proteins like GIPC, and cargo-motor interaction kinetics, on multi-motor cargo dynamics. Through detailed analyses, the interplay of these factors is deciphered, shedding light on the intricate regulation of cargo transport, including pausing phenomena and forces acting on the actin network. Notably, this work extends its focus beyond fundamental biology to propose novel avenues for engineering bioinspired transportation systems at the micron-scale and beyond. The insights gained from this research not only contribute to our understanding of cellular complexities but also highlight promising solutions to micro-scale transport challenges. Moreover, this thesis provides a glimpse into emerging tools and methodologies that hold the potential to revolutionize the field, opening up new horizons for realizing efficient and precise transportation at micron and sub-micron scales. As we continue to unlock the mysteries of intracellular cargo transport, the integration of these multidisciplinary approaches paves the way for groundbreaking innovations in diverse domains, from drug delivery to nanotechnology, and beyond."]},{"key":"dc:title","label":"Title","values":["Regulation mechanisms in molecular motors: engineering bioinspired transportation at micron-scale"]}]}],"canonical_facts":{"dc:creator":["Shrivastava, Rachit"],"dc:date.accessioned":["2026-02-12T17:45:46Z"],"dc:date.issued":["2023-09"],"dc:description":["University of Minnesota Ph.D. dissertation. September 2023. Major: Electrical Engineering. Advisor: Murti Salapaka. 1 computer file (PDF); xv, 88 pages."],"dc:description.abstract":["Intracellular cargo transport, orchestrated by teams of molecular motor proteins on cytoskeletal tracks, is a fundamental process within eukaryotic cells. This thesis presents a comprehensive investigation into multi-motor cargo transport, employing an array of cutting-edge tools, including instrumentational, computational, and DNA-based single-molecule methods. These diverse approaches converge to unveil unprecedented insights into the coordination mechanisms of molecular motors and the regulation of intracellular cargo transport. The research explores the impact of various mechanical and biochemical factors, including cargo-motor linkage stiffness, the influence of adapter proteins like GIPC, and cargo-motor interaction kinetics, on multi-motor cargo dynamics. Through detailed analyses, the interplay of these factors is deciphered, shedding light on the intricate regulation of cargo transport, including pausing phenomena and forces acting on the actin network. Notably, this work extends its focus beyond fundamental biology to propose novel avenues for engineering bioinspired transportation systems at the micron-scale and beyond. The insights gained from this research not only contribute to our understanding of cellular complexities but also highlight promising solutions to micro-scale transport challenges. Moreover, this thesis provides a glimpse into emerging tools and methodologies that hold the potential to revolutionize the field, opening up new horizons for realizing efficient and precise transportation at micron and sub-micron scales. As we continue to unlock the mysteries of intracellular cargo transport, the integration of these multidisciplinary approaches paves the way for groundbreaking innovations in diverse domains, from drug delivery to nanotechnology, and beyond."],"dc:identifier.uri":["https://hdl.handle.net/11299/278804"],"dc:language.iso":["en"],"dc:subject":["Bioinspired Engineering","Computational Biology","Intracellular Transportation","Molecular Motors","Myosin","Optical Tweezers"],"dc:title":["Regulation mechanisms in molecular motors: engineering bioinspired transportation at micron-scale"],"dc:type":["Thesis or Dissertation"]},"updated_at":"2026-07-24T05:19:44Z"}